Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization

Wu, Youbin; Wei, Wei; Zhou, Meng; Wang, Yueqi; Wu, Jie; Ma, Guanghui; Su, Zhiguo

doi:10.1016/j.biomaterials.2011.11.068

Cited by 96 publications

(70 citation statements)

References 31 publications

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“…In particular, microand nano-particle delivery systems that encapsulate protein antigens or DNA that encode for antigens have been evaluated. However, the focus of intranasal vaccination has often been on the treatment of infectious diseases 29,[41][42][43][44][45][46][47] . Nonetheless, a recent study has demonstrated that nanoparticles composed of modified c-polyglutamic acid (c-PGA) encapsulating full length OVA protein instilled intranasally induced anti-tumor immunity against melanoma 48 .…”

Section: Discussionmentioning

confidence: 99%

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Phua

Staats

Nair

et al. 2015

Journal of Controlled Release

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Section: Discussionmentioning

confidence: 99%

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Phua

Staats

Nair

et al. 2015

Journal of Controlled Release

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“…Antigen-specific IgG (total, IgG, IgG1, and IgG2a) in serum and secretory IgA (sIgA) in mucosal wash (nasal, tracheal, and lung wash) were measured by enzyme-linked immunosorbent assay (ELISA), as described previously (18). Briefly, ELISA plates were coated overnight at 4°C with 2 g H9N2 WIV/well and then were blocked by incubation with 1% (wt/vol) bovine serum albumin (BSA) in PBS containing 0.05% Tween (PBST) for 1 h at 37°C.…”

Section: Influenza Whole Inactivated Virus (Wiv) Is More Immunogenic mentioning

confidence: 99%

H9N2 Influenza Whole Inactivated Virus Combined with Polyethyleneimine Strongly Enhances Mucosal and Systemic Immunity after Intranasal Immunization in Mice

Qin¹,

Yin²,

Huang³

et al. 2015

Clin. Vaccine Immunol.

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Influenza whole inactivated virus (WIV) is more immunogenic and induces protective antibody responses compared with otherformulations, like split virus or subunit vaccines, after intranasal mucosal delivery. Polyethyleneimine (PEI), an organic polycation, is widely used as a reagent for gene transfection and DNA vaccine delivery. Although PEI recently has demonstrated potent mucosal adjuvant activity for viral subunit glycoprotein antigens, its immune activity with H9N2 WIV is not well demonstrated. Here, mice were immunized intranasally with H9N2 WIV combined with PEI, and the levels of local respiratory tract and systemic immune responses were measured. Compared to H9N2 WIV alone, antigen-specific IgA levels in the local nasal cavity, trachea, and lung, as well as levels of IgG and its subtypes (IgG1 and IgG2a) in the serum, were strongly enhanced with the combination. Similarly, the activation and proliferation of splenocytes were markedly increased. In addition, PEI is superior as an H9N2 WIV delivery system due to its ability to greatly increase the viral adhesion to mucosal epithelial cells and to enhance the cellular uptake and endosomal escape of antigens in dendritic cells (DCs) and further significantly activate DCs to mature. Taken together, these results provided more insights that PEI has potential as an adjuvant for H9N2 particle antigen intranasal vaccination.T he rise and spread of the low-pathogenic avian H9N2 influenza virus have seriously increased the risk of a new influenza pandemic. H9N2 viruses have prevailed in chickens in China in recent years and have constantly undergone reassortment, and novel genotypes have continued to emerge (1-3). A novel H7N9 reassortant subtype was recently found to cause severe human respiratory infections in China (4). Bioinformatic analyses for the H7N9 virus revealed that its six internal genes were from H9N2 avian influenza viruses of chickens (5). Thus, the elimination of low-pathogenic avian H9N2 influenza virus in poultry becomes even more important in influenza prevention.The nasal cavity of the respiratory tract is the primary entry site of the H9N2 influenza virus, and the viral infection could be discontinued if intranasal immunity is well established (6). Compared with live attenuated influenza vaccines or subunit influenza vaccines (such as purified viral hemagglutinin [HA]) or neuraminidase [NA]) proteins), whole inactivated H9N2 influenza vaccines have more advantages, including an improved safety profile, higher immunogenicity, more effective ability of establishing cross-protection at the pathogen's entry site, and stronger crosspresentation of antigens by dendritic cells (DCs) for a CD8 ϩ T cell response against viruses (7-9). However, mucosal immunization by intranasal delivery with inactivated virus alone is often poorly effective. Unlike systemic immunization, nasal antigens must cross various barriers (compact epithelium, mucociliary clearance, and mucus) before they contact with submucosal immune cells (10). Many researchers used various ...

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“…Hydrogels may be prepared so that their physical-chemical characteristics (e.g., equilibrium swelling and absorption kinetic) respond to changes in their external environment, such as temperature [3], pH [4,5], ionic strength [6], and so on. These materials show a wide variety of applications such as drug delivery [7], membranes for (bio)separation process [8], substrate for cell culture [9], and mucosal vaccines [10].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of a pH-responsive poly(ethylene glycol)-based hydrogel: acid degradation, equilibrium swelling, and absorption kinetic characteristics

et al. 2015

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We developed a pH-responsive, hydrogel based on poly(ethylene glycol) (PEG) covalently cross-linked with acrylic acid and N′,N′-dimethylacrylamide along with acidlabile groups. In the hydrogel, PEG plays a role as the key constituent. If its chains break, the polymer networks are destroyed. The pharmacological potential of these hydrogels were demonstrated by determining their water transport profile, modulus of elasticity, and cytotoxicity assay. The hydrogels showed a pseudo-Fickian behavior, a transport mechanism that occurs when the diffusion coefficient changes with the time and the swelling equilibrium is never fully reached. At pH 2, the PEG-richer hydrogels degraded and the scanning electron microscopy (SEM) images illustrated less-defined shapes than at pH 7 and 10. This morphological characteristic results of the hydrogel deconstruction owing to cleavage of ether bonds of the PEG chains unmaking its 3D polymer network. The proposed hydrogels were shown to be compatible to cells, indicating acceptable biocompatibility and an appropriate level of security for use in the biological environments. Furthermore, they showed structural changes in their polymer network in response to pH, which is an important characteristic for stimuli-triggered release of guest molecules.

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Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization

Cited by 96 publications

References 31 publications

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

H9N2 Influenza Whole Inactivated Virus Combined with Polyethyleneimine Strongly Enhances Mucosal and Systemic Immunity after Intranasal Immunization in Mice

Synthesis and characterization of a pH-responsive poly(ethylene glycol)-based hydrogel: acid degradation, equilibrium swelling, and absorption kinetic characteristics

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