Therapeutic down-regulation of central and peripheral B-cell-activating factor (BAFF) production in pediatric opsoclonus–myoclonus syndrome

Pranzatelli, Michael R.; Tate, Elizabeth D.; Hoefgen, Erik R.; Swan, Jennifer A.; Colliver, Jerry A.

doi:10.1016/j.cyto.2008.06.001

Cited by 38 publications

(34 citation statements)

References 25 publications

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“…In addition, patients with any acute or chronic infection were not included. BAFF levels of our healthy control group are comparable to a group of n050 pediatric control patients described by Pranzatelli et al [23]. The male/female distribution of our healthy controls differed from those of the study group and ESRD patients.…”

Section: Discussionsupporting

confidence: 81%

Elevated serum levels of B-cell activating factor in pediatric renal transplant patients

et al. 2012

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Section: Discussionsupporting

confidence: 81%

Elevated serum levels of B-cell activating factor in pediatric renal transplant patients

et al. 2012

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“…The expression of COX-2 and iNOS is regulated by transcription factors AP-1, NF-κB, and STAT3 [15,30,31]. Nuclear extracts were prepared and EMSA with AP-1 and NF-κB performed.…”

Section: Resultsmentioning

confidence: 99%

“…LPS also induces expression of IL-12 in macrophages through the NF-κB pathway [10,11], and the AP-1 binding site was identified in the promoter region of IL-12p40 [12]. Moreover, LPS-induced expression of COX-2 and inducible nitric oxide synthase (iNOS) is also controlled through NF-κB and AP-1 [13], and LPS-induced expression of iNOS and IL-6 require phosphorylation of STAT3 [14,15]. Therefore, the suppression of the release of pro-inflammatory mediators, through the modulation of intracellular signaling in immune cells, could be used for the treatment or prevention of endotoxemia and sepsis.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-κB and AP-1 signaling

Jedinak

Dudhgaonkar

et al. 2011

Nutr J

106

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BackgroundMushrooms are well recognized for their culinary properties as well as for their potency to enhance immune response. In the present study, we evaluated anti-inflammatory properties of an edible oyster mushroom (Pleurotus ostreatus) in vitro and in vivo.MethodsRAW264.7 murine macrophage cell line and murine splenocytes were incubated with the oyster mushroom concentrate (OMC, 0-100 μg/ml) in the absence or presence of lipopolysacharide (LPS) or concanavalin A (ConA), respectively. Cell proliferation was determined by MTT assay. Expression of cytokines and proteins was measured by ELISA assay and Western blot analysis, respectively. DNA-binding activity was assayed by the gel-shift analysis. Inflammation in mice was induced by intraperitoneal injection of LPS.ResultsOMC suppressed LPS-induced secretion of tumor necrosis factor-α (TNF-α, interleukin-6 (IL-6), and IL-12p40 from RAW264.7 macrophages. OMC inhibited LPS-induced production of prostaglandin E2 (PGE2) and nitric oxide (NO) through the down-regulation of expression of COX-2 and iNOS, respectively. OMC also inhibited LPS-dependent DNA-binding activity of AP-1 and NF-κB in RAW264.7 cells. Oral administration of OMC markedly suppressed secretion of TNF-α and IL-6 in mice challenged with LPS in vivo. Anti-inflammatory activity of OMC was confirmed by the inhibition of proliferation and secretion of interferon-γ (IFN-γ), IL-2, and IL-6 from concanavalin A (ConA)-stimulated mouse splenocytes.ConclusionsOur study suggests that oyster mushroom possesses anti-inflammatory activities and could be considered a dietary agent against inflammation. The health benefits of the oyster mushroom warrant further clinical studies.

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“…Would pre-treatment of RAG1 −/− recipient animals with TACI-Ig, thereby lowering systemic BAFF availability, favor B-cell reconstitution of non-autoreactive B cells at the expense of autoreactive clones? These observations predict that B-cell autoimmunity could be modulated by controlling BAFF availability in the organism and, in fact, BAFF is a current target for therapeutic intervention in autoimmune disorders (Pelletier et al, 2003; Pranzatelli et al, 2008). …”

Section: Discussionmentioning

confidence: 99%

Stromal cell independent B cell development in vitro: Generation and recovery of autoreactive clones

Holl

Haynes

Kelsoe

2010

Journal of Immunological Methods

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We describe and characterize a stromal-cell independent culture system that efficiently supports pro-B cell to IgM+ B-cell development with near normal levels of IgH and Igκ diversity. Pro-B cells present in non-adherent bone marrow cells proliferate in the presence of IL-7 and subsequent to the removal of IL-7 and addition of BAFF, differentiate normally into IgM+ B cells. B-cell development in vitro closely follows the patterns of development in vivo with culture derived (CD) B cells demonstrating characteristic patterns of surface antigen expression and gene activation. IgM+ CD B cells respond to TLR stimulation by proliferation and differentiation into antibody-secreting cells. Self-reactive IgM+ B-cell development is blocked in 3H9 IgH knockin mice; however, cultures of 3H9 IgH knockin pro-B cells yields high frequencies of “forbidden”, autoreactive IgM+ B cells. Furthermore, serum IgG autoantibody exceeded that present in autoimmune, C4−/− animals following the reconstitution of RAG1−/− mice with IgM+ CD cells derived from BL/6 mice.

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Therapeutic down-regulation of central and peripheral B-cell-activating factor (BAFF) production in pediatric opsoclonus–myoclonus syndrome

Cited by 38 publications

References 25 publications

Elevated serum levels of B-cell activating factor in pediatric renal transplant patients

Elevated serum levels of B-cell activating factor in pediatric renal transplant patients

Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-κB and AP-1 signaling

Stromal cell independent B cell development in vitro: Generation and recovery of autoreactive clones

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