Thermal conditions experienced during differentiation affect metabolic and contractile phenotypes of mouse myotubes

Little, Alexander G.; Seebacher, Frank

doi:10.1152/ajpregu.00148.2016

Cited by 12 publications

(11 citation statements)

References 35 publications

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“…The complexity of cellular and molecular adaptation to cold stress has been poorly investigated. Exposure of C2C12 myoblasts to low temperature was previously found to increase the metabolic flux, and differentiating myotubes respond to low temperature by increasing their metabolic rate, ATP production, and glycolytic flux [23]. These findings are in line with the results of our experiments on myoblasts cultured for 72 h. We found that the rate of basal respiration, ATP production, and glycolysis was significantly increased at low temperature.…”

Section: Discussionsupporting

confidence: 92%

Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts

Sajjanar

Siengdee

Trakooljul

et al. 2019

International Journal of Hyperthermia

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Objective: Environmental stress induces disturbances in cell energy metabolism and may cause epigenetic modifications. This study aimed to understand the possible impact of temperature stress (35 C, 39 C and 41 C, compared to control 37 C) on energy metabolism and epigenetic modifications, such as DNA methylation and histone H4 acetylation, as well as its effects on the expression of genes responsible for epigenetic changes, in mouse skeletal myoblasts (C2C12 cells). Methods: The results showed significantly reduced maximal respiration and spare respiratory capacity under heat stress (39 C and 41 C), suggesting that mitochondrial functions were compromised under these conditions. The glycolytic capacity and glycolysis markedly increased following low-temperature stress (35 C). The results suggested that, under cold stress, cells prefer glycolysis as a rapid compensatory mechanism to meet energy requirements for adaptive thermogenic response. Results: Epigenetic changes (histone H4 acetylation and global DNA methylation) were observed under both heat and cold stress. Among the genes coding for DNA methyltransferases, the Dnmt3a was significantly increased under high-temperature conditions (39 C and 41 C), while Dnmt1 expression was significantly increased at low temperature (35 C), indicating that under these conditions the cells preferred maintenance of methylation to de novo methylation activity. An expression pattern similar to Dnmt3a was observed for Gcn5, encoding for a histone acetyltransferase. The study revealed that temperature stress induced changes in the metabolic profiles, as well as epigenetic modifications, including the dynamics of the key enzymes. Conclusion: The results indicated the existence of crosstalk mechanisms between energy metabolism and epigenetics during cell stress response. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 92%

Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts

Sajjanar

Siengdee

Trakooljul

et al. 2019

International Journal of Hyperthermia

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“…In cold-exposed BAT, for instance, TRPM8 enhanced Ca 2+ -influx and increased the expression of UCP1 independently from the canonical β-adrenergic pathway (Ma et al, 2012 ). Our results, showing that mild hypothermia (32°C) altered metabolic phenotypes of myoblasts and subsequent myotubes (Little and Seebacher, 2016 ), are especially interesting because brown adipocytes and skeletal myocytes both share a myf-5 positive mesenchymal stem cell origin (Seale et al, 2008 ), which means that TRPM8 activation may also underlie metabolic programming to compensate for mild hypothermia in skeletal muscle development, repair, and maintenance.…”

Section: Mechanisms Mediating Plasticitymentioning

confidence: 76%

“…In mouse muscle, the capacity to shift performance curves in response to temperature is independent from central neuroendocrine input and can occur in isolated cells (Little and Seebacher, 2016 ). Cool (32°C) growth temperature of muscle precursor cells (myoblasts) lowered the mode of the thermal performance curve for metabolic rate compared to control (37°C) conditions, where increased metabolic rate at 32°C compensated for the negative thermodynamic effects.…”

Section: Plasticity Of Performance Curves In Response To Tissue Tempementioning

confidence: 99%

“…Similarly, decreased differentiation temperature (32°C) of myoblasts into functional myotubes lowered the mode of the thermal performance curve for metabolic rate, again compensating for cool temperatures. Interestingly, myoblast growth temperature influenced myotube thermal performance independently from differentiation temperature (Little and Seebacher, 2016 ). These thermal responses of mouse myocytes are comparable to the interaction between developmental and reversible plasticity in ectotherms (Scott and Johnston, 2012 ; Little et al, 2013 ; Beaman et al, 2016 ).…”

Section: Plasticity Of Performance Curves In Response To Tissue Tempementioning

confidence: 99%

“…Plasticity of thermal performance curves may be regulated centrally via sympathetic output from the hypothalamus (Nakamura and Morrison, 2007 ; Seebacher, 2009 ), and peripherally by circulating levels of thyroid hormone (Little and Seebacher, 2013 , 2014 ; Little et al, 2013 ). However, the capacity to adjust thermal sensitivity in response to local changes in temperature can also occur independently from neuroendocrine input (Al-Fageeh et al, 2006 ; Underhill and Smales, 2007 ; Ye et al, 2013 ; Little and Seebacher, 2016 ). For example, changes in membrane fluidity resulting from temperature-sensitive shifts in fatty acid profiles can maintain cellular, organelle, and protein function in variable thermal environments (Cossins and Prosser, 1978 ).…”

Section: Mechanisms Mediating Plasticitymentioning

confidence: 99%

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Plasticity of Performance Curves Can Buffer Reaction Rates from Body Temperature Variation in Active Endotherms

Seebacher

Little

2017

Front. Physiol.

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Endotherms regulate their core body temperature by adjusting metabolic heat production and insulation. Endothermic body temperatures are therefore relatively stable compared to external temperatures. The thermal sensitivity of biochemical reaction rates is thought to have co-evolved with body temperature regulation so that optimal reaction rates occur at the regulated body temperature. However, recent data show that core body temperatures even of non-torpid endotherms fluctuate considerably. Additionally, peripheral temperatures can be considerably lower and more variable than core body temperatures. Here we discuss whether published data support the hypothesis that thermal performance curves of physiological reaction rates are plastic so that performance is maintained despite variable body temperatures within active (non-torpid) endotherms, and we explore mechanisms that confer plasticity. There is evidence that thermal performance curves in tissues that experience thermal fluctuations can be plastic, although this question remains relatively unexplored for endotherms. Mechanisms that alter thermal responses locally at the tissue level include transient potential receptor ion channels (TRPV and TRPM) and the AMP-activated protein kinase (AMPK) both of which can influence metabolism and energy expenditure. Additionally, the thermal sensitivity of processes that cause post-transcriptional RNA degradation can promote the relative expression of cold-responsive genes. Endotherms can respond to environmental fluctuations similarly to ectotherms, and thermal plasticity complements core body temperature regulation to increase whole-organism performance. Thermal plasticity is ancestral to endothermic thermoregulation, but it has not lost its selective advantage so that modern endotherms are a physiological composite of ancestral ectothermic and derived endothermic traits.

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Metabolic cold adaptation in the Asiatic toad: intraspecific comparison along an altitudinal gradient

Tan

Yao

et al. 2021

J Comp Physiol B

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Thermal conditions experienced during differentiation affect metabolic and contractile phenotypes of mouse myotubes

Abstract: Little AG, Seebacher F. Thermal conditions experienced during differentiation affect metabolic and contractile phenotypes of mouse myotubes.

Cited by 12 publications

References 35 publications

Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts

Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts

Plasticity of Performance Curves Can Buffer Reaction Rates from Body Temperature Variation in Active Endotherms

Metabolic cold adaptation in the Asiatic toad: intraspecific comparison along an altitudinal gradient

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