Thermal inactivation of shrimp deoxyribonuclease with and without sodium dodecyl sulfate

Lin, Jinn-Nan; Wang, Wenyi; Liao, Ta–Hsiu

doi:10.1016/0167-4838(94)90186-4

Cited by 7 publications

(9 citation statements)

References 17 publications

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“…The Western blot method limits the analysis of Ab response to proteins having a sufficiently high threshold of expression. Furthermore, native conformational epitopes could disappear during the electrophoresis steps, even if partial refolding of proteins is possible (49,50). This could explain a partial loss of IgG reactivity, and the emergence of a restricted repertoire against self-Ags in sera of both healthy subjects and MS patients.…”

Section: Discussionmentioning

confidence: 99%

Distortion of the Self-Reactive IgG Antibody Repertoire in Multiple Sclerosis as a New Diagnostic Tool

Lefranc¹,

Almeras²,

Dubucquoi³

et al. 2004

The Journal of Immunology

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To date, none of the myelin-associated Ag targets definitively discriminates between the immune response observed in multiple sclerosis (MS) patients and healthy subjects. However, it has been shown recently that analysis of global immune Ab profiles such as natural autoantibody reactivities can help to distinguish between normal individuals and patients suffering from various immune diseases. The aim of our study was to compare the global IgG immune response against brain self-Ags in sera from 82 MS patients and 27 healthy subjects. The analysis of the immune profiles was performed by Western blotting, and data were subjected to linear discriminant analysis. Particular patterns of IgG reactivity were found in healthy subjects, Sjögren patients, and MS patients. Moreover, this approach separated the three clinical forms of MS with a high concordance rate with the clinical data (κ value, 77.8%). Our study suggests, for the first time, that serum IgG Ab repertoires are able to distinguish MS patients. In addition, our data suggest that patterns of IgG reactivity could model the pathological processes underlying the various forms of MS. Further characterization of such discriminant Ags could provide useful information regarding their potent role in pathogenesis or regulatory processes in MS.

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Section: Discussionmentioning

confidence: 99%

Distortion of the Self-Reactive IgG Antibody Repertoire in Multiple Sclerosis as a New Diagnostic Tool

Lefranc¹,

Almeras²,

Dubucquoi³

et al. 2004

The Journal of Immunology

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“…However, the Form II′ could not be converted to Form II, even after treatment with SDS (reaction d). Based on these observations the authors opined that the irreversible denaturation on heating in the absence of SDS is due to the formation of the incorrect inside‐out structure, with an exposed hydrophobic domain which has a tendency to aggregate and cannot revert to a catalytically active form [100].…”

Section: Physical Propertiesmentioning

confidence: 99%

Sugar non-specific endonucleases

Rangarajan

Shankar

2001

FEMS Microbiol Rev

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Sugar non-specific endonucleases are multifunctional enzymes and are widespread in distribution. Apart from nutrition, they have also been implicated in cellular functions like replication, recombination and repair. Their ability to recognize different DNA structures has also been exploited for the determination of nucleic acid structure. Although more than 30 non-specific endonucleases have been isolated to date, very little information is available regarding their structure-function correlations except that of staphylococcal and Serratia nucleases. However, during the past few years, the primary structure, nature of the active site based on sequence homology, and the probable mechanism of action have been postulated for some of the enzymes. This review describes the purification, characteristics, biological role and applications of sugar non-specific endonucleases.

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“…However, the Form IIP could not be converted to Form II, even after treatment with SDS (reaction d). Based on these observations the authors opined that the irreversible denaturation on heating in the absence of SDS is due to the formation of the incorrect inside-out structure, with an exposed hydrophobic domain which has a tendency to aggregate and cannot revert to a catalytically active form [100].…”

Section: Puri¢cationmentioning

confidence: 99%

Sugar non-specific endonucleases

Rangarajan¹

2001

FEMS Microbiology Reviews

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Sugar non-specific endonucleases are multifunctional enzymes and are widespread in distribution. Apart from nutrition, they have also been implicated in cellular functions like replication, recombination and repair. Their ability to recognize different DNA structures has also been exploited for the determination of nucleic acid structure. Although more than 30 non-specific endonucleases have been isolated to date, very little information is available regarding their structure^function correlations except that of staphylococcal and Serratia nucleases. However, during the past few years, the primary structure, nature of the active site based on sequence homology, and the probable mechanism of action have been postulated for some of the enzymes. This review describes the purification, characteristics, biological role and applications of sugar non-specific endonucleases. ß

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Thermal inactivation of shrimp deoxyribonuclease with and without sodium dodecyl sulfate

Cited by 7 publications

References 17 publications

Distortion of the Self-Reactive IgG Antibody Repertoire in Multiple Sclerosis as a New Diagnostic Tool

Distortion of the Self-Reactive IgG Antibody Repertoire in Multiple Sclerosis as a New Diagnostic Tool

Sugar non-specific endonucleases

Sugar non-specific endonucleases

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