Therapy with resveratrol attenuates obesity-associated allergic airway inflammation in mice

André, Diana Majolli; Calixto, Marina C.; Sollon, Carolina; Alexandre, Eduardo C.; Leiria, Luiz O.; Tobar, Natália; Anhê, Gabriel Forato; Antunes, Edson

doi:10.1016/j.intimp.2016.06.017

Cited by 38 publications

(30 citation statements)

References 41 publications

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“…As AMPK is a target of resveratrol in several model systems181920, we investigated whether resveratrol could modulate the AMPK-mediated pathways that inhibit ICAM-1 expression. Compared with vehicle control-treated cells, significant AMPK activation was observed beginning at 5 min after resveratrol treatment and continuing for 15 min (Fig.…”

Section: Resultsmentioning

confidence: 99%

Resveratrol attenuates ICAM-1 expression and monocyte adhesiveness to TNF-α-treated endothelial cells: evidence for an anti-inflammatory cascade mediated by the miR-221/222/AMPK/p38/NF-κB pathway

Liu

Sung

Lin

et al. 2017

Sci Rep

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Resveratrol, an edible polyphenolic phytoalexin, improves endothelial dysfunction and attenuates inflammation. However, the mechanisms have not been thoroughly elucidated. Therefore, we investigated the molecular basis of the effects of resveratrol on TNF-α-induced ICAM-1 expression in HUVECs. The resveratrol treatment significantly attenuated the TNF-α-induced ICAM-1 expression. The inhibition of p38 phosphorylation mediated the reduction in ICAM-1 expression caused by resveratrol. Resveratrol also decreased TNF-α-induced IκB phosphorylation and the phosphorylation, acetylation, and translocation of NF-κB p65. Moreover, resveratrol induced the AMPK phosphorylation and the SIRT1 expression in TNF-α-treated HUVECs. Furthermore, TNF-α significantly suppressed miR-221/-222 expression, which was reversed by resveratrol. miR-221/-222 overexpression decreased p38/NF-κB and ICAM-1 expression, which resulted in reduced monocyte adhesion to TNF-α-treated ECs. In a mouse model of acute TNF-α-induced inflammation, resveratrol effectively attenuated ICAM-1 expression in the aortic ECs of TNF-α-treated wild-type mice. These beneficial effects of resveratrol were lost in miR-221/222 knockout mice. Our data showed that resveratrol counteracted the TNF-α-mediated reduction in miR-221/222 expression and decreased the TNF-α-induced activation of p38 MAPK and NF-κB, thereby suppressing ICAM-1 expression and monocyte adhesion. Collectively, our results show that resveratrol attenuates endothelial inflammation by reducing ICAM-1 expression and that the protective effect was mediated partly through the miR-221/222/AMPK/p38/NF-κB pathway.

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Section: Resultsmentioning

confidence: 99%

Resveratrol attenuates ICAM-1 expression and monocyte adhesiveness to TNF-α-treated endothelial cells: evidence for an anti-inflammatory cascade mediated by the miR-221/222/AMPK/p38/NF-κB pathway

Liu

Sung

Lin

et al. 2017

Sci Rep

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“…After 4 hours of fasting during the middle of week 15, the mice were injected intraperitoneally with 0.75 U/kg of insulin (Wanbang medicine, Jiangsu, China), and blood glucose levels were measured after 0, 5, 15, 30, and 60 min using an accurate blood glucose meter (Accu-check Performa) [19,20]. The AUC was calculated using the trapezoid rule.…”

Section: Insulin Tolerance Test (Itt)mentioning

confidence: 99%

Protective Effects of 2-Dodecyl-6-Methoxycyclohexa-2,5 -Diene-1,4-Dione Isolated from Averrhoa Carambola L. (Oxalidaceae) Roots on High-Fat Diet-Induced Obesity and Insulin Resistance in Mice

Li¹,

Wei²,

Xie³

et al. 2016

Cell Physiol Biochem

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Background/Aims: The roots of Averrhoa carambola L. (Oxalidaceae) have long been used as a traditional Chinese medicine for the treatment of diabetes and diabetes-related diseases. 2-dodecyl-6-methoxycycyclohexa-2,5-1,4-dione (DMDD) has been isolated from A. carambola L. roots, and this study was carried out to investigate the potential beneficial effects of DMDD on obesity and insulin resistance induced by a high-fat diet (HFD) in mice. Methods: C57BL/6J mice were fed a HFD for 16 weeks and orally administered DMDD (12.5, 25, or 50 mg/kg of body weight per day) and metformin (280 mg/kg of body weight per day) for the last 4 weeks. Results: The body weights and adipose tissue weights as well as the serum levels of blood glucose, total cholesterol, triglycerides, free fatty acids, insulin, interleukin-6, and tumor necrosis factor-α were significantly decreased by DMDD, and the expression of Toll-like receptor 4 (TLR4) and myeloid differentiation factor (Myd88) in the epididymal adipose tissue was downregulated by DMDD. In contrast, insulin sensitivity was enhanced. The results of the glucose tolerance tests, insulin tolerance tests, and insulin release tests indicated that there was a marked improvement in insulin secretion, and the areas under the curve corresponding to the three tests were also significantly decreased by DMDD. The activities of superoxide dismutase and glutathione peroxidase were simultaneously enhanced, whereas the content of malondialdehyde was decreased by DMDD in the liver homogenates of the C57BL/6J mice. In addition, hepatic steatosis and adipocyte hypertrophy, as assessed by H&E staining of liver and adipose tissues, were significantly improved by DMDD. Conclusion: These data suggest that MDD has potential benefits for the treatment of HFD-induced obesity and insulin resistance, and its effects may be associated with improvements in lipid metabolism and inhibition of the expression of TLR4 in adipose tissues.

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“…The mice fed with a high-fat diet (HFD) or a standard chow diet were challenged with OVA to induce asthma. OVA challenge in HFD-fed mice increased eosinophil infiltration in the BAL fluid compared to lean mice, and resveratrol treatment almost abrogated eosinophil infiltration in the obese mice 10. The models reviewed above clearly showed that although resveratrol inhibits the process of inflammation, mechanisms for resveratrol have not yet been elucidated.…”

mentioning

confidence: 99%

“…Also, resveratrol inhibited the expression of the Syk protein and degranulation in mast cells 9. Third, the inhibitory role of resveratrol is evaluated in an obesity-associated allergic pulmonary inflammation 10. The mice fed with a high-fat diet (HFD) or a standard chow diet were challenged with OVA to induce asthma.…”

mentioning

confidence: 99%

“…The clinical use of glucocorticoids has a high risk of side effects, and the effect of glucocorticoids is controversial, especially in noneosinophilic asthma. However, resveratrol has been shown to even suppress the development of noneosinophilic asthma 101415. Therefore, resveratrol and related compounds should be further investigated as alternatives to corticosteroids for the treatment of nonallergic forms of asthma.…”

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confidence: 99%

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Resveratrol in Asthma: A French Paradox?

Kim

2017

Allergy Asthma Immunol Res

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Therapy with resveratrol attenuates obesity-associated allergic airway inflammation in mice

Cited by 38 publications

References 41 publications

Resveratrol attenuates ICAM-1 expression and monocyte adhesiveness to TNF-α-treated endothelial cells: evidence for an anti-inflammatory cascade mediated by the miR-221/222/AMPK/p38/NF-κB pathway

Resveratrol attenuates ICAM-1 expression and monocyte adhesiveness to TNF-α-treated endothelial cells: evidence for an anti-inflammatory cascade mediated by the miR-221/222/AMPK/p38/NF-κB pathway

Protective Effects of 2-Dodecyl-6-Methoxycyclohexa-2,5 -Diene-1,4-Dione Isolated from Averrhoa Carambola L. (Oxalidaceae) Roots on High-Fat Diet-Induced Obesity and Insulin Resistance in Mice

Resveratrol in Asthma: A French Paradox?

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