Therapeutic prospects of hydroxytyrosol on experimentally induced diabetic testicular damage: potential interplay with AMPK expression

Alsemeh, Amira Ebrahim; Samak, Mai A.; El-Fatah, Samaa Salah Abd

doi:10.1007/s00441-019-03143-2

Cited by 12 publications

(6 citation statements)

References 54 publications

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“…Our biochemical results supported the previous theory that the HT has antioxidant properties as there was significant increase in mean brain antioxidant biomarkers levels (GPx, and SOD) and significant decrease in MDA level of CYP and HT-treated in comparison to CYP-treated group. These finding are in consistence with Ebrahimi & Moghaddam [57] and Soni, et al [58] .…”

Section: Discussionsupporting

confidence: 89%

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Alsemeh

Abdel-Rahman

Mahmoud

et al. 2020

Journal of Medical Histology

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Cypermethrin (CYP) is a synthetic pyrethroid. It crosses the blood-brain barrier in pests and creates neurotoxicity in the CNS. Hydroxytyrosol (HT) is considered a powerful natural antioxidant, it is found in olive leaf, olive pulp and olive oil. Hydroxytyrosol is the only phenol that is capable to pass the blood-brain barrier and permits it to involve free radicals throughout the CNS. We designed this study to explore the possible protective role of HT against the neurotoxic effect induced by CYP. Thirty male adult albino rats were divided into three groups. Control group; subdivided into: control, corn oil, HT groups, CYP treated and CYP, CYP+ HT treated group. Treatments were given by gavage for 14 days. The cerebrum tissue was prepared for histopathological examination and immunohistochemical detection of caspase 3 and GFAP. We also assessed the level of GAPA and ACH in the tissue and the oxidative enzymatic and the lipid perioxidation profile. We observed that the concomitant use of HT with the CYP diminished the signs of hyper excitability induced by the CYP which confirmed by significant decrease in the level of GABA in cerebrum tissue. HT counteracted the histopathological changes induced by CYP and significantly downregulated the caspase -3 and GFAP expression in cerebrum tissue. A rescue of the oxidative enzymatic activity, lipid peroxidation profiles was also evident in the CYP+HT-treated group. We concluded that HT exhibited substantial antioxidant and anti-apoptotic impacts on the cerebral cortex cytomorphological alterations and apoptosis produced by CYP in cerebrum tissue.

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Section: Discussionsupporting

confidence: 89%

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Alsemeh

Abdel-Rahman

Mahmoud

et al. 2020

Journal of Medical Histology

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“…According to the literature, the effect of HT on these pathways is controversial. Some authors have pointed that HT upregulates AMPK 56 and inhibits Akt phosphorylation in tumor and non-tumor cells at 50, 100 and 200 µM [57][58][59] . However, HT has also been shown to promote Akt phosphorylation 60 .…”

Section: Discussionmentioning

confidence: 99%

Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells

Calahorra

Martı́nez-Lara

Granadino‐Roldán

et al. 2020

Sci Rep

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Olive oil intake has been linked with a lower incidence of breast cancer. Hypoxic microenvironment in solid tumors, such as breast cancer, is known to play a crucial role in cancer progression and in the failure of anticancer treatments. HIF-1 is the foremost effector in hypoxic response, and given that hydroxytyrosol (HT) is one of the main bioactive compounds in olive oil, in this study we deepen into its modulatory role on HIF-1. Our results in MCF-7 breast cancer cells demonstrate that HT decreases HIF-1α protein, probably by downregulating oxidative stress and by inhibiting the PI3K/ Akt/mTOR pathway. Strikingly, the expression of HIF-1 target genes does not show a parallel decrease. Particularly, adrenomedullin and vascular endothelial growth factor are up-regulated by high concentrations of HT even in HIF-1α silenced cells, pointing to HIF-1-independent mechanisms of regulation. In fact, we show, by in silico modelling and transcriptional analysis, that high doses of HT may act as an agonist of the aryl hydrocarbon receptor favoring the induction of these angiogenic genes. In conclusion, we suggest that the effect of HT in a hypoxic environment is largely affected by its concentration and involves both HIF-1 dependent and independent mechanisms.

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“…Image analysis: The photos were taken using LEICA DM500 optical microscope at Zagazig University's department of anatomy and embryology. Quantitative measurements were made using Image J analysis software (Fiji Image J; 1.51 n, NIH, USA) to determine the mean area percentage of collagen fibers, the percentage of α-SMA-positive cells, the number of immunoreactive cells for caspase-3 in various stained sections, the number of Leydig cells/100 µm2, the number of Sertoli cells/tubule, and the thickness of peritubular myoid cells (µm) in 6 animals/group ,150 seminiferous tubules at 400× mag [32].…”

Section: Tissue Preparation For Histopathological and Immunohistochem...mentioning

confidence: 99%

Protective Effect of Resveratrol against Testicular Dysfunction Induced by Forced Swimming Exercise in Adult Rats

Khalil

Abdelghany

Fatah

et al. 2022

Zagazig University Medical Journal

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Background:The effect of vigorous exercise on testicular function is poorly understood. This work aims to identify the resveratrol influence on the altered reproductive function induced by forced exercise and to screen the relevant possible mechanisms. Methods: The study comprised control, forced exercise (FE), and resveratrol treatment groups carried out on a total of 24 adult male rats. After 8 weeks of the exercise, the pituitary and testicular hormones, corticosterone, tumor necrosis factor (TNF-α), IL-6, epididymal sperm parameters, testicular oxidantantioxidant markers, testicular ornithine decarboxylase (ODC) activity, polyamines, testicular histopathology, and immunohistochemistry for apoptotic reactions were all measured. Results: The FE group showed significant reductions in the pituitary and testicular hormones, sperm count, and motility, while corticosterone, TNFα, IL-6 serum levels, and aberrant forms of sperms were significantly increased. Testicular tissue displayed abnormal architecture, apoptotic changes with a significantly increased malondialdehyde level, ODC activity and polyamines, and a reduction in superoxide dismutase activity. In comparison to the FE group, administration of resveratrol significantly improved the gonadal hormones, corticosterone, TNF-α, IL-6 levels, sperm parameters, oxidant-antioxidant system, ODC activity, and polyamines with an enhancement of testicular architecture and apoptosis. Conclusion: Resveratrol was effective in attenuating testicular dysfunction caused by forced swimming exercise in rats.

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Therapeutic prospects of hydroxytyrosol on experimentally induced diabetic testicular damage: potential interplay with AMPK expression

Cited by 12 publications

References 54 publications

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells

Protective Effect of Resveratrol against Testicular Dysfunction Induced by Forced Swimming Exercise in Adult Rats

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