Therapeutic Applications of Interleukin 24 (IL24): A Review

Amirzada, Muhammad Imran; Jin, Jian

doi:10.4314/tjpr.v11i6.20

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…Another mechanism by which IL-24 specifically promotes apoptosis is via oxidative stress. This occurs when IL-24, as transfected into prostate cancer cells, increases the reactive oxygen species (ROS) production followed by mitochondrial dysfunction 14,19,20 . Thus, IL-24 exerts tumor-suppressive effects via multiple signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Expression, Purification and Functional Assessment of Smallest Isoform of Human Interleukin-24 in Escherichia coli

Valiyari

Mahdian

Salami

et al. 2017

Braz. arch. biol. technol.

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Interleukin-24 (IL-24) is a novel tumor-suppressor gene that has different alternative splice isoforms. It has been shown that new smallest isoform of human IL-24 gene, lacking three exons, induces higher levels of cytotoxicity than all the isoforms, indicating shortest isoform of IL-24 may be a new promising anti-cancer agent. In this study, we aimed to provide a reproducible method for recombinant production of the smallest isoform of IL-24 (sIL-24). The Structure of sIL-24 was analyzed using bioinformatics tools (I-TASSER, Prosa, RAMPAGE and SPDBV version 4.1). The DNA sequence encoding sIL-24 was chemically synthesized and sub-cloned into the pET-32a (+) vector for further protein expression in Escherichia coli BL21 (DE3) strain. Upon IPTG induction, sIL-24 peptide was expressed as a thioredoxin fusion protein. The recombinant sIL-24 was released from the fusion by TEV protease cleavage followed by nickel affinity chromatography. The yield of the purified sIL-24 was estimated about 380 μg/ml. MTT assay showed that sIL-24 peptide inhibited the proliferation of PC-3 cancer cells more effectively than full length IL-24 protein, while none affect the survival of MRC-5 normal cells. These results indicate that the presented expression system is an efficient system for the production of small functional recombinant sIL-24 peptide.This functional peptide may have cancer therapeutic application.

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Section: Introductionmentioning

confidence: 99%