Therapeutic Reactive Oxygen Generation

Scharff, P.; Ritter, Uwe; Matyshevska, Olga; Prylutska, Svitlana; Grynyuk, І. І.; Golub, A. A.; Prylutskyy, Yuriy; Burlaka, Anatoliy

doi:10.1177/030089160809400221

Cited by 33 publications

(29 citation statements)

References 10 publications

(10 reference statements)

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“…C 60 fullerene has an eminent position among the available promising and effective biomedical compounds [1]. Due to their nanoscale dimension, combination of strength with low weight [2], strong antioxidant properties [3,4], accessibility for cellular uptake [5][6][7], the pristine C 60 fullerenes are considered as pharmaceutically valuable compounds of a new class [8][9][10].…”

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confidence: 99%

Toxic effect of C60 fullerene-doxorubicin complex towards tumor and normal cells in vitro

et al. 2014

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Creation of new nanostructures possessing high antitumor activity is an important problem of modern biotechnology. Aim. To evaluate cytotoxicity of created complex of pristine C 60 fullerene with the anthracycline antibiotic doxorubicin (Dox), as well as of free C 60 fullerene and Dox, towards different cell types-tumor, normal immunocompetent and hepatocytes. Methods. Measurement of size distribution for particles in C 60 + Dox mixture was performed by a dynamic light scattering (DLS) technique. Toxic effect of C 60 + Dox complex in vitro towards tumor and normal cells was studied using the MTT assay. Results. DLS experiment demonstrated that the main fraction of the particles in C 60 + Dox mixture had a diameter in the range of about 132 nm. The toxic effect of C 60 + Dox complex towards normal (lymphocytes, macrophages, hepatocytes) and tumor (Ehrlich ascites carcinoma, leukemia L1210, Lewis lung carcinoma) cells was decreased by~10-16 % and~7-9 %, accordingly, compared with the same effect of free Dox. Conclusions. The created C 60 + Dox composite may be considered as a new pharmacological agent that kills effectively tumor cells in vitro and simultaneously prevents a toxic effect of the free form of Dox on normal cells.

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confidence: 99%

Toxic effect of C60 fullerene-doxorubicin complex towards tumor and normal cells in vitro

et al. 2014

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“…Since we did not find significant decrease in L1210 cell viability after 24 h treatment with 1 μg/ml of CP (Fig. 1), as well as after fullerene C 60 loading [22], we can assume that a decrease of mitochondrial potential in L1210 cells treated with these effectors may be of adaptive nature and does not affect cells' survival after longer period of incubation.…”

Section: Fig 2 Relative Value Of Mitochondrial Membrane Potential Imentioning

confidence: 66%

Modulation of cisplatin-induced reactive oxygen species production by fullerene C(60) in normal and transformed lymphoid cells

Franskevych¹,

Grynyuk²,

Prylutska³

et al. 2016

Ukr.Biochem.J.

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The early response of normal (Wistar rat thymocytes) and transformed (mice lymphoid leukemia L1210) cells to treatment with anticancer drug cisplatin or to combined treatment with cisplatin and carbon nanostructure fullerene C60 was studied. We demonstrated with fluorescent probes DCFH-DA and TMRE that cisplatin at concentration 1 μg/ml induced reactive oxygen species (ROS) production and decreased the value of mitochondrial membrane potential in both cell types. The combined treatment with cisplatin (1 μg/ml) and fullerene C60 (7.2 μg/ml) was shown to be followed by oppositely directed modulation of ROS production in thymocytes and L1210 cells. Cisplatin-induced ROS production was intensified in L1210 cells, while in thymocytes it was decreased. It is supposed that the different effects of combined treatment are associated with peculiarities of fullerene C60 accumulation and localization in normal and cancer cells.

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“…Despite the common notion that photodamaging effect of C 60 on cancer cells is determined by effective production of cytotoxic ROS in intracellular space [31, 32], the biochemical pathways and signaling networks of its realization still need to be elucidated.…”

Section: Resultsmentioning

confidence: 99%

Fullerene C60 Penetration into Leukemic Cells and Its Photoinduced Cytotoxic Effects

et al. 2017

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Fullerene C60 as a representative of carbon nanocompounds is suggested to be promising agent for application in photodynamic therapy due to its unique physicochemical properties. The goal of this study was to estimate the accumulation of fullerene C60 in leukemic cells and to investigate its phototoxic effect on parental and resistant to cisplatin leukemic cells. Stable homogeneous water colloid solution of pristine C60 with average 50-nm diameter of nanoparticles was used in experiments. Fluorescent labeled C60 was synthesized by covalent conjugation of C60 with rhodamine B isothiocyanate. The results of confocal microscopy showed that leukemic Jurkat cells could effectively uptake fullerene C60 from the medium. Light-emitting diode lamp (100 mW cm−2, λ = 420–700 nm) was used for excitation of accumulated C60. A time-dependent decrease of viability was detected when leukemic Jurkat cells were exposed to combined treatment with C60 and visible light. The cytotoxic effect of photoexcited C60 was comparable with that induced by H2O2, as both agents caused 50% decrease of cell viability at 24 h at concentrations about 50 μM. Using immunoblot analysis, protein phosphotyrosine levels in cells were estimated. Combined action of C60 and visible light was followed by decrease of cellular proteins phosphorylation on tyrosine residues though less intensive as compared with that induced by H2O2 or protein tyrosine kinase inhibitor staurosporine. All tested agents reduced phosphorylation of 55, 70, and 90 kDa proteins while total suppression of 26 kDa protein phosphorylation was specific only for photoexcited C60.The cytotoxic effect of C60 in combination with visible light irradiation was demonstrated also on leukemic L1210 cells both sensitive and resistant to cisplatin. It was shown that relative value of mitochondrial membrane potential measured with tetramethylrhodamine ethyl ester perchlorate (TMRE) probe was lower in resistant cells in comparison with sensitive cells and the drop of mitochondrial potential corresponded to further decrease of resistant cell viability after C60 photoexcitation. The data obtained allow to suggest that C60-mediated photodynamic treatment is a candidate for restoration of drug-resistant leukemic cell sensitivity to induction of mitochondrial way of apoptosis.

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Therapeutic Reactive Oxygen Generation

Cited by 33 publications

References 10 publications

Toxic effect of C60 fullerene-doxorubicin complex towards tumor and normal cells in vitro

Toxic effect of C60 fullerene-doxorubicin complex towards tumor and normal cells in vitro

Modulation of cisplatin-induced reactive oxygen species production by fullerene C(60) in normal and transformed lymphoid cells

Fullerene C60 Penetration into Leukemic Cells and Its Photoinduced Cytotoxic Effects

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