Thermal cycling effect on osteogenic differentiation of MC3T3-E1 cells loaded on 3D-porous Biphasic Calcium Phosphate (BCP) scaffolds for early osteogenesis

Sayed, Shithima; Faruq, Omar; Hossain, Monir; Im, Soo Bin; Kim, Yong-Sik; Lee, Byong-Taek

doi:10.1016/j.msec.2019.110027

Cited by 23 publications

(18 citation statements)

References 45 publications

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“…Among the main osteogenic marks, during the early period of osteogenesis, the improvement of ALP activity is a vital event occurring [ 42 ]. In the study, for the ALP activity of cells on the specimens, both PT20 and PT40 containing Ta particles revealed higher ALP activity than PEKK.…”

Section: Discussionmentioning

confidence: 99%

Implantable PEKK/tantalum microparticles composite with improved surface performances for regulating cell behaviors, promoting bone formation and osseointegration

Mei

Qian

et al. 2021

Bioactive Materials

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Polyetherketoneketone (PEKK) exhibits admirable biocompatibility and mechanical performances but bioinert while tantalum (Ta) possesses excellent osteogenesis and osseointegration but high elastic modulus and density, and processing is too difficult and expensive. In the present study, combining of the advantages of both PEKK and Ta, implantable composites of PEKK/Ta were fabricated by blending PEKK with Ta microparticles of 20 v% (PT20) and 40 v% (PT40) content. In comparison with PT20 and PEKK, the surface hydrophilicity, surface energy, roughness and proteins adsorption as well as mechanical performances of PT40 significantly increased because of the higher Ta particles content in PEKK. Furthermore, PT40 exhibited the mechanical performances (e.g., compressive strength and modulus of elasticity) close to the cortical bone of human. Compared with PT20 and PEKK, PT40 with higher Ta content remarkably enhanced the responses (including adhesion, proliferation and osteogenic differentiation) of MC3T3-E1 cells in vitro . Moreover, PT40 markedly improved bone formation as well as osseointegration in vivo . In short, incorporation of Ta microparticles into PEKK created implantable composites with improved surface performances, which played key roles in stimulating cell responses/bone formation as well as promoting osseointegration. PT40 might have great potential for bear-loading bone substitute.

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Section: Discussionmentioning

confidence: 99%

Implantable PEKK/tantalum microparticles composite with improved surface performances for regulating cell behaviors, promoting bone formation and osseointegration

Mei

Qian

et al. 2021

Bioactive Materials

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“…Low mechanical strength is not conducive to bearing compression and pull from external forces, and a fast degradation rate goes against cell differentiation and cartilage repair. [13][14][15][16][17][18] Moreover, the hydrophilic collagen scaffolds have difficulty loading hydrophobic drugs uniformly, which limits the application of collagen scaffold materials in drug loading and sustained release. 19,20 In addition, currently most of the prepared collagen scaffolds consist of singlepore structures, which do not satisfy the pore requirements for the multi-scale porous structures of cartilage.…”

Section: Introductionmentioning

confidence: 99%

Sodium alginate/collagen composite multiscale porous scaffolds containing poly(ε-caprolactone) microspheres fabricated based on additive manufacturing technology

Liu

Huang

et al. 2020

RSC Adv.

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“…In our study, we identified the interaction between RUNX2 and HSP70 in present of overexpression of TRIM16. In previous studies, HSP70 also plays an important role in cell differentiation and tissue development, including in osteogenic differentiation (Loones and Morange, 1998;Lui and Kong, 2007;Sayed et al, 2019). HSP70 knockdown impairs osteogenic and chondrogenic differentiation in human mesenchymal stem cells .…”

Section: Discussionmentioning

confidence: 91%

TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2

Zhao

Zhai

Liu

et al. 2021

Front. Cell Dev. Biol.

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Bone regeneration is the ultimate goal of periodontal therapies, in which osteogenic differentiation of human periodontal ligament stem cells plays a critical role. The tripartite motif (TRIM)16, an E3 ubiquitin ligase, is downregulated in periodontal tissues of patients with periodontitis, while the role of TRIM16 in the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) is largely unknown. Firstly, we found that TRIM16 was increased throughout the osteogenic media induced differentiation of hPDLSCs. Then overexpression plasmids and specific short-hairpin RNAs (shRNAs) were constructed to manipulate the expression of target molecules. TRIM16 significantly promoted alkaline phosphatase activity, mineralized nodule formation, and positively regulated the expression of osteo-specific markers RUNX2, COL1A1 and OCN except the mRNA of RUNX2. Mechanistically, TRIM16 serves as a pivotal factor that stabilizes RUNX2 protein levels by decreasing CHIP-mediated K48-linked ubiquitination degradation of the RUNX2 protein. This study identified a novel mechanism of TRIM16 in regulating stability of the RUNX2 protein, which promoted the osteogenic differentiation of hPDLSCs. TRIM16 may be a potential target of stem cell based-bone regeneration for periodontal therapies.

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Thermal cycling effect on osteogenic differentiation of MC3T3-E1 cells loaded on 3D-porous Biphasic Calcium Phosphate (BCP) scaffolds for early osteogenesis

Cited by 23 publications

References 45 publications

Implantable PEKK/tantalum microparticles composite with improved surface performances for regulating cell behaviors, promoting bone formation and osseointegration

Implantable PEKK/tantalum microparticles composite with improved surface performances for regulating cell behaviors, promoting bone formation and osseointegration

Sodium alginate/collagen composite multiscale porous scaffolds containing poly(ε-caprolactone) microspheres fabricated based on additive manufacturing technology

TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2

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