Thermoactinomyces khenchelensis sp. nov., a filamentous bacterium isolated from soil sediment of a terrestrial hot spring

Abstract

A novel thermophilic filamentous bacterium, designated strain T36(T), was isolated from soil sediment sample from a hot spring source collected in Khenchela province, Algeria. Strain T36(T) was identified as a member of the genus Thermoactinomyces by a polyphasic approach. Strain T36(T) was observed to form white aerial mycelium and non-coloured to pale yellow substrate mycelium, both producing endospores, sessile or borne by short sporophores. The optimum growth temperature and pH were found to be 37-55 °C and 7.0-9.0, respectively and the optimum NaCl concentration for growth was found to be 0-7 % (w/v). The diagnostic diamino acid in the cell wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinone of strain T36(T) was identified as MK-7 (H0). The major fatty acids were found to be iso-C15:0 and iso-C17:0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphoglycolipid. The chemotaxonomic properties of strain T36(T) are consistent with those shared by members of the genus Thermoactinomyces. 16S rRNA gene sequence analysis indicated that the sequence similarities between strain T36(T) and Thermoactinomyces species with validly published names were less than 98 %. Based on the combined genotypic and phenotypic evidence, it is proposed that strain T36(T) should be classified as representative of a novel species, for which the name Thermoactinomyces khenchelensis sp. nov. is proposed. The type strain is T36(T) (=DSM 45951(T) = CECT 8579(T)).

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

“…Mainly represented by Pyrococcus sp. HT3, Caldicoprobacter algeriensis, and Thermoactinomyces khenchelensis, respectively [74][75][76]. The traditional methods, including morphological, physiological, and biochemical characterization, used for a preliminary classification of the isolates are troublesome, uncertain, difficult for interpretation, and may involve substantial subjective judgment [77].…”

Glyphosate has a negligible impact on bacterial diversity and dynamics during composting

“…The high carbon supply provided by the lignocellulosic residues in the studied mixture requires a steady breakdown of structural sugars and lignin to support the microbial metabolism. Correspondingly, most of the abundant identified genera in both the control and the glyphosate treatment (Figure 2) feature members known to have functions in the transformation of lignocellulosic materials such as Thermobifida , Thermobacillus , Microbacterium , Paenibacillus , Bacillus , Cellvibrio , Streptomyces , Saccharomonospora , Devosia , Solibacillus , Ureibacillus , Thermoactinomyces , Thermomonospora , Brevibacillus , Geobacillus , Demequina , Laceyella , Truepera , Thermobispora , Lysinibacillus , Luteimonas and Sphingobacterium which are capable of degrading cellulose, hemicelluloses or both types of structural sugars (Albuquerque et al, 2018; Ali et al, 2019; Han et al, 2018; Huang et al, 2012; Irfan et al, 2018; Liolios et al, 2010; Lykidis et al, 2007; Meng et al, 2009; Mokrane et al, 2016; Morohoshi et al, 2012; Photphisutthiphong & Vatanyoopaisarn, 2019; Rawway et al, 2018; Roberts et al, 1990; Singh et al, 2013; Stutzenberger, 1972; Suarez et al, 2014; Touzel & Prensier, 2015; Wang et al, 2011). Some others, such as Microbacterium , Thermobifida , Brevibacillus , Ureibacillus , Thermomonospora , Pseudomonas , Streptomyces , Sphingobacterium and Pedomicrobium , have the required machinery to degrade lignin (Chen et al, 2015; Hadad et al, 2005; Larsen et al, 1999; Majumdar et al, 2014; Manaia & Moore, 2002; Okuda et al, 2008; Rahmanpour et al, 2016; Stein, 2014; Taylor et al, 2012).…”

Draft Genome Sequence of Thermoactinomyces vulgaris Strain AGRTWHS02, Isolated from Pasture Soil of a Sheep Dairy Farm in New Zealand

“…The type species of Thermoactinomyces , Thermoactinomyces vulgaris DSM 43016, was first isolated from decaying straw and manure ( 3 ). Since then, a number of other species of Thermoactinomyces have been isolated from a wide range of environments ( 4 – 6 ). Due to their ability to grow at high temperatures, Thermoactinomyces species have been a source of thermostable enzymes ( 7 , 8 ).…”