Therapeutic potential of sodium selenite in letrozole induced polycystic ovary syndrome rat model: Targeting mitochondrial approach (selenium in PCOS)

Atef, Marwa Mohamed; Abdellatif, Rania; Emam, Marwa Nagy; Gheit, Rehab E. Abo El; Amer, Alaa; Hafez, Yasser Mostafa

doi:10.1016/j.abb.2019.06.009

Cited by 38 publications

(36 citation statements)

References 66 publications

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“…Firstly, the letrozole-induced PCOS model used in this study has been widely utilized to assess the effects and the mechanisms of interventions on precautions and pathogenesis in PCOS ( 46 – 48 ). Letrozole, an aromatase inhibitor, induces hyperandrogenemia by reducing the conversion of T to estrogen ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

Dietary α-Linolenic Acid-Rich Flaxseed Oil Exerts Beneficial Effects on Polycystic Ovary Syndrome Through Sex Steroid Hormones—Microbiota—Inflammation Axis in Rats

Wang

Sha

et al. 2020

Front. Endocrinol.

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Polycystic ovary syndrome (PCOS) represents a common endocrine-metabolic disorder disease with chronic low-grade inflammation and alteration of intestinal flora. Serving as functional food, flaxseed oil (FO), which is rich in plant-derived α-linolenic acid of omega-3 polyunsaturated fatty acids, has been proven to benefit for chronic metabolic diseases. However, the exact role of dietary FO on PCOS remains largely unclear. In the present study, 6-week-old female Sprague-Dawley rats were randomly divided into four groups (eight rats/group), including (a) pair-fed (PF) control (CON) group (PF/CON), (b) FO-fed CON group (FO/CON), (c) PF with letrozole-induced PCOS model (MOD) group (PF/MOD), and (d) FO-fed MOD group (FO/MOD). All rats were fed a standard diet. After 3 weeks of modeling and subsequent 8 weeks of treatment, the rats in diverse groups were euthanized and associated indications were investigated. The results showed that dietary FO ameliorated the disorder of estrous cycle and ovarian morphology. In parallel, dietary FO improved the sex steroid hormone disturbance (luteinizing hormone/follicle-stimulating hormone, estrogen, testosterone, and progesterone), body weights, dyslipidemia, and insulin resistance. Moreover, FO treatment improved plasma and ovary inflammatory interleukin (IL)-1β, IL-6, IL-10, and IL-17A, tumor necrosis factor-α, and monocyte chemoattractant protein-1. Additionally, FO intervention significantly modulated the composition of gut microbiota and vaginal microbiota by increasing the abundances of Allobaculum, Lactobacillus, Butyrivibrio, Desulfovibrio, Bifidobacterium, Faecalibacterium, Parabacteroides as well as decreasing the abundances of Actinobacteria, Bacteroides, Proteobacteria, and Streptococcus, the ratio of Firmicutes/Bacteroidetes. A decrease in plasma lipopolysaccharide level and an increase in short-chain fatty acids, including acetic acid, propionic acid, butyric acid and pentanoic acid, were determined after dietary FO supplementation. Correlation analysis Wang et al. FO Ameliorates PCOS in Rats revealed close relationships among sex steroid hormones, inflammation, and gut/vaginal microbiota. Collectively, this study demonstrated that dietary FO ameliorated PCOS through the sex steroid hormones-microbiota-inflammation axis in rats, which may contribute to the understanding of pathogenesis and potentially serve as an inexpensive intervention in the control of PCOS.

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Section: Discussionmentioning

confidence: 99%

Dietary α-Linolenic Acid-Rich Flaxseed Oil Exerts Beneficial Effects on Polycystic Ovary Syndrome Through Sex Steroid Hormones—Microbiota—Inflammation Axis in Rats

Wang

Sha

et al. 2020

Front. Endocrinol.

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“…It was shown that Se was implicated in regulating the physiology of uterine smooth muscles potentially via modulating the expression of Selenon, Selenot, and Selenow [41,42], and the G protein Rho (RhoA)/Rho kinase (ROCK) signaling pathway in mice [43]. However, only recently have researchers paid attention to potential role of Se in ovarian physiology and embryo development [9,28,[44][45][46][47][48][49][50]. Interestingly, when exploring the mainstream literature, with the exclusion of one study reporting the beneficial effects of Se supplementation on sperm parameters in aging mice [51], no study has yet elucidated how Se might ameliorate the reproductive function in aging mammalian models, let alone the aged females.…”

Section: Introductionmentioning

confidence: 99%

Dietary Selenium Supplementation Ameliorates Female Reproductive Efficiency in Aging Mice

et al. 2019

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Female reproductive (ovarian) aging is distinctively characterized by a markedly reduced reproductive function due to a remarkable decline in quality and quantity of follicles and oocytes. Selenium (Se) has been implicated in playing many important biological roles in male fertility and reproduction; however, its potential roles in female reproduction, particularly in aging subjects, remain poorly elucidated. Therefore, in the current study we used a murine model of female reproductive aging and elucidated how different Se-levels might affect the reproductive efficiency in aging females. Our results showed that at the end of an 8-week dietary trial, whole-blood Se concentration and blood total antioxidant capacity (TAOC) were significantly reduced in Se-deficient (0.08 mg Se/kg; Se-D) mice, whereas both of these biomarkers were significantly higher in inorganic (0.33 mg/kg; ISe-S) and organic (0.33 mg/kg; OSe-S) Se-supplemented groups. Similarly, compared to the Se-D group, Se supplementation significantly ameliorated the maintenance of follicles and reduced the rate of apoptosis in ovaries. Meanwhile, the rate of in vitro-produced embryos resulting from germinal vesicle (GV) oocytes was also significantly improved in Se-supplemented (ISe-S and OSe-S) groups compared to the Se-D mice, in which none of the embryos developed to the hatched blastocyst stage. RT-qPCR results revealed that mRNA expression of Gpx1, Gpx3, Gpx4, Selenof, p21, and Bcl-2 genes in ovaries of aging mice was differentially modulated by dietary Se levels. A considerably higher mRNA expression of Gpx1, Gpx3, Gpx4, and Selenof was observed in Se-supplemented groups compared to the Se-D group. Similarly, mRNA expression of Bcl-2 and p21 was significantly lower in Se-supplemented groups. Immunohistochemical assay also revealed a significantly higher expression of GPX4 in Se-supplemented mice. Our results reasonably indicate that Se deficiency (or marginal levels) can negatively impact the fertility and reproduction in females, particularly those of an advancing age, and that the Se supplementation (inorganic and organic) can substantiate ovarian function and overall reproductive efficiency in aging females.

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“…It has been observed to possess a cardioprotective property, along with diminishing before-and after-meal glucose levels, assisting in reduction of weight and diminishing lipid levels in plasma. 7,8 A preclinical study of cardiac failure and myocardial infarction (MI) has demonstrated that metformin expands the resilience of the heart to ischemia-reperfusion damage and, after infarction, diminishes the advancement of heart failure. 9,10 The UK Prospective Diabetes Study (UKPDS) showed that metformin beneficially affects diverse cardiovascular hazard factors.…”

Section: Introductionmentioning

confidence: 99%

“…Metformin is a medication utilized in patients with Type 2 diabetes (T2D). It has been observed to possess a cardioprotective property, along with diminishing before‐ and after‐meal glucose levels, assisting in reduction of weight and diminishing lipid levels in plasma . A preclinical study of cardiac failure and myocardial infarction (MI) has demonstrated that metformin expands the resilience of the heart to ischemia–reperfusion damage and, after infarction, diminishes the advancement of heart failure .…”

Section: Introductionmentioning

confidence: 99%

Cardioprotective role of metformin against sodium arsenite‐induced oxidative stress, inflammation, and apoptosis

et al. 2019

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Arsenic is a universal component and a notable natural poison. Its exposure to people occurs primarily through natural, therapeutic, and occupational sources. This investigation intended to discover the defensive impact of metformin (100 and 150 mg/kg body weight) against sodium arsenite (SA)‐induced cardiotoxicity in experimental animals. The study was conducted on Sprague Dawley rats (n = 50), which were separated into five different groups as follows: control, met‐150, SA, SA + met‐100, and SA + met‐150. The results demonstrated that SA caused a significant increase in the level of malondialdehyde and also reduced activities of antioxidative enzyme. SA similarly increased inflammatory reactions by increasing the level of interleukin‐6, tumor necrosis factor‐α, and interleukin‐1β. In addition, SA provoked the apoptosis by expanding the p53 and Bax levels, terminal deoxynucleotidyl transferase dUTP nick end labeling, and expression of caspase‐3. SA altered the histological integrity of cardiac tissue and 8‐hydroxy‐2′‐deoxyguanosine expression. In conclusion, metformin significantly reduced oxidative stress, inflammatory reaction, and apoptotic pathway. The present investigation showed that metformin has a cardioprotective impact because of its protective role against oxidation, inflammation, and apoptosis.

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Therapeutic potential of sodium selenite in letrozole induced polycystic ovary syndrome rat model: Targeting mitochondrial approach (selenium in PCOS)

Cited by 38 publications

References 66 publications

Dietary α-Linolenic Acid-Rich Flaxseed Oil Exerts Beneficial Effects on Polycystic Ovary Syndrome Through Sex Steroid Hormones—Microbiota—Inflammation Axis in Rats

Dietary α-Linolenic Acid-Rich Flaxseed Oil Exerts Beneficial Effects on Polycystic Ovary Syndrome Through Sex Steroid Hormones—Microbiota—Inflammation Axis in Rats

Dietary Selenium Supplementation Ameliorates Female Reproductive Efficiency in Aging Mice

Cardioprotective role of metformin against sodium arsenite‐induced oxidative stress, inflammation, and apoptosis

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