Therapeutic Drug Monitoring of Anticonvulsants in Children in Clinical Practice

Леонова, М. В.; Ивжиц, М. А.; Tischenkova, I. F.; Штейнберг, Л. Л.

doi:10.17749/2077-8333.2017.9.1.026-034

Èpilepsiâ paroksizmalʹnye sostoâ.

2017

DOI: 10.17749/2077-8333.2017.9.1.026-034

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Therapeutic Drug Monitoring of Anticonvulsants in Children in Clinical Practice

М. В. Леонова

М. А. Ивжиц

I. F. Tischenkova

et al.

Abstract: Данная интернет-версия статьи была скачана с сайта http://www.epilepsia.su. Не предназначено для использования в коммерческих целях.Информацию о репринтах можно получить в редакции. Тел.:

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Chromatography-tandem MASS spectrometry (HPLC-MS/MS) for the detection of valproic acid and its metabolites in blood plasma

Malygin¹,

Popov²,

Демидова³

et al. 2018

Èpilepsiâ paroksizmalʹnye sostoâ.

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Aim: to adapt the HPLC-MS/MS technique to determining valproic acid and its metabolites in blood plasma for drug therapy monitoring.Materials and Methods: The chromatographic assay was run using an Agilent 1260 Infinity II chromatograph with a Phenomenex synergi Fusion analytical column 4 μm-C18 2×50 mm. The mobile phase consisted of 0.1% ammonium acetate in distilled water and 0.1% ammonium acetate in methanol (10:90 v/v, 0.5 ml/min). The multiple ions monitoring (MIM) mode was used for mass- spectrometric detection of valproic acid at m/z = 143.1, with the negative ion mode. The method was found applicable over the range from 1 mcg/ml to 200 mcg/ml of valproic acid. For the mass spectroscopy detection of valproic acid metabolites, the multiple reaction monitoring (MRM mode) was used. MS identifications of 2-propyl-4-pentanoil-β-О-glucuronide; 2-propyl-4-pentenoic acid, 3-hydroxy-2- propylpentanoic acid, 4-hydroxy-2-propylpentanoic acid, 2-propylglutaric acid and 3- oxo-2-propylpentanoic acid in the negative ion mode were carried out at m/z 319.2→143.2; m/z 140.1→140.1; m/z 159.1→101; m/z 159.1→123.1; m/z 173→129.1 and m/z 157.05→11, respectively. The method was sensitive over the range from 10 ng/ml to 500 ng/ml of the tested compounds.Results: The developed technique allows for determining valproic acid and its metabolites in a single sample; thus, the preliminary stage of separate sample preparation can be omitted, which increases the informative value of the assay without increasing its cost.Conclusion: This innovative methodology for the quantification of valproic acid and its metabolites in the blood plasma is expected to facilitate the individual approach to the treatment of patients with epilepsy, thereby increasing the efficacy and safety of the pharmacotherapy.

show abstract

Chromatography-tandem MASS spectrometry (HPLC-MS/MS) for the detection of valproic acid and its metabolites in blood plasma

Malygin¹,

Popov²,

Демидова³

et al. 2018

Èpilepsiâ paroksizmalʹnye sostoâ.

View full text Add to dashboard Cite

show abstract

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Therapeutic Drug Monitoring of Anticonvulsants in Children in Clinical Practice

Cited by 1 publication

References 0 publications

Chromatography-tandem MASS spectrometry (HPLC-MS/MS) for the detection of valproic acid and its metabolites in blood plasma

Chromatography-tandem MASS spectrometry (HPLC-MS/MS) for the detection of valproic acid and its metabolites in blood plasma

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