Therapeutic effect of human amniotic epithelial cells in murine models of Hashimoto's thyroiditis and Systemic lupus erythematosus

Tan, Bing; Yuan, Weixin; Li, Jinying; Yang, Penghui; Ge, Zhen; Liu, Jia; Qiu, Chen; Zhu, Xiaolong; Qiu, Cong; Lai, Dongmei; Guo, Lihe; Wang, Liang; Yu, Luyang

doi:10.1016/j.jcyt.2018.04.001

Cited by 27 publications

(30 citation statements)

References 33 publications

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“…It is rather complicated to illustrate the deep mechanisms by which hAESCs suppress an excessive immune response. It was reported that hAESCs are capable of preventing macrophage and T cell infiltration into the lesion area and maintaining the proper portions of different T cell subtypes by modulating the cytokine concentration inside the microenvironment in certain autoimmune disorder models [ 134 , 135 ]. Actually, previous research has focused on the anti-inflammation effects of conditioned medium and cytokines from hAESCs.…”

Section: The Paracrine Effect Of Human Amniotic Epithelial Stem Cementioning

confidence: 99%

“…The latter is considered to be a pivotal subtype in inhibiting inflammation and encouraging tissue repair. Three types of cytokines related to anti-inflammation have been proven to be produced by hAESCs and amniotic tissue: (1) apoptosis inducers such as tumor necrosis factor-α (TNF-α), Fas ligand (FasL), and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); (2) macrophage migration inhibitors such as transforming growth factor-β (TGF-β) and macrophage migration-inhibitory factor (MIF); and (3) other antiangiogenic and anti-inflammatory proteins including interleukin-1 receptor antagonist (IL-1ra), interleukin-10 (IL-10), tissue inhibitors of metalloproteinase (TIMPs), collagen XVⅢ, and thrombopondin-1 [ 134 , 135 , 136 , 137 , 138 ]. Data indicate that these cytokines are critical in the hAESC- and amniotic-tissue-mediated anti-inflammation process.…”

Section: The Paracrine Effect Of Human Amniotic Epithelial Stem Cementioning

confidence: 99%

“…Based on their plasticity and immunomodulatory properties, hAESCs have been considered for treatment of a broad variety of conditions, including lung fibrosis [ 40 , 115 , 131 , 149 , 151 , 152 , 153 , 154 ], spinal cord injuries [ 60 ], Parkinson’s disease [ 64 ], Alzheimer’s disease [ 155 ], diabetes [ 69 ], wound healing [ 147 , 148 , 156 ], liver fibrosis [ 75 , 157 , 158 , 159 ], autoimmune uveitis [ 135 ], Hashimoto’s thyroiditis and systemic lupus erythematosus [ 134 ], and intrauterine adhesions [ 160 , 161 , 162 ]. Among these preclinical trials involving hAESCs, autoimmune diseases make up a large proportion, presumably because of the tremendous effect of immunomodulation.…”

Section: The Therapeutic Potential and Clinical Advances Of Human mentioning

confidence: 99%

“…In autoimmune uveitis models, hAESCs inhibit the infiltration of macrophages and modulate the balance of T-cell subtypes, leading to the improvement of both local and systemic cytokine environments. To be specific, treatment with hAESCs suppresses the level of IL-17, IFN-γ, and monocyte chemoattractant protein (MCP)-1; meanwhile, the expression of IL-10 is upregulated locally and systemically [ 135 ]. Additionally, Tan et al demonstrated the therapeutic efficacy of hAESCs both in localized and systemic autoimmune disease models.…”

Section: The Therapeutic Potential and Clinical Advances Of Human mentioning

confidence: 99%

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Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Qiu

Cui

et al. 2020

IJMS

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Perinatal stem cells have been regarded as an attractive and available cell source for medical research and clinical trials in recent years. Multiple stem cell types have been identified in the human placenta. Recent advances in knowledge on placental stem cells have revealed that human amniotic epithelial stem cells (hAESCs) have obvious advantages and can be used as a novel potential cell source for cellular therapy and clinical application. hAESCs are known to possess stem-cell-like plasticity, immune-privilege, and paracrine properties. In addition, non-tumorigenicity and a lack of ethical concerns are two major advantages compared with embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). All of the characteristics mentioned above and other additional advantages, including easy accessibility and a non-invasive application procedure, make hAESCs a potential ideal cell type for use in both research and regenerative medicine in the near future. This review article summarizes current knowledge on the characteristics, therapeutic potential, clinical advances and future challenges of hAESCs in detail.

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Section: The Paracrine Effect Of Human Amniotic Epithelial Stem Cementioning

confidence: 99%

Section: The Paracrine Effect Of Human Amniotic Epithelial Stem Cementioning

confidence: 99%

Section: The Therapeutic Potential and Clinical Advances Of Human mentioning

confidence: 99%

Section: The Therapeutic Potential and Clinical Advances Of Human mentioning

confidence: 99%

See 2 more Smart Citations

Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Qiu

Cui

et al. 2020

IJMS

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“…Evidence shows that hAECs can express typical stem cell markers such as octamer-binding transcription factor 4, stage-specific embryonic antigen 3, and NANOG and have the ability to differentiate into ectoderm, endoderm, and mesoderm lineage cells in vitro 20 , 21 . Based on their advantages of pluripotency, low immunogenicity 22 , 23 , tumorigenicity deficiency, and easily attainable without ethical concerns, hAECs have been widely used in stem cell therapy for a wide range of conditions, such as premature ovary failure 24 , 25 , Hashimoto’s thyroiditis and systemic lupus erythematosus 26 , orthopedic surgery 27 , corneal alkali injury 28 , spinal cord injury 29 , and liver disease 30 . However, the role of hAECs in IUAs has not been identified.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Human Amniotic Epithelial Cells in Rat Models of Intrauterine Adhesions

Bai

Liu

Yuan³

et al. 2020

Cell Transplant

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As a refractory fibrosis disease, intrauterine adhesions (IUAs) is defined as fibrosis of the physiological endometrium. Although hysteroscopic adhesiolysis is widely recommended as an effective treatment, prognosis and recurrence remain poor in severe cases. Recently, stem cell therapy has been promoted as a promising treatment for IUAs. The ability of human amniotic epithelial cells (hAECs), emerging as a new candidate for stem cell therapy, to treat IUAs has not been demonstrated. To study the potential effects of hAECs on IUAs, we created an IUA rat model using mechanical injury and injected cultured primary hAECs into the rats’ uteri. Next, we observed the morphological structure of endometrial thickness and glands using hematoxylin and eosin staining, and we detected extracellular-matrix collagen deposition using Masson staining. In addition, we performed immunohistochemical staining and reverse-transcription polymerase chain reaction (RT-PCR) to investigate potential fibrosis molecules and angiogenesis factors 7 d after hAECs transplantation. Finally, we detected estrogen receptor (ER) and growth factors via RT-PCR to verify the molecular mechanism underlying cell therapy. In the IUA rat models, endometrial thickness and endometrial glands proliferated and collagen deposition decreased significantly after hAEC transplantation. We found that during the recovery of injured endometrium, the crucial fibrosis marker transforming growth factor-β (TGF-β) was regulated and angiogenesis occurred in the endometrial tissue with the up-regulation of vascular endothelial growth factor. Furthermore, hAECs were shown to promote ER expression in the endometrium and regulate the inflammatory reaction in the uterine microenvironment. In conclusion, these results demonstrated that hAEC transplantation could inhibit the progression of fibrosis and promote proliferation and angiogenesis in IUA rat models. The current study suggests hAECs as a novel stem cell candidate in the treatment of severe IUA.

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A 3D‐Printed Dual Driving Forces Scaffold with Self‐Promoted Cell Absorption for Spinal Cord Injury Repair

Qiu,

Sun,

et al. 2023

Advanced Science

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Stem cells play critical roles in cell therapies and tissue engineering for nerve repair. However, achieving effective delivery of high cell density remains a challenge. Here, a novel cell delivery platform termed the hyper expansion scaffold (HES) is developed to enable high cell loading. HES facilitated self‐promoted and efficient cell absorption via a dual driving force model. In vitro tests revealed that the HES rapidly expanded 80‐fold in size upon absorbing 2.6 million human amniotic epithelial stem cells (hAESCs) within 2 min, representing over a 400% increase in loading capacity versus controls. This enhanced uptake benefited from macroscopic swelling forces as well as microscale capillary action. In spinal cord injury (SCI) rats, HES–hAESCs promoted functional recovery and axonal projection by reducing neuroinflammation and improving the neurotrophic microenvironment surrounding the lesions. In summary, the dual driving forces model provides a new rationale for engineering hydrogel scaffolds to facilitate self‐promoted cell absorption. The HES platform demonstrates great potential as a powerful and efficient vehicle for delivering high densities of hAESCs to promote clinical treatment and repair of SCI.

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Therapeutic effect of human amniotic epithelial cells in murine models of Hashimoto's thyroiditis and Systemic lupus erythematosus

Cited by 27 publications

References 33 publications

Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Therapeutic Effect of Human Amniotic Epithelial Cells in Rat Models of Intrauterine Adhesions

A 3D‐Printed Dual Driving Forces Scaffold with Self‐Promoted Cell Absorption for Spinal Cord Injury Repair

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