Thermo-Responsive Polymer-siRNA Conjugates Enabling Artificial Control of Gene Silencing around Body Temperature

Honda, Yuto; Onodera, Sayaka; Takemoto, Hiroyuki; Harun, Noor Faizah Che; Nomoto, Takahiro; Matsui, Makoto; Tomoda, Keishiro; Sun, Yudi; Miura, Yutaka; Nishiyama, Nobuhiro

doi:10.1007/s11095-022-03414-8

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…However, the studies required the use of a commercially available Lipofectamine RNAiMAX to aid transfection, suggesting that transfection efficacy of the conjugate needs further optimization. [227] Figure 10. A) Harun et.…”

Section: Grafting Tomentioning

confidence: 99%

Beyond Lipids: Exploring Advances in Polymeric Gene Delivery in the Lipid Nanoparticles Era

Jogdeo,

Siddhanta,

Das

et al. 2024

Advanced Materials

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The recent success of gene therapy during the COVID‐19 pandemic has underscored the importance of effective and safe delivery systems. Complementing lipid‐based delivery systems, polymers present a promising alternative for gene delivery. Significant advances have been made in the recent past, with multiple clinical trials progressing beyond phase I and several companies actively working on polymeric delivery systems which provides assurance that polymeric carriers can soon achieve clinical translation. The massive advantage of structural tunability and vast chemical space of polymers is being actively leveraged to mitigate shortcomings of traditional polycationic polymers and improve the translatability of delivery systems. Tailored polymeric approaches for diverse nucleic acids and for specific subcellular targets are now being designed to improve therapeutic efficacy. This review describes the recent advances in polymer design for improved gene delivery by polyplexes and covalent polymer‐nucleic acid conjugates. The review also offers a brief note on novel computational techniques for improved polymer design. We conclude with an overview of the current state of polymeric gene therapies in the clinic as well as future directions on their translation to the clinic.This article is protected by copyright. All rights reserved

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Section: Grafting Tomentioning

confidence: 99%

Beyond Lipids: Exploring Advances in Polymeric Gene Delivery in the Lipid Nanoparticles Era

Jogdeo,

Siddhanta,

Das

et al. 2024

Advanced Materials

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“…This copolymer undergoes a coil-to-globule transition, allowing for temperature-controlled interaction between siRNA and intracellular proteins involved in gene silencing. 262 International Journal of Nanomedicine 2023:18…”

Section: Thermo-responsive Biomaterialsmentioning

confidence: 99%

A Comprehensive Review of Small Interfering RNAs (siRNAs): Mechanism, Therapeutic Targets, and Delivery Strategies for Cancer Therapy

Zhang,

Chen,

Gan

et al. 2023

IJN

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Small interfering RNA (siRNA) delivery by nanocarriers has been identified as a promising strategy in the study and treatment of cancer. Short nucleotide sequences are synthesized exogenously to create siRNA, which triggers RNA interference (RNAi) in cells and silences target gene expression in a sequence-specific way. As a nucleic acid-based medicine that has gained popularity recently, siRNA exhibits novel potential for the treatment of cancer. However, there are still many obstacles to overcome before clinical siRNA delivery devices can be developed. In this review, we discuss prospective targets for siRNA drug design, explain siRNA drug properties and benefits, and give an overview of the current clinical siRNA therapeutics for the treatment of cancer. Additionally, we introduce the siRNA chemical modifications and delivery systems that are clinically sophisticated and classify bioresponsive materials for siRNA release in a methodical manner. This review will serve as a reference for researchers in developing more precise and efficient targeted delivery systems, promoting ongoing advances in clinical applications.

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“…Honda, Nishiyama, and et al . [ 8 ] report a temperature-responsive polymer-siRNA conjugate for controllable gene silencing at the body temperature. N-Isopropylacrylamide (NIPAAm)-N,N-dimethylacrylamide (DMAA) is used as a carrier to prepare a thermo-responsive polymer-siRNA conjugate, which possesses the property of coil-to-globule phase transition to control the interaction between siRNA and an intracellular gene silencing-related protein.…”

mentioning

confidence: 99%

Nucleic Acid Delivery

Zhang

Shi

2023

Pharm Res

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Thermo-Responsive Polymer-siRNA Conjugates Enabling Artificial Control of Gene Silencing around Body Temperature

Cited by 5 publications

References 22 publications

Beyond Lipids: Exploring Advances in Polymeric Gene Delivery in the Lipid Nanoparticles Era

Beyond Lipids: Exploring Advances in Polymeric Gene Delivery in the Lipid Nanoparticles Era

A Comprehensive Review of Small Interfering RNAs (siRNAs): Mechanism, Therapeutic Targets, and Delivery Strategies for Cancer Therapy

Nucleic Acid Delivery

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