Thermal Aggregation of Bovine Serum Albumin in Conventional Buffers: An Insight into Molecular Level Interactions

Satish, Lakkoji; Millan, Sabera; Das, Satyabrata; Jena, Subrat Kumar; Sahoo, Harekrushna

doi:10.1007/s10953-017-0612-0

Cited by 22 publications

(9 citation statements)

References 29 publications

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“…To summarize, in view of the current picture that the buffer influences the protein structure and stability by binding to its surface [16,17,18], the glycine buffer at low pH, where the HEWL molecule is highly positively charged has, itself in the cationic form, the lowest tendency to bind to the protein surface. This is reflected in destabilization of the protein structure upon increasing the ionic strength, as well as upon increasing temperature.…”

Section: Resultsmentioning

confidence: 99%

“…Interfacial damage caused during sample agitation could also play an important role in fibril formation of HEWL. Small molecules, in this case buffer ions, can bind to the protein surface, and act as a protective coating, stabilizing the native structure of the protein [16,43,56].…”

Section: Discussionmentioning

confidence: 99%

“…It’s been established in the last couple of years that the buffer does not merely maintain the appropriate pH of the solution at which the protein is stable in its native structure but can actually influence the stability of the protein itself. If the buffer molecules preferentially bind to the native state of the protein, this would stabilize the protein, while in the cases where buffer molecules bind selectively to the denatured state, this would lead to destabilization of the protein native structure [16,17,18]. Buffers also influence the hydration shell of protein molecules which has an impact on the protein stability.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism

Brudar

Hribar-Lee

2019

Biomolecules

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Amyloid fibrils, highly ordered protein aggregates, play an important role in the onset of several neurological disorders. Many studies have assessed amyloid fibril formation under specific solution conditions, but they all lack an important phenomena in biological solutions—buffer specific effects. We have focused on the formation of hen egg-white lysozyme (HEWL) fibrils in aqueous solutions of different buffers in both acidic and basic pH range. By means of UV-Vis spectroscopy, fluorescence measurements and CD spectroscopy, we have managed to show that fibrillization of HEWL is affected by buffer identity (glycine, TRIS, phosphate, KCl-HCl, cacodylate, HEPES, acetate), solution pH, sample incubation (agitated vs. static) and added excipients (NaCl and PEG). HEWL only forms amyloid fibrils at pH = 2.0 under agitated conditions in glycine and KCl-HCl buffers of high enough ionic strength. Phosphate buffer on the other hand stabilizes the HEWL molecules. Similar stabilization effect was achieved by addition of PEG12000 molecules to the solution.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism

Brudar

Hribar-Lee

2019

Biomolecules

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“…Some of the popular algorithms for inverting the measured correlation are described in several classical textbooks on the subject 16. Although most inversion techniques are based on Brownian diffusion (low concentration regime), there are some that are developed for gel-like systems26 typical of the high concentration scattering systems, such as the lens. Some arguments have been made to support the use of these models for inverting in vivo correlation data from the lens.…”

Section: Methodsmentioning

confidence: 99%

Observational Clinical Studies of Human Lens Transparency Using the Vision Index Pen

Abazari

Dhadwal

Wittpenn

2019

Trans. Vis. Sci. Tech.

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PurposePreventing or delaying the onset of presbyopia and cataract formation remains a challenge. The goal of this study was to establish the utility of the Vision Index Pen (VIP), designed to measure in vivo dynamic light scattering (DLS) from the crystalline lens, in the detection of early cataract or loss of accommodation and to show reproducibility through trials at two independent sites. The gradual loss of transparency of the lens was characterized by the lens crystallin aggregation index (LCX) derived from measured DLS data.MethodsVolunteers in different age groups participated in two independently operated observational clinical studies. All subjects underwent a detailed eye exam and VIP measurement of the intensity correlation of the backscattered light from the lens.ResultsLCX values extracted from DLS data show strong correlation with the aging lens, ranging from 20 at the age of 20 years to over 150 at 60 years. Quantitatively significant changes in the LCX value occur from 35 years to 55 years. LCX values were found to correlate with the loss of accommodation (correlation of −0.563 with P < 0.001) and with published data regarding the change in relative lens resistance with age.ConclusionsResults from two independent observational clinical trials have confirmed the repeatability and reproducibility of the VIP diagnostic device as a viable clinical tool for tracking localized macromolecular changes taking place in the aging lens. Detection of early changes in the crystalline lens can be useful in developing patient-specific prediction models, which can be used to screen patients who may benefit from early therapeutic interventions for delaying the onset of presbyopia and cataract growth.Translational RelevanceThe VIP diagnostic device provides in vivo access to the human lens, enabling characterization of the unfolding and decomposition of long-lived macromolecules.

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“…Micro-sized meshes can directly be assimilated as an ECM protein network offering a realistic support for cell growth. Hydrogels can be easily obtained by crosslinking a monomer solution either chemically [ 60 ], under radiation [ 61 ], but also by their self-assembling mechanisms [ 62 ] or through an aggregation mechanism after protein denaturation [ 63 ]. One can, for instance, find hydrogels based on alginate [ 64 ], gelatin [ 65 ], hydroxypropylmethyl cellulose [ 66 ], or hybrid combinations of fibrinogen and polyethylene glycol [ 67 ].…”

Section: Overview Of the Techniques Used For Fabrication Of Porousmentioning

confidence: 99%

The Overview of Porous, Bioactive Scaffolds as Instructive Biomaterials for Tissue Regeneration and Their Clinical Translation

2020

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Porous scaffolds have been employed for decades in the biomedical field where researchers have been seeking to produce an environment which could approach one of the extracellular matrixes supporting cells in natural tissues. Such three-dimensional systems offer many degrees of freedom to modulate cell activity, ranging from the chemistry of the structure and the architectural properties such as the porosity, the pore, and interconnection size. All these features can be exploited synergistically to tailor the cell–material interactions, and further, the tissue growth within the voids of the scaffold. Herein, an overview of the materials employed to generate porous scaffolds as well as the various techniques that are used to process them is supplied. Furthermore, scaffold parameters which modulate cell behavior are identified under distinct aspects: the architecture of inert scaffolds (i.e., pore and interconnection size, porosity, mechanical properties, etc.) alone on cell functions followed by comparison with bioactive scaffolds to grasp the most relevant features driving tissue regeneration. Finally, in vivo outcomes are highlighted comparing the accordance between in vitro and in vivo results in order to tackle the future translational challenges in tissue repair and regeneration.

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Thermal Aggregation of Bovine Serum Albumin in Conventional Buffers: An Insight into Molecular Level Interactions

Cited by 22 publications

References 29 publications

The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism

The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism

Observational Clinical Studies of Human Lens Transparency Using the Vision Index Pen

The Overview of Porous, Bioactive Scaffolds as Instructive Biomaterials for Tissue Regeneration and Their Clinical Translation

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