Thermal Inactivation Study of Partially Purified PPO from Algerian Dates (Phoenix dactylifera L cv Tedala ) using Pyrogallol as Substrate

Abstract

This study investigated the stability and thermal inactivation of Tedala PPO using pyrogallol as substrate in range of temperature between 60 and 75°C.The kinetic of Tedala PPO inactivation by temperature followed a biphasic model .The D values during thermal inactivation varied from 1771,21 to 1,7 min for Tedala PPO and the corresponding Z values and activation energy (Ea) were 6,6 °C and 334,3 kJ/mol and 4,8°C and 458,7kJ/mol for both heat sensitive and heat stable fractions ,respectively. Likewise To understand therefore the behavior of enzyme with increase of temperature,the thermodynamic study was carried out and their parameters (ΔH, ΔG and ΔS) were calculated.