Therapeutic effects of silver nanoparticle and L-carnitine on aerobic vaginitis in mice: an experimental study

Abstract

Introduction: Aerobic vaginitis (AV) is a type of vaginal infection that occurs at the reproductive age of women. In this study, we aimed to study the possible anti-AV therapeutic effects of silver nanoparticles (AgNPs) and L-carnitine (LC) in the mouse model. Methods: AV model was established by intra-vaginal inoculation of 108 CFU/mL Staphylococcus aureus and Escherichia coli (1:1) in adult NMRI mice. Susceptibilities of the bacteria were examined against AgNPs by inhibitory concentration (IC-50 and IC-90) and minimum biofilm inhibitory concentration (MBIC- 90) methods. The regimens therapy was intra-vaginal inoculation of AgNPs at MBIC- 90 and a daily injection of 250 mg/kg LC for two weeks. Mice were classified into healthy (control) and AV groups and then treated by LC, AgNPs, and AgNPs + LC. The vaginal smears were taken daily and tissue sections were prepared using the hematoxylin and eosin (H & E) method. Results: Minimum inhibitory concentrations (MICs) of AgNPs for E. coli, S. aureus, and their mixture were 250, 125, and 500 ppm, and their MBIC-90% were 500, 250, and 1000 ppm, respectively. The estrus cycle of mice treated with co-administration of AgNPs and LC was similar to the control group (P < 0.05). The results of histology also showed that infected mice were treated with AgNPs and LC, simultaneously. Conclusion: Single bacteria are more sensitive than their mixed model to these NPs. Co-administration of AgNPs as an antibacterial agent and LC as an antioxidant agent can treat AV in the infected mice.

Therapeutic effects of L‐arginine, L‐carnitine, and mesenchymal stem cell‐conditioned medium on endometriosis‐induced oocyte poor quality in an experimental mouse model

“…Then, each of these main groups was divided into four subgroups; Mice without injection did not receive treatment (control group). Mice were injected intraperitoneally daily with 250 mg/kg body weight of LA for 14 days (according to a pilot study and previous study) 15 Mice were injected intraperitoneally daily with 250 mg/kg body weight of LC for 14 days 16 Mice were injected with tail vein with a final volume of 100 μL of CM of bone marrow mesenchymal stem cells (BMSCs) for 14 days intravenous (IV) (all treatments were given at 10 a.m.) 17 …”

“…Mice were injected intraperitoneally daily with 250 mg/kg body weight of LC for 14 days. 16 4. Mice were injected with tail vein with a final volume of 100 μL of CM of bone marrow mesenchymal stem cells (BMSCs) for 14 days intravenous (IV) (all treatments were given at 10 a.m.).…”

Changes in vaginal secretion lipidom as a result of therapy in patients with vulvovaginal atrophy

“…Исследование, опубликованное в журнале женского здоровья, показало, что добавки ALCAR улучшают сексуальную функцию, включая возбуждение и любрикацию, у женщин с гипоактивным расстройством полового влечения [21]. Было показано, что ALCAR может усиливать функцию нервов и кровеносных сосудов, участвующих в сексуальной реакции, он может иметь потенциальное терапевтическое применение при лечении вульводинии, хронического болевого расстройства, поражаю щего вульву или наружные половые органы у женщин [22].…”