Therapeutic Implication of Honey against Chronic Carbon Tetrachloride-Induced Liver Injury via Enhancing Antioxidant Potential and Maintenance of Liver Tissue Architecture

Abstract

The current study was undertaken to examine the possible hepatoprotective effect of natural honey against carbon tetrachloride (CCl 4)-induced liver injury in mice. A significant increase in the serum aminotransferases (ALT and AST) and alkaline phosphatase activity was noticed in mice exposed to CCl 4. In addition to this, a significant decrease in total antioxidant capacity and antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase) was observed in CCl 4-induction group. However, treatment with honey (400 mg/kg b.w, 4 times/ week) clearly demonstrates significant hepatoprotective activities by lowering the liver marker enzymes towards the normal reference range and restores the antioxidant enzyme levels(p<0.05). The effect of CCl 4 was also noticed microscopically by alteration in liver tissue architecture. The administration of liver toxicant causes, hemorrhage, congestion, necrosis, edema and remarkable blood vessel dilation. Moreover, honey exhibited protective action against this haloalkane in tissue architecture as the severity of liver tissue alteration was significantly reduced (p<0.05). The expressional pattern of P53 protein in groups treated with CCl 4 only as well as honey plus CCl 4 was statistically insignificant. In conclusion, this study reveals that natural honey has a remarkable protective effect against CCl 4-induced liver toxicity at antioxidant enzyme, histological and protein expression level.

A multidisciplinary approach to the antioxidant and hepatoprotective activities of Arbutus pavarii Pampan fruit; in vitro and in Vivo biological evaluations, and in silico investigations

“…The reduction in internal protective enzymatic and non-enzymatic antioxidant levels results in serious redox states and oxidation of cellular biomolecules, leading to acute and chronic liver disorders. 15 , 16 Therefore, external antioxidants supplemented in the diet or nutritional products are very important, especially for those with oxidative-related degenerative disorders or who live in areas where external oxidative stress factors are dominant. 17 , 18 In that context, most of the liver supports available on the market are antioxidant plant-based natural products.…”