Thermal Reversal Surface with “Sticky Tentacle” for Modulating Initial Cell Adhesion and Detachment

Li, Na; Wang, Yangang; Zhao, Dan; Deng, Bo; Fan, Xialian; He, Xichan

doi:10.1016/j.matdes.2020.109402

Cited by 9 publications

(2 citation statements)

References 44 publications

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“…Comparatively more hydrophilic polymers that contain polar functional groups provide surface hydration through strong hydrogen bonding with water molecules in aqueous solution. Therefore, a water hydration barrier formed between the polymer and hydrogen molecules could determine the conformation and type of essential proteins adsorbed on the polymer surface and subsequently affect the firmness of cell attachment (Chen et al, 2018; De los Santos Pereira et al, 2016; Leng et al, 2015; Wei et al, 2015). As a result, PNIPAm‐ co ‐AAm‐ b ‐PS (5%) provided maximum cell detachment and was chosen to coat cytodex 3 microcarriers for further 3D cell culturing and thermal‐induced cell detachment from the microcarriers.…”

Section: Resultsmentioning

confidence: 99%

Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing

Darge

Chuang

Lai

et al. 2021

Biotech & Bioengineering

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Enzymatic detachment of cells might damage important features and functions of cells and could affect subsequent cell‐based applications. Therefore, nonenzymatic cell detachment using thermosensitive polymer matrix is necessary for maintaining cell quality after harvesting. In this study, we prepared thermosensitive PNIPAm‐co‐AAc‐b‐PS and PNIPAm‐co‐AAm‐b‐PS copolymers and low critical solution temperature (LCST) was tuned near to body temperature. Then, spin coated polymer films were prepared for cell adhesion and thermal‐induced cell detachment. The alpha‐step analysis and scanning electron microscope image of the films suggested that the thickness of the films depends on the molecular weight and concentration which ranged from 206 to 1330 nm for PNIPAm‐co‐AAc‐b‐PS and 97.5–497 nm for PNIPAm‐co‐AAm‐b‐PS. The contact angles of the films verified that the polymer surface was moderately hydrophilic at 37°C. Importantly, RAW264.7 cells were convincingly proliferated on the films to a confluent of >80% within 48 h and abled to detach by reducing the temperature. However, relatively more cells were grown on PNIPAm‐co‐AAm‐b‐PS (5%w/v) films and thermal‐induced cell detachment was more abundant in this formulation. As a result, PNIPAm‐co‐AAm‐b‐PS (5%w/v) was further used to coat commercial cytodex 3 microcarriers for 3D cell culturing and interestingly enhanced cell detachment with preserved potential of recovery was observed at a temperature of below LCST. Thus, surface modification of microcarriers with thermosensitive PNIPAm‐co‐AAm‐b‐PS could be vital strategy for nonenzymatic cell detachment and to achieve adequate number of cells with maximum cell viability and functionality.

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Section: Resultsmentioning

confidence: 99%

Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing

Darge

Chuang

Lai

et al. 2021

Biotech & Bioengineering

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“…Therefore, an unanswered question is how to develop fast, reliable, and label-free methods for cell characterization and identification that do not require (bio)-chemical markers or receptors. To this end, opportunities may lie in the knowledge of how cells respond to various stimuli and the resulting morphological and motility processes that occur either at the whole-cell level, e.g., cell detachment, migration, oscillations, [11][12][13] or at the intracellular level, such as the spatiotemporal changes in protein complexes and metabolites. [14,15] Thermal stimuli, including local heating, and temperature gradients across cells have a strong potential for inducing morphological and cytoskeletal changes: examples include the formation of membrane extensions, [16] redistribution of actomyosin complexes, [17] tubulin, and actin polymerization [18,19] through symmetry breaking.…”

Section: Introductionmentioning

confidence: 99%

Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

et al. 2022

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Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label-free methods without (bio)-chemical markers or surface-engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label-and receptor-free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell-detachment with sharply defined time-patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell-type-specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic-type oscillations are observed during detachment of mammalian and yeast-cell ensembles, providing additional cell-specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs.

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Photothermal scaffolds/surfaces for regulation of cell behaviors

Zheng

et al. 2022

Bioactive Materials

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Thermal Reversal Surface with “Sticky Tentacle” for Modulating Initial Cell Adhesion and Detachment

Cited by 9 publications

References 44 publications

Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing

Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing

Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

Photothermal scaffolds/surfaces for regulation of cell behaviors

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