Therapeutic Effects of Bone Marrow-Derived Mesenchymal Stem Cells Engraftment on Bleomycin-Induced Lung Injury in Rats

Zhao, Feiyan; Zhang, Y.F.; Liu, Y.G.; Zhou, Jun; Li, Z.K.; Wu, Changgui; Qi, Hao-wen

doi:10.1016/j.transproceed.2008.01.080

Cited by 115 publications

(97 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Unlike classical intravenous or intratracheal delivery methods [5][6][7][8][9] , intrapleurally delivered MSCs were found to be distributed only to the pleurae and the pleural cavity, providing a promising cell-based therapeutic strategy for primary and secondary pleural diseases.…”

Section: Discussionmentioning

confidence: 98%

“…The animals used in this study were specific-pathogen-free male Sprague-Dawley rats (6-8 weeks old; Slac Laboratory Isolation, purification, and expansion of MSCs MSCs were isolated from rat bone marrow as described in previous studies [8] . In brief, whole marrow was flushed from the tibias and femurs of Sprague-Dawley rats (4-weeks old, male) with ice-cold Dulbecco's modified Eagle's medium/F12 (DMEM/F12; Gibco, Carlsbad, CA, USA).…”

Section: Animal Carementioning

confidence: 99%

“…For labeling, MSCs were incubated for 4 h in DMEM/ F12 medium containing 50 μg/mL DAPI. Cells were washed 6 times with PBS and then dissociated into a single cell suspension with 0.25% trypsin/1 mmol/L EDTA for further analysis [8] .…”

Section: Dapi Labeling Of Mscs and Growth Characteristics Assaysmentioning

confidence: 99%

“…Recent studies, including one of our previous studies, have demonstrated that intravenous (iv) or intratracheal (it) delivery of bone marrow-derived mesenchymal stem cells (MSCs) participates in the improvement of various experimental models of lung injury, thus demonstrating that MSCs play an important role in the treatment of animals with acute lung injury [5][6][7][8][9][10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Intrapleural delivery of mesenchymal stem cells: a novel potential treatment for pleural diseases

Qin

Jian

et al. 2011

Acta Pharmacol Sin

View full text Add to dashboard Cite

Aim: To develop a method to deliver mesenchymal stem cells (MSCs) into the pleural cavity for the treatment of pleural diseases. Methods: MSCs were isolated from rat bone marrow of rats and labeled with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) or green fluorescent protein (GFP) using a lentiviral vector. Eighteen Sprague-Dawley (SD) rats were inoculated intrapleurally with 1×10 6 MSCs-DAPI. The distribution of the fluorescent cells was observed using fluorescent microscopy for the following 30 d. Another 12 rats inoculated intrapleurally with 1×10 6 MSCs-GFP were observed for 14 d. Results: The isolated cells were typical MSC phenotypes and could differentiate into adipocytes, osteoblasts, and chondroblasts in vitro. Microscopic analysis revealed that the labeled cells adhered to the surface of the pleural cavity. The highest number of the labeled cells was found to be adhered to all specimens from the mediastinal pleura, but no labeled cells were detected in the lung parenchyma or other tissues/organs, such as the liver, kidney, spleen, and mesenterium. Incidentally, stomas were found in the mediastinal pleura. The recovered MSCs-GFP from the pleural cavity retained their ability to adhere and proliferate. Conclusion: We have established a novel method for intrapleural delivery of MSCs. The distribution of intrapleurally delivered MSCs was found to be limited to the pleurae and the pleural cavity, thereby providing us with a new approach to further investigation of the therapeutic roles of MSCs in pleural diseases.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Animal Carementioning

confidence: 99%

Section: Dapi Labeling Of Mscs and Growth Characteristics Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Intrapleural delivery of mesenchymal stem cells: a novel potential treatment for pleural diseases

Qin

Jian

et al. 2011

Acta Pharmacol Sin

View full text Add to dashboard Cite

show abstract

“…Mesenchymal stem cells (MSCs) have been shown to improve bleomycin-induced lung injury and fibrosis in animal models (Ortiz et al 2003;Rojas et al 2005;Cui et al 2007;Zhao et al 2008;Moodley et al 2009). Thus, transplanting MSCs may represent a promising strategy for limiting the progression of fibrosis, although more research is needed to understand the underlying mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Umbilical Cord Mesenchymal Stem Cells for Inhibiting Myofibroblastic Differentiation of Irradiated Human Lung Fibroblasts

Zhang

Zhu

Zhang

et al. 2015

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Radiation-induced lung injury (RILI) limits the benefits of radiotherapy in patients with lung cancer. Radiation-induced differentiation of lung fibroblasts to myofibroblasts plays a key role in RILI. Recent studies have shown that mesenchymal stem cells (MSCs) can protect against lung fibrosis and that Wnt/ β-catenin signaling is involved in fibrotic processes. In the present study, we explored the therapeutic potential of human umbilical cord MSCs (HUMSCs) for preventing radiation-induced differentiation of human lung fibroblasts (HLFs) to myofibroblasts. There are two advantages in the use of HUMSCs; namely, they are easily obtained and have low immunogenicity. Irradiated HLFs were co-cultured with HUMSCs. Expression of α-smooth muscle actin (α-SMA), a myofibroblast marker, was measured by Western blot analysis and immunohistochemistry. Irradiation (X-rays, 5 Gy) induced the differentiation of HLFs into myofibroblasts, which was inhibited by co-culture with HUMSCs. Irradiation also caused activation of the canonical Wnt/β-catenin signaling in HLFs, as judged by increased phosphorylation of glycogen synthase kinase 3β, nuclear accumulation of β-catenin, and elevated levels of Wnt-inducible signaling protein-1 (WISP-1) in the conditioned medium. However, co-culture with HUMSCs attenuated the radiation-induced activation of the Wnt/β-catenin signaling. We also measured the expression of FRAT1 that can enhance the Wnt/β-catenin signaling by stabilizing β-catenin. Co-culture with HUMSCs decreased FRAT1 protein levels in irradiated nHLFs. Thus, co-culture with HUMSCs attenuated the radiation-induced activation of Wnt/β-catenin signaling in HLFs, thereby inhibiting myofibroblastic differentiation of HLFs. Wnt/β-catenin signaling is a potential therapeutic target for limiting RILI in patients receiving radiotherapy for lung cancer.

show abstract

Pulmonary-Respiratory Medicine

2001

JAMA

View full text Add to dashboard Cite

Therapeutic Effects of Bone Marrow-Derived Mesenchymal Stem Cells Engraftment on Bleomycin-Induced Lung Injury in Rats

Cited by 115 publications

References 19 publications

Intrapleural delivery of mesenchymal stem cells: a novel potential treatment for pleural diseases

Intrapleural delivery of mesenchymal stem cells: a novel potential treatment for pleural diseases

Therapeutic Potential of Umbilical Cord Mesenchymal Stem Cells for Inhibiting Myofibroblastic Differentiation of Irradiated Human Lung Fibroblasts

Pulmonary-Respiratory Medicine

Contact Info

Product

Resources

About