Therapeutic applications of embryonic stem cells

Shufaro, Yoel; Reubinoff, Benjamin

doi:10.1016/j.bpobgyn.2004.07.002

Cited by 42 publications

(24 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The therapeutic uses of human embryonic stem cells that are derived from viable pre-embryos are very promising (8)(9)(10)(11). However, some countries argue against this usage because of the ethical dilemmas it may cause.…”

mentioning

confidence: 98%

Human parthenogenetic blastocysts derived from noninseminated cryopreserved human oocytes

Fried

Ross

Zang

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

mentioning

confidence: 98%

Human parthenogenetic blastocysts derived from noninseminated cryopreserved human oocytes

Fried

Ross

Zang

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

“…In some cases the transplanted cells also gave rise to glia and astrocytes. This procedure has been shown to promote recovery in animal models of Parkinson's Disease and spinal cord injury [108].…”

Section: A Human Pluripotent Stem Cellsmentioning

confidence: 99%

Differentiation of neural lineage cells from human pluripotent stem cells

Schwartz

Brick

Stover

et al. 2008

Methods

View full text Add to dashboard Cite

Human pluripotent stem cells have the unique properties of being able to proliferate indefinitely in their undifferentiated state and to differentiate into any somatic cell type. These cells are thus posited to be extremely useful for furthering our understanding of both normal and abnormal human development, providing a human cell preparation that can be used to screen for new reagents or therapeutic agents, and generating large numbers of differentiated cells that can be used for transplantation purposes. Critical among the applications for the latter are diseases and injuries of the nervous system, medical approaches to which have been, to date, primarily palliative in nature. Differentiation of human pluripotent stem cells into cells of the neural lineage, therefore, has become a central focus of a number of laboratories. This has resulted in the description in the literature of several dozen methods for neural cell differentiation from human pluripotent stem cells. Among these are methods for the generation of such divergent neural cells as dopaminergic neurons, retinal neurons, ventral motoneurons, and oligodendroglial progenitors. In this review, we attempt to fully describe most of these methods, breaking them down into five basic subdivisions: 1) starting material, 2) induction of loss of pluripotency, 3) neural induction, 4) neural maintenance and expansion, and 5) neuronal/glial differentiation. We also show data supporting the concept that undifferentiated human pluripotent stem cells appear to have an innate neural differentiation potential. In addition, we evaluate data comparing and contrasting neural stem cells differentiated from human pluripotent stem cells with those derived directly from the human brain.

show abstract

“…9 Based on the limitations of other solutions in repairing major critical bone defects, 3,4,10-15 stem cell-based therapies became of major interest, in spite of several practical and safety issues. [16][17][18][19][20][21][22][23][24] Caplan and colleagues initiated the use of mesenchymal stem cells (MSCs) with scaffolds to produce bone matrix after implantation, 25 but the adequate cell delivery remains a problem. 21,26 Single cells injected in trypsinized form are not expected to survive at a high rate after direct injection.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Implantation of Bone Marrow–Derived Mesenchymal Stem Cells with Fibrin Micro Beads to Repair a Critical-Size Bone Defect in Mice

Ben-Ari

Rivkin

Frishman

et al. 2009

Tissue Engineering Part A

View full text Add to dashboard Cite

Fibrin microbeads (FMBs) made using thermal treatment of fibrin drops in oil can efficiently isolate mesenchymal stem cells (MSCs) from bone marrow (BM) and other similar sources and culture them continuously in suspension culture. The pure mesenchymal profile of MSCs isolated using FMBs and their differentiation potency to different mesenchymal lineages were previously described in detail. In the current study, MSCs were isolated from the BM of (GFP+) C57/bl mice using FMBs. Addition of pro-osteogenic medium with 10 mM of ss-glycerolphosphate, 50 microg/mL of ascorbic acid, and 10(-8) M of dexamethasone for 1 month resulted in ossified bone-like solid cellular structures, as seen using fluorescence and scanning electron microscopy (SEM). Such spontaneously formed structures were implanted in full-depth approximately 5-mm-diameter drilled defects in the skulls of wild-type c57/bl mice. Two months later, the excised upper parts of the skulls with the defects were viewed using fluorescence microscopy for green fluorescence protein of the cells in the defect and using SEM. They were also scanned using micro-computed tomography to visualize the formation of new hard tissue. Then the samples were processed and sectioned for hematoxylin and eosin staining and immunohistochemistry. Implanted FMBs loaded with (GFP+) MSCs formed partially mature, dense bone-like tissue using a residual moderate inflammatory process containing remnants of FMBs and neo-angiogenesis. The filled defect with bone-like tissue had a Ca/P ratio similar to that of native bone. Limited merging of the implant with the skull indicated that the induced bone regeneration derived from the MSCs that were delivered with the implant. No repair was seen in the control animals without implants or where the defect was filled with FMBs only. Repair scoring (on a 0-5 scale) was found to be 3.38+/-0.35 in the experimental arm, relative to 0 in the controls (p < 0.001).

show abstract

Therapeutic applications of embryonic stem cells

Cited by 42 publications

References 77 publications

Human parthenogenetic blastocysts derived from noninseminated cryopreserved human oocytes

Human parthenogenetic blastocysts derived from noninseminated cryopreserved human oocytes

Differentiation of neural lineage cells from human pluripotent stem cells

Isolation and Implantation of Bone Marrow–Derived Mesenchymal Stem Cells with Fibrin Micro Beads to Repair a Critical-Size Bone Defect in Mice

Contact Info

Product

Resources

About