Therapeutic use of an antibiotic-resistant Bifidobacterium preparation in men exposed to high-dose  -irradiation

Korschunov, V. M.; Smeyanov, V. V.; Ефимов, Б. А.; Tarabrina, N. P.; Иванов, А. А.; Баранов, А Е

doi:10.1099/00222615-44-1-70

Cited by 12 publications

(3 citation statements)

References 14 publications

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“…1987 ). The therapeutic use of an antibiotic‐resistant B. longum by men exposed to high‐dose γ‐irradiation also apparently protected against opportunistic enteric pathogens ( Korschunov et al . 1996 ).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of bifidus milk on the experimental infection withSalmonella enteritidissubsp.typhimuriumin conventional and gnotobiotic mice

Silva¹,

Bambirra²,

Oliveira³

et al. 1999

Journal of Applied Microbiology

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The ability of Bifidobacterium bifidum from a commercial bifidus milk to antagonize Salmonella enteritidis subsp. typhimurium in vivo, and to reduce the pathological consequences for the host, was determined using conventional and gnotobiotic mice. Conventional animals received daily, by gavage, 0·1 ml bifidus milk containing about 109 cfu B. bifidum and germ‐free animals received a single 0·1 ml dose. The conventional and gnotobiotic groups were challenged orally with 102 cfu of the pathogenic bacteria 5 and/or 10 d after the beginning of treatment. Control groups were treated with milk. Bifidus milk protected both animal models against the challenge with the pathogenic bacteria, as demonstrated by survival and histopathological data. However, to obtain the protective effect in gnotobiotic animals, the treatment had to be initiated 10 d before the challenge. In experimental and control gnotobiotic mice, Salm. enteritidis subsp. typhimurium became similarly established at levels ranging from 108 to 109 viable cells g−1 of faeces and remained at these high levels until the animals died or were sacrificed. It was concluded that the protection against Salm. enteritidis subsp. typhimurium observed in conventional and gnotobiotic mice treated with bifidus milk was not due to the reduction of the intestinal populations of the pathogenic bacteria.

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Section: Discussionmentioning

confidence: 99%

Protective effect of bifidus milk on the experimental infection withSalmonella enteritidissubsp.typhimuriumin conventional and gnotobiotic mice

Silva¹,

Bambirra²,

Oliveira³

et al. 1999

Journal of Applied Microbiology

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“…Bifidobacterium is known to be a predominant constituent of the human intestinal microflora (Finegold et al 1977). The bacterium is distributed from the distal part of the small intestine to the large intestine and is believed to have beneficial effects as a probiotic (Kohwi et al 1982;Nakaya 1984;Korschunov et al 1996). Recently, interest has focused on its anti-infectious effects, particularly the age-old concept of using bacterial preparations with inhibitory activity against enteric pathogens for protecting domestic animals and humans.…”

Section: Introductionmentioning

confidence: 99%

“…1995) and is believed to have beneficial effects as a probiotic (Kohwi et al . 1982; Nakaya 1984; Korschunov et al . 1996).…”

mentioning

confidence: 99%

Purification and characterization of a novel protein produced byBifidobacterium longumSBT2928 that inhibits the binding of enterotoxigenicEscherichia coliPb176 (CFA/II) to gangliotetraosylceramide

Fujiwara

Hashiba

Hirota

et al. 1999

Journal of Applied Microbiology

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A novel protein (BIF) which shows inhibitory activity on the binding of enterotoxigenic Escherichia coli Pb176 (ETEC with colonization factor antigen (CFA) II, which consists of coli surface‐associated antigens CS1 and CS3) to gangliotetraosylceramide (asialo GM1 or GA1) was isolated from the culture supernatant fluid of Bifidobacterium longum SBT2928 (BL2928) at its stationary phase. The homogeneity of the final preparation of BIF was demonstrated by SDS‐PAGE, polyacrylamide gel electrofocusing and N‐terminal amino acid sequencing. The BIF was characterized as (i) a protein with an Mr of approximately 104 kDa when chromatographed on a gel filtration column, and 52 kDa when separated on SDS‐PAGE, and (ii) having an isoelectric point of 5·9. No change in size was produced by thiol reduction. These results suggest that BIF is a homodimer consisting of identical 52 kDa monomers. The purified BIF at the concentration of 25 μg protein ml−1 caused a 50% reduction in binding of the ETEC strain to GA1.

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The Family Bifidobacteriaceae

Biavati¹,

Mattarelli²

2006

The Prokaryotes

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Therapeutic use of an antibiotic-resistant Bifidobacterium preparation in men exposed to high-dose -irradiation

Cited by 12 publications

References 14 publications

Protective effect of bifidus milk on the experimental infection withSalmonella enteritidissubsp.typhimuriumin conventional and gnotobiotic mice

Protective effect of bifidus milk on the experimental infection withSalmonella enteritidissubsp.typhimuriumin conventional and gnotobiotic mice

Purification and characterization of a novel protein produced byBifidobacterium longumSBT2928 that inhibits the binding of enterotoxigenicEscherichia coliPb176 (CFA/II) to gangliotetraosylceramide

The Family Bifidobacteriaceae

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