Thermal responsive poly-N-isopropylacrylamide/galactomannan copolymer nanoparticles as a potential amphotericin delivery carrier

Lima, Laís Ramos Monteiro de; Cavalcante, Clara M.W.S. A; Carneiro, Maria J.M.; Mendes, Josilayne F. S.; Sousa, Nayara Alves de; Freire, Raquel Santiago; Pinto, V. P. T.; Fontenelle, Raquel Oliveira dos Santos; Feitosa, Judith P.A.; Paula, Regina C.M. de

doi:10.1016/j.carpta.2021.100126

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…Moreover, an additional vibration band arose at 1381 cm −1 for the alkyl C-O-C modes for the epoxy groups, indicating the presence of GMA segments in the pNIPAAm-co-GMA copolymer samples. Additionally, after the Lys units were modified, new peaks formed at 1552 cm −1 and 1632 cm −1 signifying amine (-NH), carboxylic acid groups, indicating the existence of functionalized Lys units in the pNIPAAm-co-GMA HG sample [32]. Moreover, the FITC conjugation was verified by the appearance of peaks at 1456 cm −1 and 1725 cm −1 , which indicate the imine (-C=N) and -C=S groups of the FITC molecules, respectively (Figure 1C).…”

Section: Characterization Of Pnipaam-co-gma and Pnipaam-co-gma-lys Hg...mentioning

confidence: 99%

L-Lysine-Modified pNIPAm-co-GMA Copolymer Hydrogel for pH- and Temperature-Responsive Drug Delivery and Fluorescence Imaging Applications

Moorthy

Vanaraj

Thirupathi³

et al. 2023

Gels

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The development of dual-stimuli-responsive hydrogels attracts much research interest owing to its unique stimuli-responsive characteristics. In this study, a poly-N-isopropyl acrylamide-co-glycidyl methacrylate-based copolymer was synthesized by incorporating N-isopropyl acrylamide (NIPAm) and a glycidyl methacrylate (GMA) monomer. The synthesized copolymer, pNIPAm-co-GMA was further modified with L-lysine (Lys) functional units and further conjugated with fluorescent isothiocyanate (FITC) to produce a fluorescent copolymer pNIPAAm-co-GMA-Lys hydrogel (HG). The in vitro drug loading and dual pH- and temperature-stimuli-responsive drug release behavior of the pNIPAAm-co-GMA-Lys HG was investigated at different pH (pH 7.4, 6.2, and 4.0) and temperature (25 °C, 37 °C, and 45 °C) conditions, respectively, using curcumin (Cur) as a model anticancer drug. The Cur drug-loaded pNIPAAm-co-GMA-Lys/Cur HG showed a relatively slow drug release behavior at a physiological pH (pH 7.4) and low temperature (25 °C) condition, whereas enhanced drug release was achieved at acidic pH (pH 6.2 and 4.0) and higher temperature (37 °C and 45 °C) conditions. Furthermore, the in vitro biocompatibility and intracellular fluorescence imaging were examined using the MDA-MB-231 cell line. Therefore, we demonstrate that the synthesized pNIPAAm-co-GMA-Lys HG system with temperature- and pH-stimuli-responsive features could be promising for various applications in biomedical fields, including drug delivery, gene delivery, tissue engineering, diagnosis, antibacterial/antifouling material, and implantable devices.

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Section: Characterization Of Pnipaam-co-gma and Pnipaam-co-gma-lys Hg...mentioning

confidence: 99%

L-Lysine-Modified pNIPAm-co-GMA Copolymer Hydrogel for pH- and Temperature-Responsive Drug Delivery and Fluorescence Imaging Applications

Moorthy

Vanaraj

Thirupathi³

et al. 2023

Gels

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“…Polymer grafting or graft copolymerization is a facile method to introduce hydrophobic alkyl chains to the alginate backbone. Poly(methyl methacrylate) (PMMA), poly(acrylonitrile) (PAN), PNIPAM (Poly( N -isopropylacrylamide) , are commonly used polymers that impart amphiphilic properties to alginate . Redox, electron beam, microwave irradiation and gamma ray irradiation are used for grafting of alginates while redox remains the most common method .…”

Section: Introductionmentioning

confidence: 99%

“…Poly(methyl methacrylate) (PMMA), 60 poly(acrylonitrile) (PAN), 4 PNIPAM (Poly( N -isopropylacrylamide) 61 , 62 are commonly used polymers that impart amphiphilic properties to alginate. 63 Redox, electron beam, microwave irradiation and gamma ray irradiation are used for grafting of alginates while redox remains the most common method. 64 For the free radical graft polymerization, an initiator is added for the polymerization to occur, and the pyran ring of the alginate might open in the course of the reaction.…”

Section: Introductionmentioning

confidence: 99%

A Review on Hydrophobically Associated Alginates: Approaches and Applications

Akshaya,

Nathanael

2024

ACS Omega

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Alginates are linear anionic polysaccharides, which are well-known for their biocompatible, nontoxic, and biodegradable nature. The polymer consists of alternating units of β-(1 → 4)-linked D-mannuronic acid (M) and α-(1 → 4)-linked L-guluronic acid (G) that have hydroxyl and carboxyl groups as the main functional groups. As a large number of free carboxyl and hydroxyl groups are present in the polymeric chain, the polymer is predominantly hydrophilic. The food and pharmaceutical industries have been the most extensive utilizers of alginates to produce gelling and thickening agents. However, by imparting hydrophobicity to alginates, the range of applications can be widened. Although there are reviews on alginate and its chemical modifications, reviews focusing on hydrophobically associated alginates have not been presented. The commonly used chemical modifications to incorporate hydrophobicity include esterification, Ugi reaction, reductive amination, and graft copolymerization. The hydrophobically modified alginates play an important role in delivery of hydrophobic drugs and pesticides as the modification increases the affinity toward hydrophobic components and helps in their sustained release. Due to their nontoxic and edible nature, they find use in the food industry as emulsion stabilizer to stabilize oil-in-water emulsions and to improve creaming ability. Further, alginate-based materials such as membranes, aerogels, and films are hydrophobically modified to improve their functionality and applicability to water treatment and food packaging. This Review aims to highlight the important chemical modifications and methods that are done to impart hydrophobicity to alginate, and the applications of hydrophobically modified alginates in different sectors ranging from drug delivery to food packaging are discussed.

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Study of thermal transitions of copolymers based on N‐isopropylacrylamide and acrylic acid with potential for curcumin controlled release

Ramírez‐Irigoyen

García-Verdugo

Castillo‐Ortega

et al. 2023

J of Applied Polymer Sci

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Thermosensitive polymers based on poly(N‐isopropylacrylamide) (pNIPAM) have been widely evaluated in a variety of biomedical applications due to their particular thermal behavior in aqueous solutions. Despite this, few works have focused on the complementary analysis of the thermal transitions of pNIPAM polymers in linear and crosslinked form. In this work, linear and crosslinked poly(N‐isopropylacrylamide‐co‐acrylic acid) p(NIPAM‐co‐AA) copolymers were synthesized at similar NIPAM/AA feed composition and their thermosensitive behavior was studied by turbidimetric methods, FTIR spectroscopy analysis, and temperature‐dependent swelling measurements. The intermolecular crosslinking hindered the hydrophobic aggregation of chain segments, leading to higher transition temperatures of synthesized polymers. AA units promoted the intersegment hydrogen bonds during heating and strengthened the hydrogen bond interactions water‐network. The effect of the thermosensitive behavior of p(NIPAM‐co‐AA) copolymer on curcumin (CUR) release kinetic was also studied. The low uptake level of p(NIPAM‐co‐AA) hydrogel, partially shrunken at 37°C, produced a CUR sustained delivery, reaching the release equilibrium state up to 18 h. Hydrogels of p(NIPAM‐co‐AA) with a suitable composition exhibited a promising performance for the CUR controlled delivery at physiological conditions.

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Thermal responsive poly-N-isopropylacrylamide/galactomannan copolymer nanoparticles as a potential amphotericin delivery carrier

Cited by 6 publications

References 45 publications

L-Lysine-Modified pNIPAm-co-GMA Copolymer Hydrogel for pH- and Temperature-Responsive Drug Delivery and Fluorescence Imaging Applications

L-Lysine-Modified pNIPAm-co-GMA Copolymer Hydrogel for pH- and Temperature-Responsive Drug Delivery and Fluorescence Imaging Applications

A Review on Hydrophobically Associated Alginates: Approaches and Applications

Study of thermal transitions of copolymers based on N‐isopropylacrylamide and acrylic acid with potential for curcumin controlled release

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