Therapeutic effect of Prosopis strombulifera (LAM) BENTH aqueous extract on a murine model of cutaneous leishmaniasis

Lozano, Esteban; Germanó, María José; Troncoso, Mariana; Bustos, María Fernanda García; Gamarra-Luques, Carlos; Cargnelutti, Diego Esteban

doi:10.1016/j.jtcme.2021.08.009

Cited by 4 publications

(6 citation statements)

References 25 publications

(26 reference statements)

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“…Mice treated with HABTO or ICTX showed a splenic index similar to the MA-treated mice, exhibiting a significant reduction compared to the PBS group, where strong splenomegaly was observed (Figure B). Reducing the parasite burden is related to a decrease in the visceralization of the parasite, which manifests in a reduction in the splenic index …”

Section: Resultsmentioning

confidence: 99%

“…We measured weekly the RHLF swelling using a digital caliper (SCHWYZ, ED-10P) until the end of the protocol at week 10. To estimate lesion size, the value for the noninfected RHL was subtracted from each infected RHL . Footpad swelling results were represented in mm.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the procedures were approved (protocol approval no. 132/2018) by the local Institutional Animal Care and Use Committee of the School of Medical Science, Universidad Nacional de Cuyo, Mendoza, Argentina …”

Section: Methodsmentioning

confidence: 99%

“…The splenic index was calculated at week 10 using the following formula: Splenic index = (spleen weight/body weight) × 100. As an indicator of visceralization, the splenic index was used …”

Section: Methodsmentioning

confidence: 99%

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Antiparasitic Activity of Two Natural Terpenes from Salvia cuspidata against Leishmania amazonensis

et al. 2023

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Leishmaniasis is a neglected disease caused by flagellated parasites of the Leishmania genus affecting more than 10 million people worldwide. Current treatments for leishmaniasis involve the administration of poorly tolerated drugs with toxic side effects in patients. There is an imperative necessity for novel compounds to treat this disease. One of the most used strategies in the search for different antiparasitic compounds is the screening of purified plant molecules. The diterpenes 12-hydroxy-11,14-diketo-6,8,12-abietatrien-19,20-olide (HABTO) and 5-epi-icetexone (ICTX) isolated from Salvia cuspidata were shown to be effective against Leishmania amazonensis in vitro and in vivo. They displayed an antiproliferative effect against L. amazonensis promastigotes. They also induce an increase in ROS levels and affect the mitochondrial activity of parasites. HABTO and ICTX in an in vivo model of cutaneous leishmaniasis decrease footpad swelling, parasite load, and splenic index. Moreover, they induce significant reduction in the O.D. of total anti-Leishmania IgG and IgG1 subtype antibody responses against L. amazonensis compared to the PBS group but maintain high levels of IgG2a. This suggests that in HABTO-or ICTX-treated mice, there is a slowdown in the progression of the disease. These terpenes could be considered as possible novel antileishmanial agents against L. amazonensis and thus treat cutaneous leishmaniasis.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Antiparasitic Activity of Two Natural Terpenes from Salvia cuspidata against Leishmania amazonensis

et al. 2023

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“…14 Recent scientific studies demonstrated that P. strombulifera aqueous extract (PsAE), orally administered in drinking water, does not induce acute or subchronic toxicity in laboratory animals, 15 determines antitumoral effects in colorectal and melanoma tumors in C57BL6wt mice, 16 reduces atherogenesis in C57BL/6j ApoE-KO mice, 17 and delays cutaneous leishmaniasis caused by Leishmania amazoniensis in BALB/c mice. 18 Moreover, the complete secondary metabolites profile of PsAE was characterized by UHPLC-Q/Orbitrap/MS/MS including simple organic and phenolic acids, procyanidins, flavonoids and oxylipins. 16 Between the 26 phytochemicals identified, some of the more abundant are: catechin, kaempferol glucoside, myricitrin 3-O-glucoside, naringoside, rutin and xilonic acid.…”

Section: Introductionmentioning

confidence: 99%