Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography

A one-step procedure based on surface-initiated atom transfer radical polymerization (SI-ATRP) to hydrophilize monodisperse poly(chloromethylstyrene-co-divinylbenzene) beads has been presented in this work, using 2-hydroxyl-3-[4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl]propyl 2-methylacrylate (HTMA) as a monomer. The chain length of the grafted poly(HTMA) was controlled via varying the ratio of HTMA to initiator on the surface of the beads. When using the grafted beads as a stationary phase in hydrophilic interaction chromatography (HILIC), good resolution for nucleobases/nucleosides was obtained with acetonitrile aqueous solution as an eluent; while for phenolic acids and glycosides, they could be eluted and separated in the presence of TFA. The retention time of the solutes increased with the amount of the grafted HTMA. The retention mechanisms of solutes were investigated by the effects of mobile phase composition and buffer pH on the retention of solutes. The results illustrated that the retention behaviors of the tested solutes were dominated by hydrogen bonding interaction and electrostatic interaction. From the chemical structure of the ligands, the modified beads could not only be used as a stationary phase in HILIC, but also act as a useful building block to develop new stationary phases for other chromatographic modes such as affinity media.

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“…According to the nitrogen content, the amount of the grafted HTMA on the beads (mmol/m 2 ) was calculated by the following equation [24]:…”

Section: Preparation and Characterization Of Poly(htma)-grafted Statimentioning

confidence: 99%

Preparation of hydrophilic polymer‐grafted polystyrene beads for hydrophilic interaction chromatography via surface‐initiated atom transfer radical polymerization

Dai

Wei

et al. 2011

J of Separation Science

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“…Their excellent performances make their wide application in chromatography and adsorption fields, ion-exchange resins and imprinted polymer [23][24][25][26][27] . As a type of adsorbent for the potential separation of aromatics/aliphatics, compared with ionic liquid and pervaporation membrane, CPS beads have a lower raw material cost and simple operation conditions.…”

Section: Introductionmentioning

confidence: 99%

Crosslinked polystyrene beads modified with sulfonyl groups and their application in aromatic/aliphatic hydrocarbons separation

Cai

Shi

et al. 2014

Chem. Res. Chin. Univ.

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The separation of aromatic hydrocarbons from aromatic/aliphatic mixtures was investigated with crosslinked polystyrene(CPS) beads modified with sulfonyl groups. Three sulfonating agents, i.e. benzenesulfonyl chloride(BsCl), 4-toluene sulfonyl chloride(TsCl) and methanesulfonyl chloride(MsCl) were used to prepare sulfonyl CPS beads by Friedel-Crafts reaction. The CPS beads modified with BsCl exhibited higher sulfonation rate than those modified with MsCl and TsCl and obtained optimum selectivity in the experiments of toluene/n-heptane separation. Further separation tests with various other aromatic/aliphatic mixtures were carried out at an initial aromatic concentration of 13%(mass fraction) with the results showing that the modified beads have preferential selectivity for aromatic hydrocarbons in all aromatic/aliphatic mixtures, and especially a separation factor of 8.21 and a swelling ratio of 30% for toluene/cyclohexane system. The thermal stability and regeneration test indicate that the used polystyrene beads can be recovered through heat-drying and reused effectively.

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“…Furthermore, column separation method in all-aqueous system using PIPAAm grafted surfaces as stationary phases have been developed and became a powerful tool for biomedical separations [14][15][16][17][18][19]. PIPAAm is well-known to exhibit thermo-responsive soluble-insoluble change across its lower critical solution temperature (LCST) at 32 • C in aqueous solution [20].…”

Section: Introductionmentioning

confidence: 99%

Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

Mizutani

Nagase

Kikuchi

et al. 2010

Journal of Chromatography A

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Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography

Cited by 78 publications

References 26 publications

Preparation of hydrophilic polymer‐grafted polystyrene beads for hydrophilic interaction chromatography via surface‐initiated atom transfer radical polymerization

Preparation of hydrophilic polymer‐grafted polystyrene beads for hydrophilic interaction chromatography via surface‐initiated atom transfer radical polymerization

Crosslinked polystyrene beads modified with sulfonyl groups and their application in aromatic/aliphatic hydrocarbons separation

Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

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