Therapeutic effect of topical application of curcumin during treatment of radiation burns in a mini-pig model

Kim, Joong Sun; Park, Sunhoo; Jeon, Byung-Suk; Jang, Won-Seok; Lee, Sun-Joo; Son, Yeonghoon; Rhim, K S; Lee, Soong-In; Lee, Seung-Sook

doi:10.4142/jvs.2016.17.4.435

Cited by 26 publications

(24 citation statements)

References 39 publications

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“…Changes in basal cell density and epidermal depth were previously shown to correspond to gross skin morphology scores [13][14][15][16]. Epidermal thickness began to decrease 1 week after radiation-induced skin injury, which was evident 6-8 weeks after βor γ-irradiation.…”

Section: Jrprmentioning

confidence: 68%

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Comparison of Skin Injury Induced by β- and γ-irradiation in the Minipig Model

et al. 2017

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Background: The effects of radiation on tissues vary depending on the radiation type. In this study, a minipig model was used to compare the effects of β-rays from 166 Ho and γ-rays from 60 Co on the skin. Materials and Methods: In this study, the detrimental effects of βand γ-irradiation on the skin were assessed in minipigs. The histopathological changes in the skin from 1 to 12 weeks after exposure to 50 Gy of either β-(using 166 Ho patches) or γ-(using 60 Co) irradiation were assessed. Results and Discussion: The skin irradiated by β-rays was shown to exhibit more severe skin injury than that irradiated by γ-rays at 1-3 weeks post-exposure; however, while the skin lesions caused by β-rays recovered after 8 weeks, the γ-irradiated skin lesions were not repaired after this time. The observed histopathological changes corresponded with gross appearance scores. Seven days post-irradiation, apoptotic cells in the basal layer were detected more frequently in β-irradiated skin than in γ-irradiated skin. The basal cell density and skin thickness gradually decreased until 4 weeks after γand βirradiation. In β-irradiated skin lesions, and the density and thickness increased sharply back to control levels by 6-9 weeks. However, this was not the case in γ-irradiated skin lesions. In γ-irradiated skin, cyclooxygenase-2 (COX-2) was shown to be expressed in the epidermis, endothelial cells of vessels, and fibroblasts, while β-irradiated lesions exhibited COX-2 expression that was mostly limited to the epidermis. Conclusion: In this study, β-rays were shown to induce more severe skin injury than γ-rays; however, the β-rays-induced injury was largely repaired over time, while the γ-rays-induced injury was not repaired and instead progressed to necrosis. These findings reveal the differential effects of γand β-irradiation on skin and demonstrate the use of minipigs as a beneficial experimental model for studying irradiation-induced skin damage.

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Section: Jrprmentioning

confidence: 68%

“…The application of βor γ-rays to skin generally appears to trigger a linear loss of basal cells [13][14][15][16] and histological studies have demonstrated an early pyknosis of cells in the Fig. 4.…”

Section: Jrprmentioning

confidence: 99%

Comparison of Skin Injury Induced by β- and γ-irradiation in the Minipig Model

et al. 2017

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“… 23 – 26 Domestic swine were chosen due to the amount of tissue for analysis, similar skin characteristics to humans with regard to epidermal/dermal thickness, wound healing rates, and the propensity to develop hypertrophic scars following deep thermal injuries. 27 – 31 The goal of this study was to quantify the changes in the biomechanical properties, color and volume following saline (injection trauma), fat and ASCs injection of hypertrophic burn scar using an established hypertrophic burn scar model in domestic swine. 18 We hypothesized that the hypertrophic scars injected with fat and ASCs would improve over a 12-week period compared with untreated and saline-injected scar.…”

Section: Discussionmentioning

confidence: 99%

Effects of Autologous Fat and ASCs on Swine Hypertrophic Burn Scars: A Multimodal Quantitative Analysis

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Schwentker

Visscher

et al. 2017

Plastic and Reconstructive Surgery - Global Open

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Background:Hypertrophic scar formation is unpredictable and poorly understood, afflicting both the pediatric and adult populations. Treatment methods with conservative and invasive approaches have low rates of compliance and high rates of morbidity. The purpose of this study was to test a reproducible scar model and investigate a new technique of scar modification through the use of adipose- derived progenitor stromal cells (ASCs).Methods:Twenty thermal deep-partial thickness contact burns were created on the dorsum of three 8-week-old domestic swine and allowed to mature for 10 weeks. Scars were then injected with 2 cc saline, expanded autologous ASCs, or 2 cc fresh lipoaspirate and sampled at 2 week intervals up to 10 weeks postinjection. Volumetric analysis with a 3-D scanner, mechanical elasticity testing through negative pressure transduction, and standardized photography evaluation with Image J was performed. RNA sequencing was performed on scar tissue samples, cultured cells, and fresh lipoaspirate to determine relevant gene transcription regulation. Immunohistochemistry was used to verify expression level changes within the scars.Results:Volumetric analysis demonstrates a reduction in average scar thickness at 6 weeks when injected with ASCs (−1.6 cc3) and autologous fat (−1.95 cc3) relative to controls (−0.121 cc3; P < 0.05). A decrease in overall tissue compliance is observed with fat or ASC injection when compared with unburned skin at 8 weeks (35.99/37.94 versus 49.36 mm Hg × mm; P < 0.01). RNA sequencing demonstrates altered regulation of fibroblast gene expression and a decreased inflammatory profile when scars are injected with autologous fat/ASCs over controls.Conclusion:Early results suggest that autologous fat and/or ASCs may improve healing of hypertrophic scarring by altering the cellular and structural components during wound remodeling up to 20 weeks after injury. This may have beneficial applications in early treatment of large or cosmetically sensitive immature burn scars.

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“…In an animal model, daily topical application of curcumin showed to improve epithelial cell survival and recovery in irradiated skin, reducing the expression of cyclooxygenase-2 and nuclear factor-kappaB [88].…”

Section: Introductionmentioning

confidence: 99%

“…In animal models, daily curcumin topical application accelerated wound healing in irradiated mini-pigs, and the application of chrysin-curcumin-loaded nanofibers reduced the levels of IL-6, MMP-2, TIMP-1, TIMP-2, and iNOS gene expression in male rats, resulting in the acceleration of the healing process of surgical wounds [28,88]. Transdermally applied curcumin on surgical wounds on rats produced marked inhibition of H 2 O 2 -induced damage to keratinocytes and fibroblasts, while application of curcumin-oligochitosan nanoparticle complex or with application of oligochitosan coated curcumin-loaded-liposomes resulted in faster healing of surgical wounds in mice compared with controls [29,30].…”

Section: Introductionmentioning

confidence: 99%

Potential of Curcumin in Skin Disorders

et al. 2019

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Curcumin is a compound isolated from turmeric, a plant known for its medicinal use. Recently, there is a growing interest in the medical community in identifying novel, low-cost, safe molecules that may be used in the treatment of inflammatory and neoplastic diseases. An increasing amount of evidence suggests that curcumin may represent an effective agent in the treatment of several skin conditions. We examined the most relevant in vitro and in vivo studies published to date regarding the use of curcumin in inflammatory, neoplastic, and infectious skin diseases, providing information on its bioavailability and safety profile. Moreover, we performed a computational analysis about curcumin’s interaction towards the major enzymatic targets identified in the literature. Our results suggest that curcumin may represent a low-cost, well-tolerated, effective agent in the treatment of skin diseases. However, bypass of limitations of its in vivo use (low oral bioavailability, metabolism) is essential in order to conduct larger clinical trials that could confirm these observations. The possible use of curcumin in combination with traditional drugs and the formulations of novel delivery systems represent a very promising field for future applicative research.

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Therapeutic effect of topical application of curcumin during treatment of radiation burns in a mini-pig model

Cited by 26 publications

References 39 publications

Comparison of Skin Injury Induced by β- and γ-irradiation in the Minipig Model

Comparison of Skin Injury Induced by β- and γ-irradiation in the Minipig Model

Effects of Autologous Fat and ASCs on Swine Hypertrophic Burn Scars: A Multimodal Quantitative Analysis

Potential of Curcumin in Skin Disorders

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