Thermally resistant nuclease in Serratia marcescens hinders PCR reactions and degrades PCR products
Jinpei Cai,
Yunyi Zhang,
Xueer Jiang
et al.
Abstract:Polymerase chain reaction (PCR) is an important tool for exogenous gene acquisition and recombinants identification. There exist two problems when using Serratia marcescens as a template for PCR amplification: amplified PCR products are rapidly degraded, and the results of PCR amplification are unstable. The aim of the present work was to elucidate the reasons for this. By mixing PCR products amplified from Escherichia coli DH5α with S. marcescens supernatant or pellet, we found that the DNA‐degrading substanc… Show more
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