Thermally Induced Introduction of Trehalose into Primary Rat Hepatocytes

He, Xiaoming; Amin, Arthi; Fowler, Alex; Toner, Mehmet

doi:10.1089/cpt.2006.4.178

Cited by 29 publications

(31 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S3 on the cellular uptake of fCCM. This slight increase is probably due to the increase in solubility of curcumin in aqueous solution at higher temperature and hyperthermia-induced hyperpermeability of the cell plasma membrane [37,60,61]. The latter is also probably responsible for the slower (although not significantly) uptake of nCCM during hyperthermia between 1 and 1.5 h compared to that without hyperthermia (Fig.…”

Section: Resultsmentioning

confidence: 99%

Thermally responsive nanoparticle-encapsulated curcumin and its combination with mild hyperthermia for enhanced cancer cell destruction

et al. 2014

Self Cite

View full text Add to dashboard Cite

In this study, thermally responsive polymeric nanoparticle-encapsulated curcumin (nCCM) was prepared and characterized. The nCCM is ~22 and 300 nm in diameter at 37 and 22 °C, respectively. The smaller size of the nCCM at 37 °C was found to significantly facilitate its uptake in vitro by human prostate adenocarcinoma PC-3 cancer cells. However, the intracellular nCCM decreases rapidly (rather than plateaus) after reaching its peak at ~1.5 h during a 3-day incubation of the PC-3 cells with nCCM. Moreover, a mild hyperthermia (with negligible cytotoxicity alone) at 43 °C applied between 1 and 1.5 h during the 3-day incubation not only increases the peak uptake but also alters intracellular distribution of nCCM (facilitating its delivery into cell nuclei), which helps to retain a significantly much higher level of intracellular curcumin. These effects of mild hyperthermia could be due in part to the thermal responsiveness of the nCCM: they are more positively charged at 43 °C and can be more easily attracted to the negatively charged nuclear membrane to enter nuclei as a result of electrostatic interaction. Ultimately, a combination of the thermally responsive nCCM and mild hyperthermia significantly enhances the anticancer capability of nCCM, resulting in a more than 7-fold decrease in its inhibitory concentration to reduce cell viability to 50% (IC50). Further mechanistic studies suggest injury pathways associated with heat shock proteins 27 and 70 should contribute to the enhanced cancer cell destruction by inducing cell apoptosis and necrosis. Overall, this study demonstrates the potential of combining mild hyperthermia and thermally responsive nanodrugs such as nCCM for augmented cancer therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

Thermally responsive nanoparticle-encapsulated curcumin and its combination with mild hyperthermia for enhanced cancer cell destruction

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bulk thermal insults are capable of perturbing the membrane for delivery of small molecules but detrimental effects on cell function preclude their implementation 60,61 . Thermal disruption may be more feasible if confined to a localized area (Fig.…”

Section: Towards Precision Membrane Disruptionmentioning

confidence: 99%

“…In live cells it has been shown that leakage begins at 42° C while temperatures above 55° C promote rapid exchange of low-molecular-weight (<1 kDa) molecules 60 . Second, at the opposite end of the spectrum (<0 C), formation of ice crystals can trigger mechanical expansion and cracking of the cell membrane, which may be reparable upon thawing 61 . Third, within the physiological range (0°-40° C), passing the membrane rapidly through thermal phase transitions may lead to the generation of holes, especially at phase domain boundaries 60,61 .…”

mentioning

confidence: 99%

“…Second, at the opposite end of the spectrum (<0 C), formation of ice crystals can trigger mechanical expansion and cracking of the cell membrane, which may be reparable upon thawing 61 . Third, within the physiological range (0°-40° C), passing the membrane rapidly through thermal phase transitions may lead to the generation of holes, especially at phase domain boundaries 60,61 . In electroporation, the electric charging of non-conducting lipid bilayers and ever-present thermal fluctuations conspire to create and expand a heterogeneous population of pores 62 .…”

mentioning

confidence: 99%

See 1 more Smart Citation

In vitro and ex vivo strategies for intracellular delivery

Stewart¹,

Sharei²,

Ding³

et al. 2016

Nature

707

742

View full text Add to dashboard Cite

Although carrier-mediated delivery systems offer promise for nucleic acid transfection in vivo 1,2 , membrane-disruption-based modalities are attractive candidates for universal delivery systems in vitro and ex vivo. In this review, we begin with motivations driving next-generation intracellular delivery strategies and suggest relevant requirements for future systems. Next, a broad overview of current delivery concepts covering salient strengths, challenges and opportunities is presented. Following that, our focus shifts to prevalent mechanisms of membrane disruption and recovery in the context of intracellular delivery. Finally,

show abstract

“…This approach requires the constant production of adenoviral vectors that exhibit significant cytotoxicity, particularly at high multiplicities of infection (Gordon et al 2001;Guo et al 2000;Puhlev et al 2001). Trehalose has also been introduced into mammalian cells or their organelles through engineered or native transmembrane pores Chen et al 2001b;Elliott et al 2006;Eroglu et al 2000;Liu et al 2005), electroporation (Reuss et al 2004;Shirakashi et al 2002), fluid-phase endocytosis (He et al 2006a;Oliver 2004;Wolkers et al 2003), and lipid phase transition (Beattie et al 1997;He et al 2006a). …”

Section: Freeze-dryingmentioning

confidence: 99%