Thermal transitions of red blood cell deformability. Correlation with membrane rheological properties

Hanss, M.; Koutsouris, D.

doi:10.1016/0005-2736(84)90331-6

Cited by 22 publications

(7 citation statements)

References 49 publications

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“…[11][12][13][14][15][16][17] However, there are reports suggesting that the elastic properties of the lipid bilayer cell membrane could also be quite important for overall cell stiffness. 6,13,[18][19][20] In particular, membrane elasticity could be significantly affected by intercalation with specific molecules, such as amphiphilic molecules 6 and stress hormones. molecules and increasing intracellular concentration of adenosine triphosphate, decreased potassium ion flow away from the cell, an increase of the protein phosphorylation level, and, as a result, affecting erythrocyte membrane stability.…”

Section: Discussionmentioning

confidence: 99%

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Żuk¹,

Targosz‐Korecka²,

Szymoński³

2011

IJN

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Background The main function of red blood cells is to transport oxygen to all parts of the body with the help of hemoglobin. Other proteins of the cell membrane can attach xenobiotics (eg, drugs) from the blood and transport them throughout the body. Only drugs able to bind to the membrane of the red blood cell can modify its structure and elastic properties. The morphology and local elastic properties of living red blood cells incubated with drug solutions commonly used in the treatment of severe asthma were studied by atomic force microscopy and nanoindentation with an atomic force microscopy tip. Methods The elasticity modules of native red blood cells, as well as those incubated with two types of drugs, ie, aminophylline and methylprednisolone, were determined from experimentally measured nanoindentation curves. Results The elasticity modules of erythrocytes incubated with aminophylline were substantially higher than those obtained for nonincubated native, ie, healthy, red blood cells. Conclusion The increase of the elasticity module obtained for aminophylline can reduce the cell’s ability to bind oxygen and transport it through capillaries.

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Section: Discussionmentioning

confidence: 99%

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Żuk¹,

Targosz‐Korecka²,

Szymoński³

2011

IJN

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“…RBC shape-recovery time constant (tc) for rat control, sham-operated, and sepsis groups; data for sham-operated and sepsis groups were obtained from animals at 6 or 18 h after surgery. Difference from control, *p Ͻ 0.05; difference from shamoperated, † p Ͻ 0.05. that there are qualitative differences in RBC rheologic behavior between 25 and 37Њ C. Studies of RBC transit behavior through 5-m pores indicate a smooth, monotonic relation between pore transit rate and temperature over this range and an approximately 15% faster transit rate at 37 versus 25Њ C (27). Based on this relatively minor effect of temperature on overall cellular mechanical behavior, we assume that conducting measurements at 25Њ C does not vitiate the in vivo relevance of the resulting data; nevertheless, future studies, at both temperatures, of RBC in sepsis seem warranted.…”

Section: Several Reports Have Indicated An Association Between Sepsismentioning

confidence: 92%

Red Blood Cell Deformability in Sepsis

Başkurt

Gelmont

Meiselman

1998

Am J Respir Crit Care Med

195

134

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The microcirculatory disturbances in sepsis have prompted micropore bulk-filtration studies of red blood cell (RBC) mechanical behavior (i.e., deformability). However, these prior reports may not solely reflect RBC behavior because of possible white blood cell (WBC) occlusion of the filter pores. The present study was designed to examine RBC mechanical alterations in human and experimental sepsis using techniques that are not affected by WBC artifacts. RBC were obtained from adult patients with sepsis and from healthy control donors. RBC were also obtained from Swiss-albino rats in which experimental sepsis was induced via cecal ligation-puncture. Red cell mechanical behavior was tested using a computerized micropore filtration system (CTA) and a laser-diffraction shearing device (LORCA); the latter provides the extent of RBC deformation at various stresses and the time constant for RBC shape recovery. Salient findings include: (1) for human RBC, significantly decreased deformability at fluid shear stresses < 5 Pa (LORCA) yet no differences from control with the CTA; (2) for rat RBC in experimental sepsis, significant decreases of deformability and shape-recovery time constant (LORCA) but no differences with the CTA. We conclude that RBC deformability is reduced in sepsis but that micropore bulk-filtration methods may not be appropriate for detecting these changes.

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“…Flow of erythrocytes through 5 ,um diameter Erythrcyte mean cell volume (fl) 92 88 4 Effect ofalteration in osmolality on erythrocyte mean cell volume and mean cell haemoglobin concentration and Hemorheometre index offitration using 3 pum (-) and S pn (0) diameter straight channel polycarbonate membranes. Index offitration at physiological osmolality (290 mmol (mosmol)lkg) has been expressed as 100%.…”

Section: Erythrocyte Filtrationmentioning

confidence: 99%

“…92 Others have found that the filtration of erythrocytes through 5 ,um pores depends on temperature within the range 32-42°C. 93 Dependency of temperature is, however, affected by the size of pore, the reduction in the filterability of erythrocytes at 20°C compared with 25°C being greater for 3 ,um than for 5 ,um diameter pores.…”

Section: Stuart Other Technical Variables In Fitration Studiesmentioning

confidence: 99%

Erythrocyte rheology.

Stuart¹

1985

Journal of Clinical Pathology

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SUMMARY Erythrocyte deformability was formerly measured by its contribution to whole blood viscosity. It is now more commonly measured by filtration of erythrocytes through, or aspiration into, pores of 3-5 ,um diameter and by the measurement of shear induced erythrocyte elongation using laser diffractometry. Recent improvements in the technology for erythrocyte filtration have included the removal of acute phase reactants from test erythrocyte suspensions, ultrasonic cleaning and reuse of filter membranes, awareness of the importance of mean cell volume as a determinant of flow through 3 gm diameter pores, and the ability to detect subpopulations of less deformable erythrocytes. Measurements of erythrocyte elongation by laser diffractometry, using the Ektacytometer, are also influenced by cell size and need to be corrected for mean cell volume. These advances have greatly improved the sensitivity and specificity of rheological methods for measuring the deformability of erythrocytes and for investigating the mode of action of rheologically active drugs.An earlier review of blood rheology (the science of the deformation and flow of blood) was mainly concerned with the clinical value of measuring whole blood viscosity.' Five years later, rheological measurements in clinical studies are more often concerned with the individual components of blood (erythrocytes, leucocytes, platelets, and plasma) that collectively influence whole blood viscosity. It is therefore appropriate to review the residual role of measurements of whole blood viscosity while concentrating on new developments in the study of erythrocyte rheology. Measurement of whole blood viscosityThis measurement still has a role as a global test, but its relatively poor sensitivity and specificity, and overdependence on the packed cell volume, have limited its clinical usefulness. Within the normal range there is a linear relation between packed cell volume and the logarithm of whole blood viscosity, but attempts to correct viscosity to a standard packed cell volume of 0-45, using regression lines, are prone to error.2 Regression lines derived from normal blood may not apply to patients' samples,

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Thermal transitions of red blood cell deformability. Correlation with membrane rheological properties

Cited by 22 publications

References 49 publications

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Red Blood Cell Deformability in Sepsis

Erythrocyte rheology.

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