Therapeutic Potential of Human Adipose-Derived Stem Cell Exosomes in Stress Urinary Incontinence – An in Vitro and in Vivo Study

Ni, Jianshu; Li, Hongchao; Zhou, Yiwen; Gu, Baojun; Xu, Yuhong; Fu, Qiang; Peng, Xufeng; Cao, Nailong; Fu, Qingchun; Jin, Meng; Sun, Guangxi; Wang, Jihong; Jin, Yinpeng; Liu, Feng

doi:10.1159/000492298

Cited by 51 publications

(45 citation statements)

References 41 publications

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“…Accumulating evidence indicates that exosomes derived from ADSCs have the ability to treat various diseases, including diabetes ( Zhao et al, 2018 ), stress urinary incontinence ( Ni et al, 2018 ), amyotrophic lateral sclerosis ( Lee et al, 2016 ), and ischemia/reperfusion injuries ( Pu et al, 2017 ). To examine their effect on a model of ischemic injury, we isolated primary ADSCs from C57/Bl6 mice.…”

Section: Resultsmentioning

confidence: 99%

Exosomes Derived From CircAkap7-Modified Adipose-Derived Mesenchymal Stem Cells Protect Against Cerebral Ischemic Injury

Jiang

et al. 2020

Front. Cell Dev. Biol.

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Background Cerebral ischemic injury is a complicated pathological process. Adipose-derived stromal cells (ADSCs) have been used as a therapeutic strategy, with their therapeutic effects chiefly attributed to paracrine action rather than trans- differentiation. Studies have shown that circAkap7 was found to be downregulated in a mouse model of transient middle cerebral artery occlusion (tMCAO). Methods To explore whether exosomes derived from circAkap7-modified ADSCs (exo-circAkap7) have therapeutic effects on cerebral ischemic injury, a mouse model of tMCAO, as well as an in vitro model of oxygen and glucose deprivation-reoxygenation (OGD-R) in primary astrocytes, were used. Results Results showed that treatment with exo-circAkap7 protected against tMCAO in mice, and in vitro experiments confirmed that co-culture with exo-circAkap7 attenuated OGD-R-induced cellular injury by absorbing miR-155-5p, promoting ATG12-mediated autophagy, and inhibiting NRF2-mediated oxidative stress. Conclusion We demonstrate here that exo-circAkap7 protected against cerebral ischemic injury by promoting autophagy and ameliorating oxidative stress.

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Section: Resultsmentioning

confidence: 99%

Exosomes Derived From CircAkap7-Modified Adipose-Derived Mesenchymal Stem Cells Protect Against Cerebral Ischemic Injury

Jiang

et al. 2020

Front. Cell Dev. Biol.

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“…It has been previously reported that exosomes derived from stromal fibroblasts could enhance the drug resistance to colorectal cancer [37]. Furthermore, adipose-derived stem cells could secrete exosomes, which in turn exhibit biological effects on tissue regeneration and could protect against tissues of bone, muscle, skin, and brain [38].…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of THY1 attenuates interstitial pulmonary fibrosis and promotes lung fibroblast apoptosis during acute interstitial pneumonia by blockade of the WNT signaling pathway

et al. 2019

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Acute interstitial pneumonia (AIP) is an idiopathic pulmonary disease featuring rapid progressive dyspnea and respiratory failure. These symptoms typically develop within several days or weeks in patients without any pre-existing lung disease or external chest disease. Thymocyte differentiation antigen-1 (THY1) has been reported to have an effect on lung fibroblast proliferation and fibrogenic signaling. In this study, the mechanism of THY1 in AIP in influencing pulmonary fibrosis in terms of lung fibroblast proliferation and apoptosis was examined. An AIP mouse model with the pathological changes of lung tissues observed was established to identify the role of THY1 in the pathogenesis of AIP. The expression of THY1, a key regulator of the WNT pathway β-catenin and fibroblasts markers MMP-2, Occludin, α-SMA and Vimentin were determined. Lung fibroblasts of mice were isolated, in which THY1 expression was altered to identify roles THY1 plays in cell viability and apoptosis. A TOP/TOPflash assay was utilized to determine the activation of WNT pathway. Decrement of pulmonary fibrosis was achieved through THY1 up-regulation. The expression of MMP-2, Occludin, α-SMA, Vimentin and β-catenin, and the extent of β-catenin phosphorylation, significantly decreased, thereby indicating that THY1 overexpression inactivated WNT. Cell proliferation was inhibited and apoptosis was accelerated in lung fibroblasts transfected with vector carrying overexpressed THY1. Altogether, this study defines the potential role of THY1 in remission of AIP, via the upregulation of THY1, which renders the WNT pathway inactive. This inactivation of the WNT signaling pathway could alleviate pulmonary fibrosis by reducing lung fibroblast proliferation in AIP.

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“…This new MSCs-based therapy, known as acellular therapy, can provide therapeutic benefits without the need to transplant living cells, making the process easier to be standardized and reducing cell transplant related risks. Several attempts have been made to investigate the therapeutic effects of MSC secretome in PFDs (Table 3), by utilizing the concentrated conditioned media (CCM) of MSCs [64,88] or the exosomes derived from MSCs [89] [90].…”

Section: Mscs-derived Secretome For Pfdsmentioning

confidence: 99%

MSC-based therapy in female pelvic floor disorders

Sima

Chen

2020

Cell Biosci

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Mesenchymal stem cells (MSCs), also referred to as multipotent stromal cells or mesenchymal stromal cells, are present in multiple tissues and capable of differentiating into diverse cell lineages, holding a great promise in developing cell-based therapy for a wide range of conditions. Pelvic floor disorders (PFDs) is a common degenerative disease in women and may diminish a woman’s quality of life at any age. Since the treatments for this disease are limited by the high rates of recurrence and surgical complications, seeking an ideal therapy in the restoration of pelvic floor function is an urgent issue at present. Herein, we summarize the cell sources of MSCs used for PFDs and discuss the potential mechanisms of MSCs in treating PFDs. Specifically, we also provide a comprehensive review of current preclinical and clinical trials dedicated to investigating MSC-based therapy for PFDs. The novel therapy has presented promising therapeutic effects which include relieving the symptoms of urinary or fecal incontinence, improving the biological properties of implanted meshes and promoting the injured tissue repair. Nevertheless, MSC-based therapies for PFDs are still experimental and the unstated issues on their safety and efficacy should be carefully addressed before their clinical applications.

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Therapeutic Potential of Human Adipose-Derived Stem Cell Exosomes in Stress Urinary Incontinence – An in Vitro and in Vivo Study

Cited by 51 publications

References 41 publications

Exosomes Derived From CircAkap7-Modified Adipose-Derived Mesenchymal Stem Cells Protect Against Cerebral Ischemic Injury

Exosomes Derived From CircAkap7-Modified Adipose-Derived Mesenchymal Stem Cells Protect Against Cerebral Ischemic Injury

Up-regulation of THY1 attenuates interstitial pulmonary fibrosis and promotes lung fibroblast apoptosis during acute interstitial pneumonia by blockade of the WNT signaling pathway

MSC-based therapy in female pelvic floor disorders

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