Therapeutic effects of mesenchymal stem cells-conditioned medium derived from suspension cultivation or silymarin on liver failure mice

Molaei, Sedigheh; Amiri, Fatemeh; Salimi, Rasoul; Ferdowsi, Shirin; Bahadori, Marzie

doi:10.1007/s11033-022-07785-4

Cited by 6 publications

(1 citation statement)

References 54 publications

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“…Because of its therapeutic efficiency and good bioavailability, extracts from the fruits of S. marianum are most often used as various medical oral forms (pills, capsules, syrups, etc.) for recovery of the functional condition of the liver (Mengesha et al, 2021;Molaei et al, 2022). Despite the fact that S. marianum had proved itself as a highly effective medicinal plant for various liver pathologies, development of medicinal drugs requires further research.…”

Section: Discussionmentioning

confidence: 99%

Treatment of animals with fatty liver disease using a drug based on the seeds of Silybum marianum

Vlizlo,

Prystupa,

Slivinska

et al. 2023

Regul. Mech. Biosyst.

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Medicinal plants are a source of various therapeutic preparations. Therefore, the aim of our work was to prepare a liposomal drug from extract from the seeds of Silybum marianum (L.) Gaertn., adding tocopherol acetate, lecithin, squalene, and Tween 80. The drug was used on the laboratory animals (rats) intramuscularly to measure the efficacy of treatment of experimentally modeled toxic fatty liver disease. The fatty infiltration of the liver in the rats was caused by tetrachloromethane (ССl4). The efficacy of the liposomal drug based on the extract from S. marianum seeds was studied on 25 animals in which the liver pathology had been caused by 50% oil solution of ССl4, administered in the dose of 5 mL per kg of body weight. The diseased rats were divided into five groups, each consisting five animals. Animals of the four experimental groups – first, second, third, and fourth - received the drug intramuscularly in the doses of 0.05, 0.25, 0.50, and 1.50 mL/kg of body weight three times every two days, respectively. At the same time, the control rats received three-time intramuscular injection of physiological solution in the dose of 0.5 mL/kg of body weight. Treatment of the animals with fatty liver disease by injections of the drug based on the extract from S. marianum seeds normalized the general condition, significantly improved the functions and structure of the liver. Biochemical studies of blood serum of the sick animals after the treatment revealed increase in albumin content, which may suggest reduction of the protein-synthesizing function of the liver. The normalization of the bile-forming and bile-excreting functions of the liver, and also elimination of cholestasis were evidenced by reduced contents of bile acids and total bilirubin and increased total cholesterol in the blood serum of the rats. After treating the animals with the created drug, we saw decrease in the activity of the liver-indicator enzymes (aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and glutamate dehydrogenase) in the blood serum, which is a sign of recovery of the structure of hepatocytes and elimination of cytolysis. Histological studies of the liver of the treated rats confirmed the positive effect of the liposomal drug on the organ’s structure. In the future studies, we plan to test this combination of agents in treatment of agricultural and domestic animals with liver pathologies.

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Section: Discussionmentioning

confidence: 99%

Treatment of animals with fatty liver disease using a drug based on the seeds of Silybum marianum

Vlizlo,

Prystupa,

Slivinska

et al. 2023

Regul. Mech. Biosyst.

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Influential factors for optimizing and strengthening mesenchymal stem cells and hematopoietic stem cells co-culture

Shirdare,

Amiri,

Samiee

et al. 2024

Mol Biol Rep

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Mesenchymal stem cells [MSCs] and Hematopoietic stem cells [HSCs] are two types of bone marrow stem cells that could proliferate and differentiate into different cell lineages. HSCs interact with MSCs under the protective conditions, called niche. Numerous studies have indicated supportive effects of MSCs on HSCs proliferation and differentiation. Furthermore, HSCs have many clinical applications and could treat different hematologic and non-hematologic diseases. For this purpose, there is a need to perform in vitro studies to optimize their expansion. Therefore, various methods including co-culture with MSCs are used to address the limitations of HSCs culture. Some parameters that might be effective for improving the co-culture system, such as MSC paracrine pro le, scaffolds, hypoxia, culture medium additives, and the use of various MSC sources, have been examined in different studies. In this article, we investigated the potential factors for optimizing HSCs/ MSCs co-culture. It might be helpful to apply a suitable approach for providing high quality HSCs and improving their therapeutic applications in the required elds.

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Mesenchymal stem cell-conditioned medium prevents inflammation-induced liver and lung damage in septic mice

Shahi,

Khosrojerdi,

Soudi

et al. 2024

International Immunopharmacology

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Therapeutic effects of mesenchymal stem cells-conditioned medium derived from suspension cultivation or silymarin on liver failure mice

Cited by 6 publications

References 54 publications

Treatment of animals with fatty liver disease using a drug based on the seeds of Silybum marianum

Treatment of animals with fatty liver disease using a drug based on the seeds of Silybum marianum

Influential factors for optimizing and strengthening mesenchymal stem cells and hematopoietic stem cells co-culture

Mesenchymal stem cell-conditioned medium prevents inflammation-induced liver and lung damage in septic mice

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