Thermal tolerance role of novel polyamine, caldopentamine, identified in fifth instar Bombyx mori

Lima, Anugata; Didugu, Brinda Goda Lakshmi; Chunduri, Alekhya Rani; Rajan, R. K.; Jha, Anjali; Mamillapalli, Anitha

doi:10.1007/s00726-022-03226-5

Cited by 4 publications

(9 citation statements)

References 70 publications

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“…Total PAs was estimated according to a published protocol with some modifications (Lima et al 2023 ). 100 µl of centrifuged saliva sample was used to estimate PA.…”

Section: Methodsmentioning

confidence: 99%

Combined assessment of lysine and N-acetyl cadaverine levels assist as a potential biomarker of the smoker periodontitis

Rashid,

Yellarthi,

Yellarthi

et al. 2024

Amino Acids

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Periodontitis is an inflammatory condition of supporting structures of teeth leading to attachment and bone loss. Cigarette smoking is the single most important and modifiable risk factor with 5 to 20-fold susceptibility for periodontal diseases. Reverse smoking is a peculiar habit of smoking where the lit end is kept inside the mouth, which is predominant in the northern coastal districts of Andhra Pradesh. Polyamines are biologically active amines involved in tissue regeneration and modulation of inflammation. The study aimed to evaluate polyamines and check their utility as a marker in detection of periodontitis among different groups. Total polyamine levels showed significant increase in reverse smokers with periodontitis when compared to the other groups. Qualitative analysis by thin layer chromatography showed three polyamine bands with varying intensity among the different groups. Mass spectrometric and NMR analyses of the three bands identified them as N1, N8-diacetyl spermidine, N-acetyl cadaverine and lysine. Most significantly elevated levels of lysine was observed in the smoker and reverse smoker periodontitis groups when compared to healthy and non-smoker periodontitis groups. The significantly elevated levels of N-acetyl cadaverine could be responsible for the more destruction of periodontium in the reverse smoker group. Antioxidant potential decreased significantly in different smoker periodontitis groups. The present study suggests that the quantitative analysis of salivary polyamines, lysine and N-acetyl cadaverine can aid as an easy noninvasive diagnostic method for assessing the periodontal status, especially in smokers.

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“…Total PAs was estimated according to a published protocol with some modifications (Lima et al 2023 ). 100 µl of centrifuged saliva sample was used to estimate PA.…”

Section: Methodsmentioning

confidence: 99%

Combined assessment of lysine and N-acetyl cadaverine levels assist as a potential biomarker of the smoker periodontitis

Rashid,

Yellarthi,

Yellarthi

et al. 2024

Amino Acids

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show abstract

“…PA quanti cation was carried out following the extraction of dansylated PAs into the organic phase. A uorescence spectrometer was used to measure PAs (Lima et al, 2023;(Liu et al, 2016). Spd was used as a control, and the results were expressed as micrograms of PAs present in 200 µl of saliva.…”

Section: Estimation Of Pamentioning

confidence: 99%

“…TLC was used to separate the extracted dansylated PAs from the saliva sample according to an earlier published protocol (Lima et al, 2023). A pre-coated silica gel TLC aluminium sheet (60 F254 Merck Millipore) (20 x 10 cm) was used.…”

Section: Tlc Analysismentioning

confidence: 99%

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Combined assessment of Lysine and N-acetyl cadaverine levels assist as a potential biomarker of the smoker periodontitis

Rashid,

Kumar,

kumar

et al. 2024

Preprint

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Periodontitis is an inflammatory condition of supporting structures of teeth leading to attachment and bone loss. Cigarette smoking is the single most important and modifiable risk factor with 5 to 20-fold susceptibility for periodontal diseases. Reverse smoking is a peculiar habit of smoking where the lit end is kept inside the mouth, which is predominant in the northern coastal districts of Andhra Pradesh. Polyamines are biologically active amines involved in tissue regeneration and modulation of inflammation. The study aimed to evaluate polyamines and check their utility as a marker in detection of periodontitis among different groups. Total Polyamine levels showed significant increase in reverse smokers with periodontitis when compared to the other groups. Qualitative analysis by thin layer chromatography showed three polyamine bands with varying intensity among the different groups. Mass spectrometric and NMR analyses of the three bands identified them as N1, N8- diacetyl spermidine, N-acetyl cadaverine and lysine. Most significantly elevated levels of lysine was observed in the smoker and reverese smoker periodontitis groups when compared to healthy and non-smoker periodontitis groups. The significanlty elevated levels of N-acetyl cadaverine could be responsible for the more destruction of periodontium in the reverse smoker group. Antioxidant potential decreased significantly in different smoker periodontitis groups. The present study suggests that the quantitative analysis of salivary polyamines, lysine and N-acetyl cadaverine can aid as an easy noninvasive diagnostic method for assessing the periodontal status, especially in smokers.

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“…The survival of species is negatively impacted by the increased frequency and intensity of high‐temperature episodes resulting from increased global warming (Perkins et al., 2012; Collins et al., 2013; Lima et al., 2023). Temperature affects the living state, distribution, growth, development, and survival of organisms.…”

Section: Introductionmentioning

confidence: 99%

Effects of different diets on thermal tolerance in the silkworm, Bombyx mori

Wang,

Zhang,

Tian

et al. 2023

Entomologia Exp Applicata

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The survival of species is negatively impacted by the increased frequency and intensity of high‐temperature episodes caused by increased global warming. Thermal tolerance can influence population size and the geographic distribution of species, which translates into species‐wide responses to climate and affects interactions between communities of species. The silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), is an ectotherm and an internationally recognized model insect for studying sensitivity to the environment. In the present study, we investigated the effects of different diets on the thermal tolerance of silkworm. As such, it was identified that silkworms reared on an artificial diet in instars I–III and mulberry leaves in instar IV (referred as the AM group) exhibited faster weight gain and higher antioxidant enzyme activity at 35 °C, whereas silkworms reared only on mulberry leaves (referred as the ML group) exhibited upregulated transcript levels of key genes regulating antioxidant enzyme signaling pathways including Akt, CncC, and keap1, significantly increased reactive oxygen species (ROS) levels, and severely impaired midgut activity at 35 °C. Thus, the AM group presented stronger thermal tolerance. These findings suggest that diet can affect the thermal tolerance of silkworm. This research improved our understanding of the molecular, biochemical, and physiological changes caused by thermal stress and laid the groundwork for future research regarding the mechanism underlying thermal tolerance. Furthermore, this study provides an important reference for the conservation of biodiversity in the context of global warming.

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Thermal tolerance role of novel polyamine, caldopentamine, identified in fifth instar Bombyx mori

Cited by 4 publications

References 70 publications

Combined assessment of lysine and N-acetyl cadaverine levels assist as a potential biomarker of the smoker periodontitis

Combined assessment of lysine and N-acetyl cadaverine levels assist as a potential biomarker of the smoker periodontitis

Combined assessment of Lysine and N-acetyl cadaverine levels assist as a potential biomarker of the smoker periodontitis

Effects of different diets on thermal tolerance in the silkworm, Bombyx mori

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