Therapeutic Potential of Antimicrobial Peptides for Wound Healing

Nasseri, Sepideh; Sharifi, Mohammadreza

doi:10.1007/s10989-021-10350-5

Cited by 11 publications

(2 citation statements)

References 94 publications

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“…Both EVs and proteins can be found in elevated levels at sites of infection, where mammalian EVs act as transporting agents delivering vital biomolecules for wound healing (Narauskaitė et al., 2021), whereas outer membrane vesicles (OMVs) in the hostile bacterial colonies are responsible for cell‐cell communication and delivering components required to mitigate host immune answers (Sartorio et al., 2021). In this respect numerous host peptides are overexpressed at these infectious sites, including LL‐37 (Nasseri & Sharifi, 2022). The natural peptide interaction with mammalian EVs at these locations could aid cargo redistribution, whereas more membrane disrupting AMPs could disrupt EVs and release cytosolic components that are relevant for wound healing.…”

Section: Discussionmentioning

confidence: 99%

Removal and identification of external protein corona members from RBC‐derived extracellular vesicles by surface manipulating antimicrobial peptides

Singh

Szigyártó

Ricci

et al. 2023

J of Extracellular Bio

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In the last years, extracellular vesicles (EVs), secreted by various cells and body fluids have shown extreme potential in biomedical applications. Increasing number of studies suggest that a protein corona could adhere to the surface of EVs which can have a fundamental effect on their function, targeting and therapeutical efficacy. However, removing and identifying these corona members is currently a challenging task to achieve. In this study we have employed red blood cell‐derived extracellular vesicles (REVs) as a model system and three membrane active antimicrobial peptides (AMPs), LL‐37, FK‐16 and CM15, to test whether they can be used to remove protein corona members from the surface of vesicles. These AMPs were reported to preferentially exert their membrane‐related activity via one of the common helical surface‐covering models and do not significantly affect the interior of lipid bilayer bodies. The interaction between the peptides and the REVs was followed by biophysical techniques, such as flow‐linear dichroism spectroscopy which provided the effective applicable peptide concentration for protein removal. REV samples were then subjected to subsequent size exclusion chromatography and to proteomics analysis. Based on the comparison of control REVs with the peptide treated samples, seventeen proteins were identified as external protein corona members. From the three investigated AMPs, FK‐16 can be considered as the best candidate to further optimize EV‐related applicability of AMPs. Our results on the REV model system envisage that membrane active peptides may become a useful set of tools in engineering and modifying surfaces of EVs and other lipid‐based natural particles.

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Section: Discussionmentioning

confidence: 99%

Removal and identification of external protein corona members from RBC‐derived extracellular vesicles by surface manipulating antimicrobial peptides

Singh

Szigyártó

Ricci

et al. 2023

J of Extracellular Bio

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“…[76][77][78][79][80] Nevertheless, presence of antibiotic resistant bacteria in the wound site results in delay of the wound healing and must be well considered while the development of wound healing methodologies. 81 AMPs present favorable wound repairing properties with playing a role in all steps of wound healing and possessing an antimicrobial activity. 82 For instance, Takahashi et al demonstrated that Human β-Defensin-3 AMP accelerates the wound healing by triggering the migration and proliferation of human fibroblast with expression of angiogenic growth factors.…”

Section: Discussionmentioning

confidence: 99%

Effect of antimicrobial peptide conjugated surgical sutures on multiple drug-resistant microorganisms

et al. 2022

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Surgical site infections are commonly encountered as a risk factor in clinics that increase the morbidity of a patient after a surgical operation. Surgical sutures are one of the leading factor for the formation of surgical site infections that induce bacterial colonization by their broad surface area. Current strategies to overcome with surgical site infections consist utilization of antibiotic agent coatings such as triclosan. However, the significant increase in antibiotic resistance majorly decreases their efficiency against recalcitrant pathogens such as; Pseudomonas aeruginosa and Staphylococcus aureus. Therefore, the development of a multi drug-resistant antimicrobial suture without any cytotoxic effect to combat surgical site infections is vital. Antimicrobial peptides are the first defense line which has a broad range of spectrum against Gram-positive, and Gram-negative bacteria and even viruses. In addition, antimicrobial peptides have a rapid killing mechanism which is enhanced by membrane disruption and inhibition of functional proteins in pathogens without the development of antimicrobial resistance. In the scope of the current study, the antimicrobial effect of antimicrobial peptide conjugated poly (glycolic acid-co-caprolactone) (PGCL) sutures were investigated against P. aeruginosa and methicillin-resistant S. aureus (MRSA) strains by using antimicrobial peptide sequences of KRFRIRVRV-NH2, RWRWRWRW-NH2 and their dual combination (1:1). In addition, in vitro wound scratch assays were performed to evaluate the effect of antimicrobial peptide conjugated sutures on keratinocyte cell lines. Our results indicated that antimicrobial peptide modified sutures could be a potential novel medical device to overcome surgical site infections by the superior acceleration of wound healing.

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