Thermal and chemical resistance of Lactobacillus casei and Lactobacillus paracasei bacteriophages

Capra, María Luján; Quiberoni, Andrea del Luján; Reinheimer, Jorge Alberto

doi:10.1111/j.1472-765x.2004.01525.x

Cited by 85 publications

(103 citation statements)

References 29 publications

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“…Some phages are highly resistant to sodium hypochlorite at the concentrations used in the food industry, and this disinfectant does not eliminate or reduce the numbers of most bacteriophages (42). Alcoholic solutions, such as 70% ethanol, have been reported to be unable to harm most bacteriophages (42,43). It appears that only peracetic acid is a reliable disinfectant for most bacteriophages (42), but at present, this is not extensively used for disinfection.…”

Section: Discussionmentioning

confidence: 99%

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Bacteriophages Isolated from Chicken Meat and the Horizontal Transfer of Antimicrobial Resistance Genes

Shousha

Awaiwanont

Sofka

et al. 2015

Appl Environ Microbiol

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c Antimicrobial resistance in microbes poses a global and increasing threat to public health. The horizontal transfer of antimicrobial resistance genes was thought to be due largely to conjugative plasmids or transposons, with only a minor part being played by transduction through bacteriophages. However, whole-genome sequencing has recently shown that the latter mechanism could be highly important in the exchange of antimicrobial resistance genes between microorganisms and environments. The transfer of antimicrobial resistance genes by phages could underlie the origin of resistant bacteria found in food. We show that chicken meat carries a number of phages capable of transferring antimicrobial resistance. Of 243 phages randomly isolated from chicken meat, about a quarter (24.7%) were able to transduce resistance to one or more of the five antimicrobials tested into Escherichia coli ATCC 13706 (DSM 12242). Resistance to kanamycin was transduced the most often, followed by that to chloramphenicol, with four phages transducing tetracycline resistance and three transducing ampicillin resistance. Phages able to transduce antimicrobial resistance were isolated from 44% of the samples of chicken meat that we tested. The statistically significant (P ‫؍‬ 0.01) relationship between the presence of phages transducing kanamycin resistance and E. coli isolates resistant to this antibiotic suggests that transduction may be an important mechanism for transferring kanamycin resistance to E. coli. It appears that the transduction of resistance to certain antimicrobials, e.g., kanamycin, not only is widely distributed in E. coli isolates found on meat but also could represent a major mechanism for resistance transfer. The result is of high importance for animal and human health.

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Section: Discussionmentioning

confidence: 99%

“…Phages differ from their bacterial hosts in a number of important properties, such as their ability to persist in the presence of disinfectants (42,43). Thus, disinfectants used to kill bacteria are, in many cases, not able to eliminate bacteriophages.…”

Section: Discussionmentioning

confidence: 99%

Bacteriophages Isolated from Chicken Meat and the Horizontal Transfer of Antimicrobial Resistance Genes

Shousha

Awaiwanont

Sofka

et al. 2015

Appl Environ Microbiol

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“…Resistance of EEP to different temperatures, ethanol levels and pH values was determined, according to the methods of Capra et al [14] . All results were expressed as the concentration (PFU/ml) of active viral particles and its log 10 plotted against time.…”

Section: Physicochemical Stability Of Eepmentioning

confidence: 99%

Characterization and Genome Sequencing of a Novel Coliphage Isolated from Engineered <i>Escherichia coli</i>

Liu

Zhu

et al. 2010

Intervirology

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Objectives: To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP). Methods and Results: Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) and a long, flexible tail (138 nm in length). EEP was able to infect all 10 engineered E. coli strains kept in our laboratory, showing a strong ability to lyse engineered E. coli. Sequencing of the EEP genome revealed a double-stranded DNA (39.8 kb) with 54.72% GC content. Fifty-two open reading frames were predicted to be coding sequences, 18 of which were functionally defined and organized in a modular format, which includes modules for DNA replication, DNA packaging, structural proteins and host cell lysis. This phage could not be inactivated at 90° for 45 min and was resistant to ethanol and alkali treatment. EEP is assigned to the Siphoviridae family based on its morphological, genomic and physicochemical properties. Conclusions: A novel coliphage was isolated from engineered E. coli strains, and its morphological, genomic and physicochemical properties were characterized, which will improve our knowledge of bacteriophage diversity.

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“…After treatment, the soft-agar overlay method was used to determine surviving phages at different temperatures or pH [5].…”

Section: Thermal Stability and Ph Sensitivitymentioning

confidence: 99%

Isolation and Complete Genome Sequence of a Novel Pseudoalteromonas Phage PH357 from the Yangtze River Estuary

et al. 2017

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Phage PH357, a novel lytic Pseudoalteromonas lipolytica phage belonging to the Myoviridae family was isolated from the Yangtze River estuary. The microbiological characterization demonstrated that phage PH357 is stable from -20 to 60 °C and the optimal pH 7. The one-step growth curve showed a latent period of 20 min, a rise period of 20 min, and the average burst size was about 85 virions per cell. Complete genome of phage PH357 was determined. Genome of phage PH357 consisted of a linear, double-stranded 136,203 bp DNA molecule with 34.58% G + C content, and 242 putative open reading frames (ORFs) without tRNA. All the predicted ORFs were classified into eight functional groups, including DNA replication, regulation and nucleotide metabolism, transcription, translation, phage packaging, phage structure, lysis, host or phage interactions, and hypothetical protein. A phylogenetic analysis showed that phage PH357 had similarity to the previously published Pseudoalteromonas phage PH101 and Vibrio phages. Furthermore, the study of phage PH357 genome will provide useful information for further research on the interaction between phages and their hosts.

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Thermal and chemical resistance of Lactobacillus casei and Lactobacillus paracasei bacteriophages

Cited by 85 publications

References 29 publications

Bacteriophages Isolated from Chicken Meat and the Horizontal Transfer of Antimicrobial Resistance Genes

Bacteriophages Isolated from Chicken Meat and the Horizontal Transfer of Antimicrobial Resistance Genes

Characterization and Genome Sequencing of a Novel Coliphage Isolated from Engineered <i>Escherichia coli</i>

Isolation and Complete Genome Sequence of a Novel Pseudoalteromonas Phage PH357 from the Yangtze River Estuary

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