Therapeutic mechanism of ginkgo biloba exocarp polysaccharides on gastric cancer

Xu, Anjian; Chen, Huasheng; Sun, Bu-Chan; Xiaoren, Xiang; Chu, Yun-Fei; Zha, Zheng-Jun; Jia, Ling-Chang

doi:10.3748/wjg.v9.i11.2424

Cited by 40 publications

(21 citation statements)

References 21 publications

(20 reference statements)

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“…Ginkgolide B (GB) is a component of ginkgo leaf, which has been widely used for treating asthma, cough and enuresis [22,23]. Previous studies have shown that GB has beneficial effects on endothelial cell protection [24] as well as cancer therapy [25][26][27]. However, whether GB may have an anti-bladder cancer effect is not known.…”

Section: Introductionmentioning

confidence: 99%

Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

Zhi

Pan

Shen

et al. 2016

Cell Physiol Biochem

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Background/Aims: Ginkgolide B (GB) is currently used as an anticancer drug for treatment of some malignant cancers. However, whether it may have therapeutic effects on bladder cancer remains unknown. Here, we studied the effects of GB on bladder cancer cells. Methods: Bladder cells were treated with different doses of GB, and the effects on ZEB1 and microRNA-223-3p (miR-223-3p) were analyzed by RT-qPCR and/or Western blot. Prediction of a regulatory relationship between miR-93 and 3'-UTR of Beclin-1 mRNA was performed by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Results: We found that GB dose-dependently decreased ZEB1 protein, but not mRNA, in bladder cancer cells, resulting in suppression of cell invasion. Moreover, in bladder cancer cells, GB dose-dependently decreased the levels of miR-223-3p, which suppressed the protein translation of ZEB1 through binding to 3'-UTR of ZEB1 mRNA. Overexpression of miR-223-3p decreased ZEB1 protein, while depletion of miR-223-3p increased ZEB1 protein in bladder cancer cells. Conclusion: GB inhibits bladder cancer cell invasiveness through suppressing ZEB1 protein translation via upregulating miR-223-3p.

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Section: Introductionmentioning

confidence: 99%

Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

Zhi

Pan

Shen

et al. 2016

Cell Physiol Biochem

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“…5 Recent studies conducted with various molecular, cellular, and whole animal models have revealed that leaf extracts of Ginkgo biloba may have anticancer (chemopreventive) properties that are related to their antioxidant, antiangiogenic, and gene or protein regulatory actions. [6][7][8][9] Cell line studies suggest that Ginkgo may reduce the growth of human breast cancer cells, perhaps through its action on the peripheral-type benzodiazepine pathway that is important in steroid hormone regulation, 10 and it has significant antiproliferative and cytotoxic effects on human hepatocellular carcinoma cells. 11 In vivo experiments suggest that Ginkgo may promote apoptosis of cancer cells by caspase-3/8 activation in oral cancer cells in rats.…”

Section: Introductionmentioning

confidence: 99%

Ginkgo May Sensitize Ovarian Cancer Cells to Cisplatin

et al. 2012

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Ginkgolide B (GB), the primary active component of Ginkgo biloba extracts, may have antitumor properties. The objective of this study was to determine the effects and possible mechanisms of GB in ovarian cancer cells. In this study, human ovarian cancer cell lines (SKOV3 and CAOV3) were treated with different concentrations of GB alone or in combination with Cis-diaminodichloroplatinum (CDDP). An MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed to determine cell viability. The apoptosis rates of cells were measured by flow cytometric analysis. The expression of apoptosis-associated and proliferation-associated proteins was detected by Western blot. The cytotoxicity of GB was analyzed using a lactate dehydrogenase assay. Treatment with 100 µM GB for 3 days significantly inhibited SKOV3 and CAOV3 cell proliferation by 57.3% and 63.1% compared with control cells, respectively, as determined by MTT assay. Similarly, the apoptotic cell population was increased when treated with GB in a dose-dependent manner both in SKOV3 and CAOV3 cells. These effects were characterized by the upregulation of p21, p27, cleaved capase-3, and cleaved caspase-8 and downregulation of cyclin D1. In addition, a combined treatment of low concentrations of GB and CDDP showed an additive effect on the inhibition of SKOV3 cell proliferation. Furthermore, GB had significantly less cytotoxicity than CDDP in normal human ovarian surface epithelial cells. This study suggests that GB can be proposed as an effective antiproliferative and apoptosis-inducing agent with interesting translational application in ovarian cancers, used in addition to conventional chemotherapy.

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“…Traditional cytotoxic drugs are powerful enough to kill tumor cells, but they come with high toxicity, especially with regards to immune and hemotologic function [30] . Thus, many doctors avoid using these kinds of drugs to their patients.…”

Section: H U M O R a L I M Mu N I T Y I S C R I T I C A L T O H O S Tmentioning

confidence: 99%

Effects of a Shuangling Fuzheng anticancer preparation on the proliferation of SGC-7901 cells and immune function in a cyclophosphamide-treated murine model

Huasheng¹,

Chen²,

Jue³

et al. 2007

WJG

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AIM:To study the inhibitory effects of a Shuangling Fuzheng anticancer preparation (SFAP) on the human gastric cancer cell line SGC-7901 in vitro as well as its immune-modulated effects in a cyclophosphamide-treated murine model. METHODS:MTT experiments and immunocytochemistry ABC experiments were performed for detecting the proliferation of SGC-7901 cells in vitro and protein expression of c-myc. The staphylococcal protein A (SPA) rosette test was utilized for measuring the ratio of T-lymphocyte subsets from peripheral blood in a cyclophosphamide-treated murine model. Enzymelinked immunosorbant assay (ELISA) was performed for measuring the levels of serum sIL-2R in treated mice, while immunoturbidimetry was used for measuring the levels of immunoglobulins (Ig).

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Therapeutic mechanism of ginkgo biloba exocarp polysaccharides on gastric cancer

Cited by 40 publications

References 21 publications

Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

Ginkgo May Sensitize Ovarian Cancer Cells to Cisplatin

Effects of a Shuangling Fuzheng anticancer preparation on the proliferation of SGC-7901 cells and immune function in a cyclophosphamide-treated murine model

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