Therapeutic Efficacy of “Nubiotics” against Burn Wound Infection by
            <i>Pseudomonas aeruginosa</i>

Dale, R.M.K.; Schnell, Glen; Wong, Jonathan P.

doi:10.1128/aac.48.8.2918-2923.2004

Cited by 46 publications

(40 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Induction of bacterial abscesses was carried out using a method described by Dale et al (2004) with some modifications that the mice were shaved on the abdomen rather than the dorsal area. Exactly 100 μl of S. aureus and P. aeruginosa broth were then injected subcutaneously at the shaved abdominal area.…”

Section: Grouping and Treatment Allocationmentioning

confidence: 99%

Effect of crude root extract from Synadenium glaucescens on selected bacterial infections in albino mice (Mus musculus)

Max¹,

Mwageni²,

Bakari³

2014

J. Med. Plants Res.

View full text Add to dashboard Cite

Studies were carried out to investigate the effect of root extract from a tropical plant Synadenium glaucescens against two pathogenic species of bacteria in mice. A total of 120 mice were used in two experiments involving two bacterial species, Staphylococcus aureus and Pseudomonas aeruginosa. In each experiment, 60 mice were randomly divided into 6 groups with or without bacterial infection and with or without root extract. Clinical signs and survival of the mice were monitored; skin, kidneys and livers were examined for gross lesions, histopathological changes and bacterial counts. Results indicated that mice infected with the two bacteria and treated with the root extract from S. glaucescens had significantly less (P < 0.05) severe skin lesions compared to mice from the untreated groups. Histopathological examination of liver and kidney tissues showed hydropic degeneration and inflammatory reaction in uninfected groups receiving the extracts. Protein in tubular lumens, desquamated tubular epithelium and necrosis around central veins were observed in livers of mice infected with S. aureus and treated with the extract at 50 mg/kg body weight. It is concluded that root extract from S. glaucescens had significant antibacterial activity against the tested bacteria. Histopathological changes in the kidneys and livers of mice which received the extract alone suggested that high doses of the tested extract could be harmful to the mice. Further studies are needed to find out optimum dosage and whether the extract is harmful to other organs.

show abstract

Section: Grouping and Treatment Allocationmentioning

confidence: 99%

Effect of crude root extract from Synadenium glaucescens on selected bacterial infections in albino mice (Mus musculus)

Max¹,

Mwageni²,

Bakari³

2014

J. Med. Plants Res.

View full text Add to dashboard Cite

show abstract

“…Figure 1A to D shows Masson trichrome-stained histological sections of healthy mouse skin and skin that was burned for 10, 30, and 60 s. As can be seen, the 30-and 60-s burns have channels in the dermis that were not formed in the 10-s burns, and we believed that bacteria applied to the surface could more easily penetrate these channels, thus gaining a foothold in the burn in order to multiply. Some reports in the literature involve the injection of bacteria into or beneath the burned tissue rather than their application to the surface (6,19). Since the chitosan acetate bandage is a superficial antimicrobial dressing, we preferred to use the more natural mode of surface contamination.…”

Section: Resultsmentioning

confidence: 99%

Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns

Tegos

Burkatovskaya

Castaño

et al. 2009

Antimicrob Agents Chemother

117

View full text Add to dashboard Cite

An engineered chitosan acetate bandage preparation (HemCon) is used as a hemostatic dressing, and its chemical structure suggests that it should also be antimicrobial. We previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal infections. Wound healing was also stimulated. In the present study, we asked whether a chitosan acetate bandage could act as a topical antimicrobial dressing when it was applied to third-degree burns in mice contaminated with two of these bacterial species (P. aeruginosa and P. mirabilis). Preliminary experiments established the length of burn time and the number of bacteria needed to produce fatal infections in untreated mice and established that the chitosan acetate bandage could adhere to the infected burn for up to 21 days. In the case of P. aeruginosa infections, the survival rate of mice treated with the chitosan acetate bandage was 73.3% (whereas the survival rate of mice treated with a nanocrystalline silver dressing was 27.3% [P ‫؍‬ 0.0055] and that of untreated mice was 13.3% [P < 0.0002]). For P. mirabilis infections, the comparable survival rates were 66.7%, 62.5%, and 23.1% respectively. Quantitative bioluminescent signals showed that the chitosan acetate bandage effectively controlled the growth of bacteria in the burn and prevented the development of systemic sepsis, as shown by blood culture. These data suggest that chitosan acetate bandage is efficacious in preventing fatal burn infections.

show abstract

“…A third degree burn skin infection model was developed in tree shrews by using S. aureus (Dale et al, 2004). Briefly, tree shrews were anesthetized with 90 mg/kg ketamine HCl and 10 mg/kg xylazine.…”

Section: Burned Skin Infection Modelmentioning

confidence: 99%

Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents

Lee²,

Zhang³

2013

Zoological Research

View full text Add to dashboard Cite

Animal models are essential for the development of new anti-infectious drugs. Although some bacterial infection models have been established in rodents, small primate models are rare. Here, we report on two bacterial infection models established in tree shrew (Tupaia belangeri chinensis). A burnt skin infection model was induced by dropping 5×106 CFU of Staphylococcus aureus on the surface of a wound after a third degree burn. This dose of S. aureus caused persistent infection for 7 days and obvious inflammatory response was observed 4 days after inoculation. A Dacron graft infection model, 2×10 6 CFU of Pseudomonas aeruginosa also caused persistent infection for 6 days, with large amounts of pus observed 3 days after inoculation. These models were used to evaluate the efficacy of levofloxacin (LEV) and cefoperazone (CPZ), which reduced the viable bacteria in skin to 4log 10 and 5log 10 CFU/100 mg tissue, respectively. The number of bacteria in graft was significantly reduced by 4log 10 CFU/mL treatment compared to the untreated group (P<0.05). These results suggest that two bacterial infection models were successfully established in tree shrew using P. aeruginosa and S. aureus. In addition, tree shrew was susceptible to P. aeruginosa and S. aureus, thus making it an ideal bacterial infection animal model for the evaluation of new antimicrobials.

show abstract

Therapeutic Efficacy of “Nubiotics” against Burn Wound Infection by Pseudomonas aeruginosa

Cited by 46 publications

References 11 publications

Effect of crude root extract from Synadenium glaucescens on selected bacterial infections in albino mice (Mus musculus)

Effect of crude root extract from Synadenium glaucescens on selected bacterial infections in albino mice (Mus musculus)

Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns

Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents

Contact Info

Product

Resources

About