Thermal acclimation, preference and effects on .Vo2 in the earthworm Lumbricus terrestris

Fitzpatrick, Lloyd C.; Goven, Arthur J.; Earle, Brian D.; Rodríguez, Jorge Egocheaga; Briceño, Jorge; Venables, Barney J.

doi:10.1016/0300-9629(87)90029-6

Cited by 14 publications

(3 citation statements)

References 3 publications

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“…Above that temperature range, the induction of Hsp70 decreased and almost completely disappeared when the tissue was incubated at 37ЊC. It is interesting to point out that a heat stress response was not observed at 15ЊC, a temperature reported to be in the ''optimal activity'' range for this earthworm species (Fitzpatrick et al 1987).…”

Section: Heat Shock Treatment Induces Hsp70mentioning

confidence: 91%

Evaluation for Hsp70 as a biomarker of effect of pollutants on the earthworm Lumbricus terrestris

Nadeau

Corneau²,

Plante³

et al. 2001

Cell Stress Chaper

115

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Induction of heat shock proteins (Hsps) is often associated with a cellular response to a harmful stress or to adverse life conditions. The main aims of the present study were (1) to assess if stress-induced Hsp70 could be used to monitor exposure of the earthworm species Lumbricus terrestris to various soil pollutants, (2) to assess the specificity of pollutants in their tissue targeting and in Hsp70 induction, and (3) to evaluate if dose-response relationships could be established and if the stress-response observed was specific. The midgut/intestinal tissues of L. terrestris are shown to express an inducible member of the Hsp70 family after heat shock treatment in vitro and exposures to different soil toxicants in vivo (re: artificial soil). Short-term (24-72 hours) and long-term (14-16 days) exposures to the chemical standards chloroacetamide and pentachlorophenol and to heavy metals (Pb++, Cd++, Cu++, and Hg++) also affected the earthworms, and Hsp70 was induced in their midgut/intestinal tissues. After a 3-day exposure to heavy metals, the level of Hsp70 induction in the midgut/intestinal tissues appears to correlate well with the reported in vivo and in vitro toxicity data. Comparatively, in proximal and midbody wall muscle tissues of animals exposed to the heavy metals, a decrease in expression of Hsp70 was sometimes detected. Thus Hsp analysis by Western blot in L. terrestris tissues and particularly in the midgut/intestine proved to be a suitable and sensitive assay for adverse effects in earthworms and showed a good level of reproducibility despite some individual variations. The use of pristine/nonexposed animals transposed into contaminated environments as in the present study should therefore be of high ecological relevance. Induction of Hsp70 in earthworms should represent not only a good wide-spectrum biomarker of exposure but also a biomarker of effect since known toxicants altered gene expression in tissues of these animals, as contrasted with a simple accumulation of Hsp. Hence, the detection of Hsp70 in earthworms can constitute an early-warning marker for the presence of potentially deleterious agents in soils, with L. terrestris in particular and earthworms in general acting as potential sentinel animal species.

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Section: Heat Shock Treatment Induces Hsp70mentioning

confidence: 91%

Evaluation for Hsp70 as a biomarker of effect of pollutants on the earthworm Lumbricus terrestris

Nadeau

Corneau²,

Plante³

et al. 2001

Cell Stress Chaper

115

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“…Adult earthworms, from a commercial supplier (Carolina Biological Supply, Burlington, USA), were acclimated in an environmental chamber for 2 weeks prior to experimentation, without light at 10°C, in moistened peat moss supplemented with dry high protein baby cereal (Cooper et al, 1970;Fitzpatrick et al, 1987).…”

Section: Animalsmentioning

confidence: 99%

A non-invasive technique for sequential collection of earthworm (Lumbricus terrestris) leukocytes during subchronic immunotoxicity studies

et al. 1991

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SummaryWe have compared two methods for collecting earthworm leukocytes (coelomocytes) with respect to cell yield, viability and behaviour in immunoassays. Non-invasive extrusion was more efficient than puncturing the coelomic cavity. Extrusion does not produce trauma to earthworms maintained under long term laboratory conditions. Neither technique modified immune assays, as determined by erythrocyte and secretory rosette formation, and phagocytosis, since all 3 were functionally equivalent. After an initial extrusion, sequential leukocyte collections by extrusion are possible at intervals of 6 weeks without affecting total and differential cell counts and rosette formation.

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“…The earthworm, Lumbricus terrestris produces carbonic acid at constant rate when is exposed to temperatures between 10 and 22°C. It can compensate its rates of oxygen consumption between 5 and 15°C after a two week acclimation to these temperatures (Fitzpatrick et al, 1987). The rate of respiration of earthworms is very sensitive to the environmental temperature.…”

Section: Introductionmentioning

confidence: 99%

Temperature-dependent alterations in metabolic enzymes and proteins of three ecophysiologically different species of earthworms

Tripathi¹,

Kachhwaha²,

Dabi³

et al. 2011

Braz. arch. biol. technol.

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Thermal acclimation, preference and effects on .Vo2 in the earthworm Lumbricus terrestris

Cited by 14 publications

References 3 publications

Evaluation for Hsp70 as a biomarker of effect of pollutants on the earthworm Lumbricus terrestris

Evaluation for Hsp70 as a biomarker of effect of pollutants on the earthworm Lumbricus terrestris

A non-invasive technique for sequential collection of earthworm (Lumbricus terrestris) leukocytes during subchronic immunotoxicity studies

Temperature-dependent alterations in metabolic enzymes and proteins of three ecophysiologically different species of earthworms

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