Thermal Therapy in Urologic Systems: A Comparison of Arrhenius and Thermal Isoeffective Dose Models in Predicting Hyperthermic Injury

He, Xiaoming; Bhowmick, Sankha; Bischof, John C.

doi:10.1115/1.3128671

Cited by 56 publications

(53 citation statements)

References 38 publications

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“…The activation energy and frequency factor can be determined from the slope and intercept of the linear fit, respectively. [7, 15, 32] This procedure involves tedious experiments under many different conditions over several orders of magnitude in heating time. With the controlled heating rate method proposed, it is possible to obtain the kinetic parameters from fewer experiments (3 runs, instead of 5 × 3 runs).…”

Section: Discussionmentioning

confidence: 99%

Correlated Parameter Fit of Arrhenius Model for Thermal Denaturation of Proteins and Cells

et al. 2014

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Thermal denaturation of proteins is critical to cell injury, food science and other biomaterial processing. For example protein denaturation correlates strongly with cell death by heating, and is increasingly of interest in focal thermal therapies of cancer and other diseases at temperatures which often exceed 50 °C. The Arrhenius model is a simple yet widely used model for both protein denaturation and cell injury. To establish the utility of the Arrhenius model for protein denaturation at 50 °C and above its sensitivities to the kinetic parameters (activation energy Ea and frequency factor A) were carefully examined. We propose a simplified correlated parameter fit to the Arrhenius model by treating Ea, as an independent fitting parameter and allowing A to follow dependently. The utility of the correlated parameter fit is demonstrated on thermal denaturation of proteins and cells from the literature as a validation, and new experimental measurements in our lab using FTIR spectroscopy to demonstrate broad applicability of this method. Finally, we demonstrate that the end-temperature within which the denaturation is measured is important and changes the kinetics. Specifically, higher Ea and A parameters were found at low end-temperature (50°C) and reduce as end-temperatures increase to 70 °C. This trend is consistent with Arrhenius parameters for cell injury in the literature that are significantly higher for clonogenics (45 – 50 °C) vs. membrane dye assays (60 –70 °C). Future opportunities to monitor cell injury by spectroscopic measurement of protein denaturation are discussed.

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Section: Discussionmentioning

confidence: 99%

Correlated Parameter Fit of Arrhenius Model for Thermal Denaturation of Proteins and Cells

et al. 2014

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“…Errors may arise when the value used was obtained from experiments with a much different temperature range or heating mechanism (e.g., heating rate, intensity). For example, the time required for tissue coagulation in thermal therapy (> 50 °C) may be less than the critical CEM 43 obtained from experiments in the hyperthermia temperature range (40 – 45 °C) [41]. To make use of the simplicity of the thermal dose model and to ensure prediction accuracy, a chart of applicable critical CEM 43 in various different ranges of temperature could be further established using the same experimental set-up and processing method used in this study with varying HIFU intensities and exposure durations.…”

Section: Resultsmentioning

confidence: 99%

Characterization of lesion formation and bubble activities during high-intensity focused ultrasound ablation using temperature-derived parameters

Hsiao

Kumon

Deng

2013

Infrared Physics & Technology

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Successful high-intensity focused ultrasound (HIFU) thermal tissue ablation relies on accurate information of the tissue temperature and tissue status. Often temperature measurements are used to predict and monitor the ablation process. In this study, we conducted HIFU ablation experiments with ex vivo porcine myocardium tissue specimens to identify changes in temperature associated with tissue coagulation and bubble/cavity formation. Using infrared (IR) thermography and synchronized bright-field imaging with HIFU applied near the tissue surface, parameters derived from the spatiotemporal evolution of temperature were correlated with HIFU-induced lesion formation and overheating, of which the latter typically results in cavity generation and/or tissue dehydration. Emissivity of porcine myocardium was first measured to be 0.857 ± 0.006 (n = 3). HIFU outcomes were classified into non-ablative, normal lesion, and overheated lesion. A marked increase in the rate of temperature change during HIFU application was observed with lesion formation. A criterion using the maximum normalized second time derivative of temperature change provided 99.1% accuracy for lesion identification with a 0.05 s−1 threshold. Asymmetric temperature distribution on the tissue surface was observed to correlate with overheating and/or bubble generation. A criterion using the maximum displacement of the spatial location of the peak temperature provided 90.9% accuracy to identify overheated lesion with a 0.16 mm threshold. Spatiotemporal evolution of temperature obtained using IR imaging allowed determination of the cumulative equivalent minutes at 43 °C (CEM43) for lesion formation to be 170 min. Similar temperature characteristics indicative of lesion formation and overheating were identified for subsurface HIFU ablation. These results suggest that parameters derived from temperature changes during HIFU application are associated with irreversible changes in tissue and may provide useful information for monitoring HIFU treatment.

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“…Another type of thermal therapy, called thermo-ablation, destroys the tumor cells directly via cell necrosis, coagulation or carbonization by exposing them to temperatures ranging from 46 to 56°C in short intervals (seconds to a few minutes) (Nayak et al 1977;Jordan et al 1999;Lui et al 2003). The kinetics of thermal injury is a key factor for estimating cell survivability, and the kinetics of different cell lines has been addressed (Diller and Ryan 1998;He et al 2009). Some surgical techniques of thermal injury to kill malignant cells/tissue apply a peak temperature ranging from 50 to 70°C for several seconds to several minutes (Diller and Ryan 1998).…”

Section: Introductionmentioning

confidence: 99%

Single-cell-based measurement of supraphysiological thermal injury in human carcinoma cells utilizing a micropatterned hydrogel chip

2010

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Hyperthermia affects certain regulatory proteins, kinases or cyclins, resulting in alternations to the cell cycle and even to apoptosis. Damage to the cell plasma membrane is a key factor in the killing of a cell by hyperthermia. Analysis at the single-cell level is necessary for understanding the fundamental mechanisms of hyperthermia-induced cell death and the generation of thermotolerance in surviving cells. Engineering approaches achieving precise control of cellular micropatterning provide the potential for investigating the mechanisms of thermal injury to cells at the single-cell level. The main purpose of this study is to fabricate a hydrogel chip with microwells for cellular patterning and to demonstrate the feasibility of measurement of supraphysiological thermal injury in human carcinoma cells (HeLa cells) at the singlecell level. To accomplish this, measurement of membrane injury by dye leakage post-thermal insult was performed and reported in this work. A hydrogel chip with microwells with different diameters was fabricated. For cell concentrations at 0.5 9 10 6 cells/mL, the occupancy of cells on the microchip with 40 lm microwells was up to 86.6%, a value far higher than that found on the 30 lm microwells (approximately 78.5%). Most microwells of 30 lm in diameter (about 70%) were occupied by a single cell; hence, the hydrogel chip with 30 lm microwells was suitable for the applications of single-cell-based analysis. The fluorescent images showed that calcein leakage occurred when cell membranes were damaged under supraphysiological temperatures between 43 and 50°C. The normalized intensity of calcein decreased to 32% under a supraphysiological temperature of 43°C for 20 min. The intensity of calcein in cells was less than 20% under a supraphysiological temperature of 50°C. The feasibility of the single-cell-based experiment of thermal injury in the microchip with hydrogel microwells was therefore successfully demonstrated.

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Thermal Therapy in Urologic Systems: A Comparison of Arrhenius and Thermal Isoeffective Dose Models in Predicting Hyperthermic Injury

Cited by 56 publications

References 38 publications

Correlated Parameter Fit of Arrhenius Model for Thermal Denaturation of Proteins and Cells

Correlated Parameter Fit of Arrhenius Model for Thermal Denaturation of Proteins and Cells

Characterization of lesion formation and bubble activities during high-intensity focused ultrasound ablation using temperature-derived parameters

Single-cell-based measurement of supraphysiological thermal injury in human carcinoma cells utilizing a micropatterned hydrogel chip

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