Thermal tolerance and acclimation capacity in the European common frog (<i>Rana temporaria</i>) change throughout ontogeny

Ruthsatz, Katharina; Dausmann, Kathrin H.; Peck, Myron A.; Glos, Julian

doi:10.1002/jez.2582

Cited by 30 publications

(52 citation statements)

References 113 publications

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“…In order to evaluate whether acclimation effects differ among populations, we used a two‐way analysis of variance of CT max and CT min , with temperature (6, 13, 20, and 27°C) and population as fixed factors. We estimated the Acclimation Response Ratio (ARR) that measures the change in thermal tolerance relative to change in acclimation temperature (Claussen, 1977 ; Ruthsatz et al, 2022 ). In the case of ARR for upper thermal tolerance, ARR = [(highest CT max – lowest CT max ) /Δ°C].…”

Section: Methodsmentioning

confidence: 99%

Phenology and plasticity can prevent adaptive clines in thermal tolerance across temperate mountains: The importance of the elevation‐time axis

Gutiérrez‐Pesquera

Tejedo

Camacho

et al. 2022

Ecology and Evolution

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Critical thermal limits (CT max and CT min ) decrease with elevation, with greater change in CT min , and the risk to suffer heat and cold stress increasing at the gradient ends. A central prediction is that populations will adapt to the prevailing climatic conditions. Yet, reliable support for such expectation is scant because of the complexity of integrating phenotypic, molecular divergence and organism exposure. We examined intraspecific variation of CT max and CT min , neutral variation for 11 microsatellite loci, and micro‐ and macro‐temperatures in larvae from 11 populations of the Galician common frog ( Rana parvipalmata ) across an elevational gradient, to assess (1) the existence of local adaptation through a P ST ‐F ST comparison, (2) the acclimation scope in both thermal limits, and (3) the vulnerability to suffer acute heat and cold thermal stress, measured at both macro‐ and microclimatic scales. Our study revealed significant microgeographic variation in CT max and CT min , and unexpected elevation gradients in pond temperatures. However, variation in CT max and CT min could not be attributed to selection because critical thermal limits were not correlated to elevation or temperatures. Differences in breeding phenology among populations resulted in exposure to higher and more variable temperatures at mid and high elevations. Accordingly, mid‐ and high‐elevation populations had higher CT max and CT min plasticities than lowland populations, but not more extreme CT max and CT min . Thus, our results support the prediction that plasticity and phenological shifts may hinder local adaptation, promoting thermal niche conservatism. This may simply be a consequence of a coupled variation of reproductive timing with elevation (the “elevation‐time axis” for temperature variation). Mid and high mountain populations of R. parvipalmata are more vulnerable to heat and cool impacts than lowland populations during the aquatic phase. All of this contradicts some of the existing predictions on adaptive thermal clines and vulnerability to climate change in elevational gradients.

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Section: Methodsmentioning

confidence: 99%

Phenology and plasticity can prevent adaptive clines in thermal tolerance across temperate mountains: The importance of the elevation‐time axis

Gutiérrez‐Pesquera

Tejedo

Camacho

et al. 2022

Ecology and Evolution

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“…However, within a given family or genus, the evolution of CTMax seems to be more related to the microhabitat occupied by species themselves, as suggested by literature for amphibians and lizards (Duarte et al, 2012;Gunderson et al, 2018;Carilo Filho et al, 2021). In amphibian's ontogeny, CTMax increases throughout larval development and usually decreases during the metamorphic climax-morphophysiological transition that occurs between the larval and adult stages (Floyd, 1983;Ruthsatz et al, 2022). Once the metamorphosis phase is over, the thermal tolerance values tend to increase again (Bodensteiner et al, 2021;Ruthsatz et al, 2022) but there are evidences that larval forms tend to have a CTMax greater than the adults for tropical species from Atlantic Forest (Carilo Filho et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…In amphibian's ontogeny, CTMax increases throughout larval development and usually decreases during the metamorphic climax-morphophysiological transition that occurs between the larval and adult stages (Floyd, 1983;Ruthsatz et al, 2022). Once the metamorphosis phase is over, the thermal tolerance values tend to increase again (Bodensteiner et al, 2021;Ruthsatz et al, 2022) but there are evidences that larval forms tend to have a CTMax greater than the adults for tropical species from Atlantic Forest (Carilo Filho et al, 2021). If the data in amphibians are not yet conclusive, in squamate reptiles the pattern is even less consistent (see Bodensteiner et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

There and back again: A meta-analytical approach on the influence of acclimation and altitude in the upper thermal tolerance of amphibians and reptiles

et al. 2022

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Realistic predictions about the impacts of climate change onbiodiversity requires gathering ecophysiological data and the critical thermal maxima (CTMax) is the most frequently used index to assess the thermal vulnerability of species. In the present study, we performed a systematic review to understand how acclimation and altitude affect CTMax estimates for amphibian and non-avian reptile species. We retrieved CTMax data for anurans, salamanders, lizards, snakes, and turtles/terrapins. Data allowed to perform a multilevel random effects meta-analysis to answer how acclimation temperature affect CTMax of Anura, Caudata, and Squamata and also meta-regressions to assess the influence of altitude on CTMax of frogs and lizards. Acclimation temperature influenced CTMax estimates of tadpoles, adult anurans, salamanders, and lizards, but not of froglets. In general, the increase in acclimation temperature led to higher CTMax values. Altitudinal bioclimatic gradient had an inverse effect for estimating the CTMax of lizards and anuran amphibians. For lizards, CTMax was positively influenced by the mean temperature of the wettest quarter. For anurans, the relationship is inverse; we recover a trend of decreasing CTMax when max temperature of warmest month and precipitation seasonality increase. There is an urgent need for studies to investigate the thermal tolerance of subsampled groups or even for which we do not have any information such as Gymnophiona, Serpentes, Amphisbaena, and Testudines. Broader phylogenetic coverage is mandatory for more accurate analyses of macroecological and evolutionary patterns for thermal tolerance indices as CTMax.

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“…However, the tolerance–plasticity trade-off hypothesis (van Heerwaarden and Kellermann, 2020) proposes that basal heat tolerance and thermal plasticity are negatively correlated; such that individuals with high CT max have limited hardening (Gilbert and Miles, 2019). While numerous studies have demonstrated that amphibians exhibit plastic basal heat tolerance (e.g., Cupp Jr, 1980; Ruthsatz et al, 2022), hardening remains understudied.…”

Section: Discussionmentioning

confidence: 99%

“…These tests were conducted on larval wood frogs, Lithobates sylvaticus (LeConte 1825). Because larval anurans display a positive relationship between acclimation temperature and CT max (e.g., Cupp Jr, 1980; Ruthsatz et al, 2022), we predicted that longer acclimation to warmer temperatures would increase basal heat tolerance compared to those acclimated to cooler temperatures. In line with the trade-off hypothesis, we also expected the hardening effect would be most pronounced in larvae with the lowest CT max suggesting greater acute thermal plasticity under these environments.…”

Section: Introductionmentioning

confidence: 99%

Heat Hardening of a Larval Amphibian is Dependent on Acclimation Period and Temperature

Dallas

Warne²

2022

Preprint

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The thermal tolerance–plasticity trade-off hypothesis states that acclimation to warmer environments increases basal thermal tolerance in ectotherms but reduces plasticity in coping with acute thermal stress characterized as heat hardening. We examined the potential trade-off between basal heat tolerance and hardening plasticity, measured as critical thermal maximum (CTmax) of a larval amphibian, Lithobates sylvaticus, in response to differing acclimation temperatures (15° and 25°C) and periods (3 or 7 days). A hardening treatment applied 2 hours before CTmax assays induced pronounced plastic hardening responses in the cool, 15°C treatment after 7 days of acclimation, compared to controls. Warm acclimated larvae at 25°C, by contrast, exhibited minor hardening responses, but significantly increased basal thermal tolerance. These results support the trade-off hypothesis and fill a knowledge gap in larval amphibian thermal plasticity. Elevated environmental temperatures induce acclimation in heat tolerance yet constrains ectotherm capacity to cope with further acute thermal stress.

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Thermal tolerance and acclimation capacity in the European common frog (Rana temporaria) change throughout ontogeny

Cited by 30 publications

References 113 publications

Phenology and plasticity can prevent adaptive clines in thermal tolerance across temperate mountains: The importance of the elevation‐time axis

Phenology and plasticity can prevent adaptive clines in thermal tolerance across temperate mountains: The importance of the elevation‐time axis

There and back again: A meta-analytical approach on the influence of acclimation and altitude in the upper thermal tolerance of amphibians and reptiles

Heat Hardening of a Larval Amphibian is Dependent on Acclimation Period and Temperature

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