Therapeutic effect of dental pulp stem cell transplantation on a rat model of radioactivity-induced esophageal injury

Zhang, Chunwei; Zhang, Yichi; Feng, Zhenning; Zhang, Feifei; Liu, Zishuai; Sun, Xiaoli; Ruan, Mengting; Liu, Mingna; Jin, Shizhu

doi:10.1038/s41419-018-0753-0

Cited by 22 publications

(12 citation statements)

References 53 publications

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“…The animal experiment showed the possibility of human DPSC can decrease the RGF loss in glaucoma which elevated intraocular pressure [ 51 ]. It is even reported to treat radioactive esophageal injury by regenerating esophageal tissues [ 87 ].…”

Section: Application Of Dpscs and Tissue Engineering Applicationsmentioning

confidence: 99%

Recycle the dental fairy’s package: overview of dental pulp stem cells

Yang

Xiao

et al. 2018

Stem Cell Res Ther

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Adult stem cells are excellent cell resource for cell therapy and regenerative medicine. Dental pulp stem cells (DPSCs) have been discovered and well known in various application. Here, we reviewed the history of dental pulp stem cell study and the detail experimental method including isolation, culture, cryopreservation, and the differentiation strategy to different cell lineage. Moreover, we discussed the future potential application of the combination of tissue engineering and of DPSC differentiation. This review will help the new learner to quickly get into the DPSC filed.

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Section: Application Of Dpscs and Tissue Engineering Applicationsmentioning

confidence: 99%

Recycle the dental fairy’s package: overview of dental pulp stem cells

Yang

Xiao

et al. 2018

Stem Cell Res Ther

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“…Dental pulp stem cells (DPSCs), a type of MSC, are characterized by self-renewal, multipotent differentiation potential and amplification in vitro. Under appropriate extracellular stimuli, DPSCs differentiate into various lineages, including osteoblasts, neurons, vascular cells and hepatocytes [ 14 , 15 ]. Numerous studies have revealed that DPSCs could migrate to the lesion site, which accelerates tissue repair and regeneration [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis

Zhang

Nepal

et al. 2021

Stem Cell Res Ther

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Background Ulcerative colitis (UC) is a chronic and recurrent disease without satisfactory treatment strategies. Dental pulp stem cell (DPSC) transplantation has been proposed as a potential therapy for UC. This study aimed to investigate the therapeutic effects of the rat hepatocyte growth factor (HGF) gene transduced into DPSCs for UC. Methods The therapeutic effects of HGF-DPSCs transplanted intravenously into a rat model of UC induced by 5% dextran sulphate sodium (DSS) were compared with the other treatment groups (LV-HGF group, DPSCs group and GFP-DPSCs group). Immunofluorescence and immunohistochemistry were used to observe the localization and proliferation of HGF-DPSCs at the site of colon injury. The expression levels of inflammatory factors were detected by real-time quantitative PCR (RT-PCR) and western blotting. The oxidative stress markers were detected by ELISA. DAI scores and body weight changes were used to macroscopically evaluate the treatment of rats in each group. Results Immunofluorescence and immunohistochemistry assays showed that HGF-DPSCs homed to colon injury sites and colocalized with intestinal stem cell (ISC) markers (Bmi1, Musashi1 and Sox9) and significantly promoted protein expression (Bmi1, Musashi1, Sox9 and PCNA). Anti-inflammatory cytokine (TGF-β and IL-10) expression was the highest in the HGF-DPSCs group compared with the other treatment groups, while the expression of pro-inflammatory cytokines (TNF-α and INF-γ) was the lowest. Additionally, the oxidative stress response results showed that malondialdehyde (MDA) and myeloperoxidase (MPO) expression decreased while superoxide dismutase (SOD) expression increased, especially in the HGF-DPSCs group. The DAI scores showed a downward trend with time in the five treatment groups, whereas body weight increased, and the changes were most prominent in the HGF-DPSCs group. Conclusions The study indicated that HGF-DPSCs can alleviate injuries to the intestinal mucosa by transdifferentiating into ISC-like cells, promoting ISC-like cell proliferation, suppressing inflammatory responses and reducing oxidative stress damage, which provides new ideas for the clinical treatment of UC.

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“…The isolation of MSCs from dental tissues is convenient during the procedures. Hence, they are considered to be potent candidates for bone tissue engineering 5,6 .…”

Section: Introductionmentioning

confidence: 99%

LncRNA H19 promotes the committed differentiation of stem cells from apical papilla via miR-141/SPAG9 pathway

Yan

et al. 2019

Cell Death Dis

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Long noncoding RNAs (lncRNAs) exert significant roles at transcriptional and post-transcriptional levels. Stem cells from apical papilla (SCAPs) differentiate into dentin/bone-like tissues under certain conditions. So far, whether lncRNA-H19 can affect the proliferative behaviors and osteo/odontogenesis of SCAPs, as well as its specific mechanism remain to be elucidated. Here, SCAPs were isolated and transfected with the lentiviruses or packaging vectors. Our results showed that lncRNA-H19 had no significant effect on the proliferative behaviors of SCAPs, as presented by CCK-8 assay, EdU assay and flow cytometry (FCM). Furthermore, alkaline phosphatase (ALP) activity, alizarin red staining, Western blot assay (WB), quantitative real-time polymerase chain reaction (qRT-PCR) and in vivo bone formation assay were conducted to verify the biological influences of H19 on SCAPs. Overexpression of H19 led to the enhanced osteo/odontogenesis of SCAPs, whereas knockdown of H19 inhibited these effects. Mechanistically, H19 competitively bound to miR-141 and prevented SPAG9 from miRNA-mediated degradation, thus significantly elevating phosphorylated levels of p38 and JNK and facilitating the committed differentiation of SCAPs. Taken together, the osteo/odontogenesis of SCAPs was upregulated by overexpression of H19 via miR-141/SPAG9 pathway.

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Therapeutic effect of dental pulp stem cell transplantation on a rat model of radioactivity-induced esophageal injury

Cited by 22 publications

References 53 publications

Recycle the dental fairy’s package: overview of dental pulp stem cells

Recycle the dental fairy’s package: overview of dental pulp stem cells

Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis

LncRNA H19 promotes the committed differentiation of stem cells from apical papilla via miR-141/SPAG9 pathway

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