Thermal denaturation of recombinant human lysozyme from rice: effect of pH and comparison with human milk lysozyme

Castillo, Eduardo; Franco, Indira; Pérez, María Dolores; Calvo, Miguel; Sánchez, Lourdes

doi:10.1007/s00217-011-1612-8

Cited by 6 publications

(1 citation statement)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration of lactoperoxidase was measured according ISO/TS 17193:2011. The concentration of lysozyme was analyzed using the spectrophotometric method of enzymatic activity of lysozyme EC 3.2.1.17 (Sigma-Aldrich, Buchs, Switzerland) with some modifications according Castillo et al [25]. Lactose was determined using enzymatic method by commercial kit MEGAZYME (Megazyme, Bray, Ireland).…”

Section: Determination Of Gross Composition and Bioactive Compoundsmentioning

confidence: 99%

Fermentation Ability of Bovine Colostrum by Different Probiotic Strains

Hyršlová¹,

Krausová²,

Michlová

et al. 2020

Fermentation

View full text Add to dashboard Cite

Over the past decade, the use of bovine colostrum and its bioactive components as the basis of functional food and dietary supplements for humans has substantially increased. However, for developing new products enriched with probiotics and bovine colostrum, the influence of colostrum composition on the growth promotion of bacteria still needs to be tested. Therefore, we decided to study the influence of bovine colostrum chemical and mineral composition as well as the content of bioactive compounds (immunoglobulins, lactoferrin, lactoperoxidase) on the growth of ten selected strains from genera Lactobacillus, Lacticaseibacillus, Bifidobacterium, and Enterococcus. After 24 h of fermentation, the growth was assessed based on lactic and acetic acids production evaluated using isotachophoresis, bacterial counts determined by the agar plate method, and change of pH. The production of acids and bacterial counts were significantly (P<0.05) different between selected genera. The change of bacterial counts was correlated with pH, but the correlation between growth and bovine colostrum composition was not proven. The highest growth and production of lactic acid was observed after the fermentation of bovine colostrum by the strains Enterococcus faecium CCDM 922A and CCDM 945.

show abstract

Section: Determination Of Gross Composition and Bioactive Compoundsmentioning

confidence: 99%