Thermo-sensitive chitosan copolymer-gold hybrid nanoparticles as a nanocarrier for delivery of erlotinib

Fathi, Marziyeh; Zangabad, Parham Sahandi; Barar, Jaleh; Aghanejad, Ayuob; Erfan-Niya, Hamid; Omidi, Yadollah

doi:10.1016/j.ijbiomac.2017.08.020

Cited by 69 publications

(20 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fathi and co-workers recently designed chitosan copolymer-gold hybrid NPs loaded with erlotinib (ETB), which was released from the nanosystem in a thermo-responsive manner thanks to the temperature-responsiveness of chitosan copolymer composed of (poly( N -isopropylacrylamide)- co -oleic acid)-g-chitosan ((PNIPAm- co -OA)- g -CS) that presented an LCST of around 36 °C. The successful cytotoxicity investigation in A549 cells validated their potential as an effective anticancer drug carrier [ 117 ]. Au NPs were combined in another study with the copolymer Poly (NIPAAm- co -AAm) that was used to create a collapsible thermo-sensitive nanoshell, which exposed targeting ligands (integrin β1) upon NIR irradiation, enabling cell binding.…”

Section: Biomedical Applications Of Thermal-nanomaterialsmentioning

confidence: 99%

Thermo-Sensitive Nanomaterials: Recent Advance in Synthesis and Biomedical Applications

et al. 2018

View full text Add to dashboard Cite

Progress in nanotechnology has enabled us to open many new fronts in biomedical research by exploiting the peculiar properties of materials at the nanoscale. The thermal sensitivity of certain materials is a highly valuable property because it can be exploited in many promising applications, such as thermo-sensitive drug or gene delivery systems, thermotherapy, thermal biosensors, imaging, and diagnosis. This review focuses on recent advances in thermo-sensitive nanomaterials of interest in biomedical applications. We provide an overview of the different kinds of thermoresponsive nanomaterials, discussing their potential and the physical mechanisms behind their thermal response. We thoroughly review their applications in biomedicine and finally discuss the current challenges and future perspectives of thermal therapies.

show abstract

Section: Biomedical Applications Of Thermal-nanomaterialsmentioning

confidence: 99%

Thermo-Sensitive Nanomaterials: Recent Advance in Synthesis and Biomedical Applications

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The cultured cells were treated with the fluorophore-labeled non-targeted SPIONs, the Ap-conjugated SPIONs alone, and the DOX-loaded Ap-conjugated SPIONs at 37°C in a CO 2 incubator for 2 hours. 12 , 13 The cells were washed (×3) with PBS, and then, trypsinized, centrifuged at 160 ×g for 5 min. The cells pellet re-suspended in PBS and analyzed by FACS Calibur ® flow cytometer (Becton Dickinson, San Jose, CA, USA) using a minimum number of 1.0×10 4 cells per event.…”

Section: Methodsmentioning

confidence: 99%

Mucin-1 aptamer-armed superparamagnetic iron oxide nanoparticles for targeted delivery of doxorubicin to breast cancer cells

Aghanejad¹,

Babamiri²,

Adibkia³

et al. 2018

Self Cite

View full text Add to dashboard Cite

Introduction: Superparamagnetic iron oxide nanoparticles (SPIONs) can be functionalized with various agents (e.g., targeting and therapeutic agents) and used for targeted imaging/therapy of cancer. In the present study, we engineered doxorubicin (DOX)-conjugated anti-mucin -1 (MUC-1) aptamer (Ap)-armed PEGylated SPIONs for targeted delivery of DOX molecules to the breast cancer MCF-7 cells. Methods: The SPIONs were synthesized using the thermal decomposition method and modified by polyethylene glycol (PEG) to maximize their biocompatibility and minimize any undesired cytotoxicity effects. Subsequently, DOX molecules were loaded onto the SPIONs, which were further armed with amine-modified MUC-1 aptamer by EDC/NHS chemistry. Results: The morphologic and size analyses of nanoparticles (NPs) by transmission electron microscopy (TEM) and dynamic light scattering (DLS) revealed spherical and monodisperse MNPs with a size range of 5-64 nm. The FT-IR spectrophotometry and 1 HNMR analysis confirmed the surface modification of NPs. The cytotoxicity assay of the aptamer-armed MNPs exhibited a higher death rate in the MUC-1 over-expressing MCF-7 cells as compared to the MUC-1 under-expressing MDA-MB-231 cells. The flow cytometry analysis of the engineered Ap-armed SPIONs revealed a higher uptake as compared to the SPIONs alone. Conclusion: Based on our findings, the anti-MUC-1 Ap-armed PEGylated SPIONs loaded with DOX molecules could serve as an effective multifunctional theranostics for simultaneous detection and eradication of MUC-1-positive breast cancer cells.

show abstract

“…Moreover, nanocarriers permit relatively easy decoration with functional (e.g., cell-targeting) moieties, which, in contrast to direct antibody modification, cannot hamper their reactivity. Thermosensitive ( Fathi et al, 2018 ), enzyme-sensitive ( Aluri and Jayakannan, 2017 ), pH-sensitive ( Braunova et al, 2017 ), light-responsive ( Kim H. et al, 2016 ), and combo-stimuli-responsive ( Kashyap et al, 2016 ) nanocarriers can be easily designed to provide a controlled payload release in response to thermal (body, increased tumor temperature, or localized heating), specific enzymatic (e.g., tumor microenvironment or intracellular enzymes) and specific pH microenvironments (e.g., acidic tumor microenvironment or acidifying endocytic vesicles) as well as to external light exposure or any combination of these.…”

Section: Strategies For Intracellular Targeting Of Antibodies Their mentioning

confidence: 99%

Targeted Intracellular Delivery of Antibodies: The State of the Art

et al. 2018

View full text Add to dashboard Cite

A dominant area of antibody research is the extension of the use of this mighty experimental and therapeutic tool for the specific detection of molecules for diagnostics, visualization, and activity blocking. Despite the ability to raise antibodies against different proteins, numerous applications of antibodies in basic research fields, clinical practice, and biotechnology are restricted to permeabilized cells or extracellular antigens, such as membrane or secreted proteins. With the exception of small groups of autoantibodies, natural antibodies to intracellular targets cannot be used within living cells. This excludes the scope of a major class of intracellular targets, including some infamous cancer-associated molecules. Some of these targets are still not druggable via small molecules because of large flat contact areas and the absence of deep hydrophobic pockets in which small molecules can insert and perturb their activity. Thus, the development of technologies for the targeted intracellular delivery of antibodies, their fragments, or antibody-like molecules is extremely important. Various strategies for intracellular targeting of antibodies via protein-transduction domains or their mimics, liposomes, polymer vesicles, and viral envelopes, are reviewed in this article. The pitfalls, challenges, and perspectives of these technologies are discussed.

show abstract

Thermo-sensitive chitosan copolymer-gold hybrid nanoparticles as a nanocarrier for delivery of erlotinib

Cited by 69 publications

References 54 publications

Thermo-Sensitive Nanomaterials: Recent Advance in Synthesis and Biomedical Applications

Thermo-Sensitive Nanomaterials: Recent Advance in Synthesis and Biomedical Applications

Mucin-1 aptamer-armed superparamagnetic iron oxide nanoparticles for targeted delivery of doxorubicin to breast cancer cells

Targeted Intracellular Delivery of Antibodies: The State of the Art

Contact Info

Product

Resources

About