Thermal effects of percutaneous application of plasma/radiofrequency energy on porcine dermis and fibroseptal network

Ruff, Paul G.

doi:10.1111/jocd.13845

Cited by 8 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Renuvion (Apyx Medical Corporation, Clearwater, Florida), a helium-based radiofrequency device that combines radiofrequency (rf) energy and helium plasma, may be another approach to stimulate the tissue by combining their two effects: promotion of angiogenesis through heating the tissue, and the proliferation of ASCs and paracrine recruitment of immune cells in response to plasma treatment [ 21 – 25 ]. Furthermore, plasma treatment in mouse wound models was shown to augment tissue oxygenation [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Ruff,

Sterodimas

2023

Aesth Plast Surg

Self Cite

View full text Add to dashboard Cite

Background Improvements to autologous fat grafting for soft tissue augmentation are needed to overcome the unpredictable volume retention. Approaches such as fat harvesting and processing, injection technique, preparation of the recipient site, and supplemental biologics are topics of ongoing research. Here, an energy-based device was investigated as a stimulatory tool for recipient site preparation for improving fat graft retention. Objective The objective was to measure the stimulatory responses in fat grafts after 4 weeks when using a helium-based radiofrequency device to pretreat the recipient tissue. Methods Using an autologous fat grafting mouse model, the inguinal fat pad was grafted in a small cranial pocket after either a saline injection alone (control) or a saline injection followed by pretreatment (treated). The fat pad was resected after 4 weeks, sectioned and stained with immunofluorescence markers to investigate tissue remodeling. Results Pretreatment resulted in higher viability of adipocytes, a higher concentration of viable ASCs in areas of adipose tissue regeneration, and localized macrophages in the areas of regeneration when compared to the control. There was no observable difference in vascularity or angiogenesis. The staining for ASCs was higher in the pretreated group in comparison with the control group (5.0% vs. 3.3%, p=0.36) when using a pixel classifier in QuPath in the viable adipose tissue regions. Conclusions The use of a helium-based radiofrequency device as a pretreatment tool appears to increase the viability of the adipose tissue likely due to higher concentration of ASCs. The apparent increase in viable ASCs may be due to enhanced proliferation or paracrine recruitment of these cells in response to the helium-based radiofrequency treatment. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Bullet List of Important Points: Pretreatment of the fat graft recipient site increases the viability of the adipose tissue after 4 weeks in comparison with the control grafts. The increased viability is likely due to the observed increase in adipose-derived stem cells in the pretreated group. Pretreatment enhanced the adipose tissue remodeling as colocalization of adipose-derived stem cells and macrophages showed an active remodeling, whereas the control group exhibited more necrotic and fibrotic tissue.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Ruff,

Sterodimas

2023

Aesth Plast Surg

Self Cite

View full text Add to dashboard Cite

show abstract

“…Low-temperature plasma radiofrequency ablation have been studied for the treatment of tonsillectomy, removal of nasopharyngeal carcinoma, mixed hemorrhoids, and sinusitis. [27][28][29] However, there are few reports on the treatment of pterygium with low-temperature plasma radiofrequency ablation. We hope to combine the autologous conjunctival transplantation and low-temperature plasma radiofrequency ablation to treat pterygium more effectively.…”

Section: Observation Of Clinical Indicatorsmentioning

confidence: 99%

Low-temperature plasma radiofrequency ablation and autologous conjunctival transplantation for pterygium excision.

Song

Jin

Zhu

et al. 2022

Preprint

View full text Add to dashboard Cite

Background/Aims: To evaluate the safety and effectiveness of low-temperature plasma radiofrequency ablation combined with autologous conjunctival transplantation for pterygium excision. Methods: In order to verify the safety of low-temperature plasma radiofrequency ablation, we conducted animal experiments to observe corneal structure by anterior segment photography, anterior optical coherence tomography, HE staining and immunohistochemistry(MMP-9,TNF-a) before and after corneal burning at different ablation energies (20, 15, 10, 0J/cm2)and different point in time(0,3d,1w,2w). Then, we observed preoperative and postoperative treatment effect of 121 eyes of 120 patients with primary pterygium from June 2018 to June 2020. 59 patients treated with pterygium excision and autologous conjunctival transplantation, 61 patients treated with pterygium excision by low-temperature plasma radiofrequency ablation and autologous conjunctival transplantation. The time to strip the pterygium, the integrity of the cornea and the number of inflammatory cell infiltration were observed and recorded. All patients were followed up for 18 months to observe whether the pterygium recurred. Results: The animal experiment confirmed that suitable low-temperature plasma radiofrequency ablation energies has no damage to the corneal tissue structure. Only one case recurred during the year of follow-up. Low-temperature plasma radiofrequency ablation can reduce the time to strip the pterygium, can better maintain the integrity of the damaged cornea and reduce the number of inflammatory cell infiltration, can reduce recurrence rate. Conclusions: Low-temperature plasma radiofrequency ablation combined with autologous conjunctival transplantation is an safety and effective pterygium excision.

show abstract

New Technologies in Skin Tightening

Malone¹,

Walters

Stroh

et al. 2021

Curr Otorhinolaryngol Rep

View full text Add to dashboard Cite

Thermal effects of percutaneous application of plasma/radiofrequency energy on porcine dermis and fibroseptal network

Cited by 8 publications

References 19 publications

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Low-temperature plasma radiofrequency ablation and autologous conjunctival transplantation for pterygium excision.

New Technologies in Skin Tightening

Contact Info

Product

Resources

About