Thermo-mechanical properties of the cell surface assessed by atomic force microscopy

Стародубцева, М. Н.; Yegorenkov, N.I.; Nikitina, I. A.

doi:10.1016/j.micron.2012.04.001

Cited by 14 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…malignant (MCF-7) breast cells were found to have an apparent Young's modulus significantly lower (1.4-1.8 times) than that of their non-malignant (MCF-10A) counterparts at physiological temperature (37 • C) [117]. In our work we studied mechanical properties of human embryonic fibroblasts cells and the cells of human lung adenocarcinoma cell line (A549) [118]. It was found that human embryonic fibroblasts cells were found to have an apparent elastic modulus in 1.4 times higher (260.215.84 kPa) than that of A549 cells (184.312.45 kPa) at the significance level p< 0.001.…”

Section: Afm-experimental Results Of the Elastic Properties Estimationmentioning

confidence: 99%

Mechanical-Mathematical Modelling of Biological Tissue Behaviour

Журавков

Drozd

Romanova

et al. 2014

Springer Proceedings in Mathematics &Amp; Statistics

View full text Add to dashboard Cite

In present work mechanics-mathematical models are constructed to define physical and mechanical properties of biological tissue. These models were adapted to the AFM experimental data so to be a theoretical basis for one. Researched theoretical results are compared with experimental data to confirm sufficiently high level adequacy of offered methodology for definition of physical and mechanical properties biotissue.

show abstract

Section: Afm-experimental Results Of the Elastic Properties Estimationmentioning

confidence: 99%

Mechanical-Mathematical Modelling of Biological Tissue Behaviour

Журавков

Drozd

Romanova

et al. 2014

Springer Proceedings in Mathematics &Amp; Statistics

View full text Add to dashboard Cite

show abstract

“…This study may lunch further mechanism understandings and optimizations for prognosis and prevention of any blood-related disorders, particularly on diagnosis and profiling the inflammatory modifications at different degrees level. Beyond imaging, AFM provides a "real time" study of living organisms, emerging multifunctional application tools such as: morphology, molecular forces 19,20 and mechanical properties 32,33 . However, AFM requires the immobilization of the cells on bare surfaces, which may influence the cell morphology 34 .…”

Section: Discussionmentioning

confidence: 99%

“…However, these are expensive and did not pass the experimental stage. In the last 20 years, atomic force microscopy (AFM) has emerged as a powerful technique for biological research giving ultrahigh resolution structural information and allowing measurements of molecular forces at the single molecule level 19,20 . It is well known that AFM becomes more and more popular into cell biology monitoring any modifications at micro-and nano-level in cell biology 21 .…”

Section: Introductionmentioning

confidence: 99%

Ultra-rapid non-invasive clinical nano-diagnostic of inflammatory diseases

Rusu¹,

Dulebo²,

Curaj³

et al. 2014

Discover. Rep.

View full text Add to dashboard Cite

“…AFM has several advantages such as high spatial resolution, and can be operated in aqueous solution that allows live cells be analyzed in their physiological environments. The elasticity (Young’s modulus) of various cells such as endothelial cells, leukocytes and fibroblasts has been characterized using AFM15161718192021. Due to the working principle of probe indentation, most of the Young’s modulus are measured in the direction normal to cell membranes or substrate planes.…”

mentioning

confidence: 99%

Measurement of in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device

Lin

Wang

Chen

et al. 2016

Sci Rep

View full text Add to dashboard Cite

In various physiological activities, cells experience stresses along their in-plane direction when facing substrate deformation. Capability of continuous monitoring elasticity of live cell layers during a period is highly desired to investigate cell property variation during various transformations under normal or disease states. This paper reports time-lapsed measurement of live cell layer in-plane elasticity using a pressure sensor embedded microfluidic device. The sensor converts pressure-induced deformation of a flexible membrane to electrical signals. When cells are cultured on top of the membrane, flexural rigidity of the composite membrane increases and further changes the output electrical signals. In the experiments, human embryonic lung fibroblast (MRC-5) cells are cultured and analyzed to estimate the in-plane elasticity. In addition, the cells are treated with a growth factor to simulate lung fibrosis to study the effects of cell transformation on the elasticity variation. For comparison, elasticity measurement on the cells by atomic force microscopy (AFM) is also performed. The experimental results confirm highly anisotropic configuration and material properties of cells. Furthermore, the in-plane elasticity can be monitored during the cell transformation after the growth factor stimulation. Consequently, the developed microfluidic device provides a powerful tool to study physical properties of cells for fundamental biophysics and biomedical researches.

show abstract

Thermo-mechanical properties of the cell surface assessed by atomic force microscopy

Cited by 14 publications

References 30 publications

Mechanical-Mathematical Modelling of Biological Tissue Behaviour

Mechanical-Mathematical Modelling of Biological Tissue Behaviour

Ultra-rapid non-invasive clinical nano-diagnostic of inflammatory diseases

Measurement of in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device

Contact Info

Product

Resources

About