Therapeutic efficacy of autologous platelet‐rich plasma and polydeoxyribonucleotide on female pattern hair loss

Abstract

Autologous platelet-rich plasma (PRP) exerts positive therapeutic effects on hair thickness and density in patients with pattern hair loss. The aim of our study was to evaluate the efficacy of intra-perifollicular autologous PRP and polydeoxyribonucleotide (PDRN) injections in treating female pattern hair loss (FPHL). Twenty FPHL patients were treated with a single session of PRP injection, followed by 12 sessions of PDRN intra-perifollicular injection, along the scalp at weekly intervals. Additionally, another 20 FPHL patients were treated with 12 sessions of PDRN injection only. Meanwhile, one half of the backs of two rabbits was injected with the PRP preparation, while the other half was injected with phosphate buffered saline as a control. Tissue samples from the rabbits were analyzed by real-time polymerase chain reaction and Western blotting. Compared with baseline values, patients treated with PRP and PDRN injections exhibited clinical improvement in mean hair counts (23.2 ± 15.5%; p < 0.001) and mean hair thickness (16.8 ± 10.8%; p < 0.001). In addition, patients treated with the 12 sessions of intra-perifollicular PDRN injection alone also showed clinical improvement in mean hair counts (17.9 ± 13.2%; p < 0.001) and mean hair thickness (13.5 ± 10.7%; p < 0.001). Comparison analyses between the two groups revealed that combined therapy with PRP and PDRN induces greater improvement in hair thickness than treatment with PDRN therapy alone (p = 0.031), but not in hair counts (p > 0.05). The pilot animal study revealed significant up-regulation of WNT, platelet-derived growth factor, and fibroblast growth factor expression in rabbit skin treated with the PRP preparation, compared with control skin. In conclusion, intra-perifollicular injections of autologous PRP and/or PDRN generate improvements in hair thickness and density in FPHL patients.

Therapeutic Efficacy of 1,927-nm Fractionated Thulium Laser Energy and Polydeoxyribonucleotide on Pattern Hair Loss

“…Our study group previously reported that repetitive intra-perifollicular injections of PDRN results in significant improvements in hair counts and thickness in female patients with PHL. 7 In review of our present results, we suggest that PDRN stimulates wound healing after thulium laser treatment, alleviates the potential side effects of laser therapy, and promotes hair follicle restoration.…”

“…The lens of the camera was positioned at the "V" point along the vertex of the scalp, where the mid-sagittal and coronal planes intersected. 6,7 Thereafter, relative values for hair counts and thickness Original Article…”

“…As described in a previous report, 6,7 digital images of the scalp and hairs were captured at 1× and 40× magnification using a computerized, hand-held USB camera PT system (Follioscope ® ; LeedM Corporation, Seoul, Korea) for objective evaluation at baseline and at one week after the final treatment. The lens of the camera was positioned at the "V" point along the vertex of the scalp, where the mid-sagittal and coronal planes intersected.…”

“…1 To do so, along with oral medications of finasteride or dutasteride, topical agents of minoxidil and alfatradiol, and hair transplantation, injections of tissue activators, anti-oxidants, and autologous platelet-rich plasma concentrations have also been used to treat PHL. [1][2][3][4][5][6][7] Commonly used tissue activators and anti-oxidants in PHL patients include polydeoxyribonucleotide (PDRN), placental extracts, zinc sulfate, biotin, and nicotinamide. 6,7 Laser/light-based devices have also been utilized as additional or alternative treatment modalities for treating PHL, alopecia areata, and scarring alopecia.…”

“…[1][2][3][4][5][6][7] Commonly used tissue activators and anti-oxidants in PHL patients include polydeoxyribonucleotide (PDRN), placental extracts, zinc sulfate, biotin, and nicotinamide. 6,7 Laser/light-based devices have also been utilized as additional or alternative treatment modalities for treating PHL, alopecia areata, and scarring alopecia. These devices have been shown to improve blood supply to hair follicles and to up-regulate expression of signaling molecules and growth factors.…”