Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosis

Abstract

Background Liver fibrosis is a major medical problem with high mortality and morbidity rates where the formation of regenerative nodules and cirrhosis leads to loss of liver function and may result in the development of hepatocellular carcinoma. bone marrow mesenchymal stem cells (BM-MSCs) have drawn attention as a novel approach for treatment of liver fibrosis. This study aimed to evaluate the therapeutic effect of BM-MSCs on the liver structure in carbon tetrachloride (CCl 4 ) induced liver fibrosis in male rats relative to resveratrol and Silybum marianum as standard drugs derived from herbal plants. Methods Fifty adult male albino rats (Sprague Dawley strain; 180–220 g mean body weight) were purchased from the Laboratory Animal Unit in the Nile Center of Experimental Research, Mansoura, Egypt. Liver function were determined, isolation and preparation of BM- MSCs and detection of cell-surface markers by flow cytometry. Results Animals exposed to CCl 4 developed liver injury characterized by significant increase of liver enzymes, malondialdehyde (MDA), tumor necrosis factor alpha (TNFα), and CYP450, inhibition of antioxidant enzymes, and decreased albumin. Treatment with stem cells enhanced liver state more effectively than resveratrol and S. marianum . It significantly decreased AST, ALT, ALP, MDA, TNF-α, and CYP450 and increased albumin, SOD, GSH, GST, and CAT. Histopathological study and atomic force microscope results confirmed the therapeutic effects of MSCs. Conclusions BM-MSCs could restore liver structure and function in CCL 4 induced liver fibrosis rat model, ameliorating the toxicity of CCl 4 and improving liver function tests.

Integrated Use of Nanomechanical, Histological, and Biochemical Biomarkers of Oreochromis niloticus as a Signature of Metal Stress

“…Apoptotic cells tend to exhibit higher levels of membrane roughness. Similar findings were observed in rats treated by CCL4 (Khalil et al, 2020) and mouse cells treated by H 2 O 2 (Wang et al, 2009). The present study could be the first study to measure stiffness and roughness in order to evaluate the impact of water pollution at the cellular level.…”

Mechanisms of interactions between lung‐origin telocytes and mesenchymal stem cells to treat experimental acute lung injury

“…Culture medium was changed every 48 h during TCs were expanded and the passages 6–8 were used for cell transplantation. MSCs were obtained, thawed, and expanded from bone marrow of 6–8 weeks C57BL/6J mice 15 . In briefly, the bone marrow was washed repeatedly for the single‐cell suspension and harvested fluid was centrifuged at 500 × g for additional 5 min to acquire the cell mass.…”

“…MSCs were obtained, thawed, and expanded from bone marrow of 6-8 weeks C57BL/6J mice. 15 In briefly, the bone marrow was washed repeatedly for the single-cell suspension and harvested fluid was centrifuged at 500 × g for additional 5 min to acquire the cell mass. After washing thrice, the cells were resuspended and cultured with DMEM/F12 medium, containing 10% fetal bovine serum (FBS) and 1% penicillinstreptomycin in cell incubator.…”

The Role of IL-35 in the Pathophysiological Processes of Liver Disease

“…Liver fibrosis with a disrupted liver architecture was always attributed to the progressive accumulation and reduction of ECM remodeling ( Khalil et al, 2020 ). The formation of liver fibrogenesis was regarded as a wound-healing response, which was driven primarily by various immune response to hurt parenchymal cells.…”