Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection

Abstract

New strategies with high antimicrobial efficacy against multidrug-resistant bacteria are urgently desired. Herein, we describe a smart triple-functional nanostructure, namely TRIDENT (Thermo-Responsive-Inspired Drug-Delivery Nano-Transporter), for reliable bacterial eradication. The robust antibacterial effectiveness is attributed to the integrated fluorescence monitoring and synergistic chemo-photothermal killing. We notice that temperature rises generated by near-infrared irradiation did not only melt the nanotransporter via a phase change mechanism, but also irreversibly damaged bacterial membranes to facilitate imipenem permeation, thus interfering with cell wall biosynthesis and eventually leading to rapid bacterial death. Both in vitro and in vivo evidence demonstrate that even low doses of imipenem-encapsulated TRIDENT could eradicate clinical methicillin-resistant Staphylococcus aureus, whereas imipenem alone had limited effect. Due to rapid recovery of infected sites and good biosafety we envision a universal antimicrobial platform to fight against multidrug-resistant or extremely drug-resistant bacteria.

Synthesis and Antibacterial Properties of Novel ZnMn2O4–Chitosan Nanocomposites

“…The advent of effective and nontoxic antibiotics-undoubtedly one of the supreme achievements in the history of medicine—has brought immense benefits [4]. The ability to cure infections in the preliminary stage without identifying the root cause of the relevant pathogen has preserved countless lives and enabled numerous advances in modern clinical medicine, such as organ transplantation, surgery, chemotherapy, and premature-infant care [5].…”

Broad spectrum antibacterial photodynamic and photothermal therapy achieved with indocyanine green loaded SPIONs under near infrared irradiation

“…PDT and PTT were considered as a promising alternative and complementary therapy for some chemotherapy resistant cancers[40,41]. In recent years, adaptation of PDT and PTT for the treatment of multidrug resistant infections showed quite successful results[42][43][44].In this study, we investigated the antimicrobial and antibiofilm effects of PDT/PTT combination using APTMS@SPION, ICG and ICG-APTMS@SPION nanoparticles and 10 min laser irradiation at 808 nm (output power of 1150 mW). We used three clinically important biofilm producing gram negative bacteria (K.pneumoniae, E.coli, P.aeruginosa) and one gram positive, strong biofilm producing bacteria, S.epidermidis, to assess the influence of surface charge on internalization of nanoparticles as well as the spectrum of PDT/PTT in different bacterial types.…”

Nanomaterials for the Photothermal Killing of Bacteria

“…The antimicrobial activity was observed to only be effective with irradiation using 808-nm light. The PU-Au-PEG nanomaterial used was active under NIR light, biocompatible, and stable, and exhibited an enhanced killing of bacteria [ 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 ].…”