Therapeutic Potential of a Scorpion Venom-Derived Antimicrobial Peptide and Its Homologs Against Antibiotic-Resistant Gram-Positive Bacteria

Liu, Gaomin; Yang, Fan; Li, Fangfang; Li, Zhongjie; Lang, Yange; Shen, Bingzheng; Wu, Yingliang; Li, Wenxin; Harrison, Patrick L.; Strong, Peter; Xie, Yingqiu; Miller, Keith; Cao, Zhijian

doi:10.3389/fmicb.2018.01159

Cited by 40 publications

(24 citation statements)

References 46 publications

(53 reference statements)

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“…There is also emerging evidence of their efficacy in vivo. One recent example demonstrated the recovery of mice from a lethal bacteraemia caused by clinical methicillin-resistant Staphylococcus aureus through the administration of an AMP derived from scorpion venom [67]. However, challenges also remain with respect to the biostability and bioavailability of these AMPs such that some form of physicochemical optimisation (i.e., PEGylation, peptide cyclisation) are often required, particularly when considering using routes of administration other than parenteral for peptides [68,69].…”

Section: Pain Therapeutics and Congenital Neurological Conditionsmentioning

confidence: 99%

Friends or Foes? Emerging Impacts of Biological Toxins

Clark

Casewell

Elliott

et al. 2019

Trends in Biochemical Sciences

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Toxins are substances produced from biological sources (e.g., animal, plants, microorganisms) that have deleterious effects on a living organism. Despite the obvious health concerns of being exposed to toxins, they are having substantial positive impacts in a number of industrial sectors. Several toxin-derived products are approved for clinical, veterinary, or agrochemical uses. This review sets out the case for toxins as 'friends' that are providing the basis of novel medicines, insecticides, and even nucleic acid sequencing technologies. We also discuss emerging toxins ('foes') that are becoming increasingly prevalent in a range of contexts through climate change and the globalisation of food supply chains and that ultimately pose a risk to health. Toxins and Toxinology In the natural world, toxins are employed for diverse purposes, from the prey-incapacitating molecules found in snake, spider, and cone snail venom, to the defensive compounds harboured by numerous poisonous plant and animal species. While the consequences of human intoxication can be severe (Box 1), the functional diversity of biological toxins has led to their frequent use as experimental tools for studying physiological and pharmacological mechanisms (e.g., synaptic transmission, ion channel subtypes) (Figure 1). Toxinology involves the identification, characterisation, production, and engineering of biological toxins along with their application or repurposing as research tools and clinical products. As such, toxinology encompasses the study of the evolution, chemistry, biology, and clinical effects of toxins and includes potential biotechnological and/or therapeutic applications. Experimental applications of toxins as tools for physiologists go back a long time, including Claude Bernard's experiments in the 1800s with curare to demonstrate the existence of chemical signalling between nerves and muscles [2], and Henry Dale's use of muscarine and nicotine to show different subtypes of receptors for acetylcholine [3]. Snake toxins were critical for the first isolation of a receptor for a neurotransmitter [a-bungarotoxin and nicotinic acetylcholine receptors (nAChRs)] [4]. Recently, peptide toxins have been very useful in teasing out the functional importance of different subtypes of ion channels that are critical for neuronal function and cellular signalling [5-7]. An increasing number of toxins are now transitioning from the laboratory to the clinic and the balance of research in this aspect of toxinology is shifting from the classical development of anti-toxins and anti-venoms towards drug discovery [8,9]. The attraction of toxins for drug discovery lies in their often unique selectivity of their biological effects coupled with high potency. Toxic plant alkaloids were the first source of toxin-derived therapeutics, notably

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Section: Pain Therapeutics and Congenital Neurological Conditionsmentioning

confidence: 99%

Friends or Foes? Emerging Impacts of Biological Toxins

Clark

Casewell

Elliott

et al. 2019

Trends in Biochemical Sciences

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“…As the global antimicrobial resistance (AMR) crisis continues and exacerbates, the scientific community are engaged in not only trying to discover novel antimicrobials to treat antimicrobial‐resistant infections (Choi, Hwang, & Lee, 2014; Liu, Yang, et al, 2018) but also understand the drivers of resistance and potential reservoirs of resistant pathogens (Kotsanas et al, 2013; Li et al, 2018; Lowe et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Isolation of an antimicrobial‐resistant, biofilm‐forming, Klebsiella grimontii isolate from a reusable water bottle

et al. 2020

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A reusable water bottle was swabbed as part of the citizen science project “Swab and Send,” and a Klebsiella grimontii isolate was recovered on chromogenic agar and designated SS141. Whole‐genome sequencing of SS141 showed it has the potential to be a human pathogen as it contains the biosynthetic gene cluster for the potent cytotoxin, kleboxymycin, and genes for other virulence factors. The genome also contains the antibiotic‐resistant genes, blaOXY‐6‐4, and a variant of fosA, which is likely to explain the observed resistance to ampicillin, amoxicillin, and fosfomycin. We have also shown that SS141 forms biofilms on both polystyrene and polypropylene surfaces, providing a reasonable explanation for its ability to colonize a reusable water bottle. With the increasing use of reusable water bottles as an alternative to disposables and a strong forecast for growth in this industry over the next decade, this study highlights the need for cleanliness comparable to other reusable culinary items.

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“…Venoms produced by different species, such as scorpions, snakes, and spiders, are used as protection or to capture prey. Various peptides found in venom produced by scorpions have been reported to have broad-spectrum antimicrobial, anticancer, and anti-inflammatory activities [ 12 , 13 ]. Numerous scorpion venom antimicrobial peptides (AMPs) have been reported as various therapeutic agents because of their low resistance rate [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Scorpion-Venom-Derived Antimicrobial Peptide Css54 Exerts Potent Antimicrobial Activity by Disrupting Bacterial Membrane of Zoonotic Bacteria

Park

Kang

et al. 2020

Antibiotics

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Antibiotic resistance is an important issue affecting humans and livestock. Antimicrobial peptides are promising alternatives to antibiotics. In this study, the antimicrobial peptide Css54, isolated from the venom of C. suffuses, was found to exhibit antimicrobial activity against bacteria such as Listeria monocytogenes, Streptococcus suis, Campylobacter jejuni, and Salmonella typhimurium that cause zoonotic diseases. Moreover, the cytotoxicity and hemolytic activity of Css54 was lower than that of melittin isolated from bee venom. Circular dichroism assays showed that Css54 has an α-helix structure in an environment mimicking that of bacterial cell membranes. We examined the effect of Css54 on bacterial membranes using N-phenyl-1-naphthylamine, 3,3′-dipropylthiadicarbbocyanine iodides, SYTOX green, and propidium iodide. Our findings suggest that the Css54 peptide kills bacteria by disrupting the bacterial membrane. Moreover, Css54 exhibited antibiofilm activity against L. monocytogenes. Thus, Css54 may be useful as an alternative to antibiotics in humans and animal husbandry.

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Therapeutic Potential of a Scorpion Venom-Derived Antimicrobial Peptide and Its Homologs Against Antibiotic-Resistant Gram-Positive Bacteria

Cited by 40 publications

References 46 publications

Friends or Foes? Emerging Impacts of Biological Toxins

Friends or Foes? Emerging Impacts of Biological Toxins

Isolation of an antimicrobial‐resistant, biofilm‐forming, Klebsiella grimontii isolate from a reusable water bottle

Scorpion-Venom-Derived Antimicrobial Peptide Css54 Exerts Potent Antimicrobial Activity by Disrupting Bacterial Membrane of Zoonotic Bacteria

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