Therapeutic plasma proteins – application of proteomics in process optimization, validation, and analysis of the final product

Gašo-Sokač, Dajana; Kovač, Spomenka; Clifton, James R; Josić, Djuro

doi:10.1002/elps.201000641

Cited by 13 publications

(5 citation statements)

References 153 publications

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“…Neither HIC nor displacement chromatography have been routinely applied to the separation of proteins from human plasma. Elution‐modified displacement chromatography as an effective method for reversed‐phase separation of tryptic peptides as sample preparation for ESI MS was developed almost 10 years ago [], but this method did not find widespread acceptance. Ahrends et al [] recently compared displacement chromatography and gradient elution chromatography as the cation‐exchange step for sample preparation for ESI‐MS/MS.…”

Section: Discussionmentioning

confidence: 99%

Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode

et al. 2012

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Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately twenty years ago. This method was first used for the preparative purification of peptides and proteins. Recently, SDC in ion-exchange mode was also successfully used for enrichment of low abundance proteins from human plasma. In this paper, the use of SDC for the separation of plasma proteins in hydrophobic interaction mode is demonstrated. By use of two or more columns coupled in series during sample application, and subsequent elution of detached columns in parallel, additional separation of bound proteins was achieved. Further low-abundance, physiologically active proteins could be highly enriched and detected by ESI-MS/MS.

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Section: Discussionmentioning

confidence: 99%

Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode

et al. 2012

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“…protein-protein interaction, PTMs or by simple binding of small ligands, and these molecules can be also a potential drug target. The role of proteomics, especially of plasma membrane proteomics can be: (i) identification of new drug targets, (ii) investigation of the mechanism of actions of the existing ones, (iii) elucidation of the development of drug resistance and (iv) early detection of possible harmful side effects such as immunological reaction of protein drugs [149] or rejection in organ transplantation [79].…”

Section: Plasma Membrane Proteins As Possible Molecular Targets For Amentioning

confidence: 99%

Mammalian plasma membrane proteins as potential biomarkers and drug targets

2011

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Defining the plasma membrane proteome is crucial to understand the role of plasma membrane in fundamental biological processes. Change in membrane proteins is one of the first events that take place under pathological conditions, making plasma membrane proteins a likely source of potential disease biomarkers with prognostic or diagnostic potential. Membrane proteins are also potential targets for monoclonal antibodies and other drugs that block receptors or inhibit enzymes essential to the disease progress. Despite several advanced methods recently developed for the analysis of hydrophobic proteins and proteins with posttranslational modifications, integral membrane proteins are still under-represented in plasma membrane proteome. Recent advances in proteomic investigation of plasma membrane proteins, defining their roles as diagnostic and prognostic disease biomarkers and as target molecules in disease treatment, are presented.

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“…Cibachrom-blue is frequently used as an alternative to mAb for albumin removal from human plasma [57]. Immobilized heparin is frequently applied in plasma fractionation and for isolation of recombinant therapeutic proteins [34, 35, 37, 63], and SDC in affinity mode, as presented by Manseth et al [50], can be a valuable alternative for further optimization.…”

Section: Applications Of Sample Displacement Chromatographymentioning

confidence: 99%

“…Despite its importance as a starting material for diagnostic purposes and for discovery of new biomarkers, human plasma is still used as a valuable source for the isolation of therapeutic proteins [34, 35]. Large amounts of intravenous immunoglobulin (IVIG) and human serum albumin preparations originate from human plasma, and thousands of units of clotting factors and inhibitors are still needed in medicine as indispensable therapeutic agents [35–37].…”

Section: Introductionmentioning

confidence: 99%

“…Large amounts of intravenous immunoglobulin (IVIG) and human serum albumin preparations originate from human plasma, and thousands of units of clotting factors and inhibitors are still needed in medicine as indispensable therapeutic agents [35–37]. Fractionation of human plasma is a sixty-year old technology, and chromatography is widely used as an integral part of different production processes [34, 37, 38].…”

Section: Introductionmentioning

confidence: 99%

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Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures

Gajdošik¹,

Clifton²,

Josić³

2012

Journal of Chromatography A

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Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately twenty years ago. This method takes advantage of relative binding affinities of components in a sample mixture. During loading, there is a competition among different sample components for the sorption on the surface of the stationary phase. SDC was first used for the preparative purification of proteins. Later, it was demonstrated that this kind of chromatography can also be performed in ion-exchange, affinity and hydrophobic-interaction mode. It has also been shown that SDC can be performed on monoliths and membrane-based supports in both analytical and preparative scale. Recently, SDC in ion-exchange and hydrophobic interaction mode was also employed successfully for the removal of trace proteins from monoclonal antibody preparations and for the enrichment of low abundance proteins from human plasma. In this review, the principals of SDC are introduced, and the potential for separation of proteins and peptides in micro-analytical, analytical and preparative scale is discussed.

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Therapeutic plasma proteins – application of proteomics in process optimization, validation, and analysis of the final product

Cited by 13 publications

References 153 publications

Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode

Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode

Mammalian plasma membrane proteins as potential biomarkers and drug targets

Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures

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