Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats

Abstract

Introduction:We have previously demonstrated that sequentially extracted methanolic fractions of Boerhaavia diffusa (Linn.) showed marked antioxidant, antidiabetic and oxidative-DNA damage protective properties in vitro. The present study was undertaken to evaluate the beneficial effects of B.diffusa (Linn.) methanolic root extract and its partially purified bioactive fraction on streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Methods: The diabetic rats were treated for fourteen weeks either with methanolic extract of B. diffusa root (D-MT1, D-MT2, and D-MT3 : doses of 50, 150, and 300 mg/rat/day, respectively), partially isolated bioactive fraction (D-BT: 0.5 mg/rat/day), or glibenclamide (D-GT: 0.5 mg/rat/day). Results: The level of fasting blood glucose (FBG) and glycated hemoglobin (HbA1c) were significantly alleviated in D-MT-and D-BTtreated groups after fourteen weeks of administration. Moreover, plasma lipid profile, free fatty acids (FFAs), phospholipids (PLs), HMG-CoA reductase (HMG-R) activity, conjugated diene (CD), lipid hydroperoxide (LOOH), and malondialdehyde (MDA) were also markedly ameliorate d in all treatment groups. In addition, the activity of antioxidant enzymes, e.g., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gred), and glutathione-S-transferase (GST), were also significantly restored by D-MT -and D-BT -treated groups. Furthermore, histologically, all the unseemly features of nephropathy were extensively regressed and normalized by the administration of B. diffusa and its bioactive fraction. Conclusions: Our results demonstrate a strong antidiabetic and hypolipidemic impact of B. diffusa extract an ideal alternative therapeutic agent in the prevention and treatment of diabetes as well as diabetes-linked hyperlipidemia.Cite this article : Akhter F, Alvi S S, Ahmad P, Iqbal D, Alshehri B M, Khan M S. Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats. Biomed. Res. Ther.; 6(7):3293-3306.

Amelioration of neuropilin-1 and RAGE/matrix metalloproteinase-2 pathway-induced renal injury in diabetic rats by rosuvastatin

“…In vivo experimental design STZ, RT and GB preparation Fresh STZ (60 mg/kg b.w. of rat) was dissolved in citrate buffer (pH 4.5) as reported earlier [23,24]. The doses of RT [25] and the reference drug GB (10 mg/Kg B.W./rat/day) were prepared in 10% DMSO as per recent reports [23,24].…”

“…The animals were assigned to four groups and DM was induced as described [23,24]. Briefly, fasted animals were injected intraperitonially (i.p.)…”

“…The level of FBG was measured by an enzymatic "Autospan kit" following the manufacturer's instructions as described earlier [23]. The level of HbA1c was determined using a GenX HbA1c automated immunoassay kit supplied by Proton Biologicals India Pvt.…”

“…Ltd., India. The assay principle was based on the direct interaction of antigen and antibody, and the percentage of HbA1c was calculated by measuring the absorbance of lyophilized calibrators at 660 nm with an Eppendorf BioSpectrophotometer [23].…”

Geno-Protective, Free Radical Scavenging and Antimicrobial Potential of Hyptis suaveolens Methanolic Fraction: An In-Vitro Study

“…Previously, it has been reported that plants are the good source of natural bioactive compounds against vast variety of diseases [10]. Medicinal plants or their bioactive fractions were also reported as potent antioxidant, antimicrobial, geno-protective, hypolipidemic, antidiabetic, neuroprotective agents [11][12][13][14][15][16][17][18][19][20][21][22][23]. Studies also revealed the antioxidant and antimicrobial effects of several plant species, such as Nandina domestica [24].…”